LOCUS       sequence-315414-        2281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: lvl0 APT1t
FEATURES             Location/Qualifiers
     source          1..2281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      16..73
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(97..114)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(268..856)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(348..367)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1039..1133)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1154..1813)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1728..1747
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1975..1993
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1997..2040)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 cttgagaccc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
       61 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
      121 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      181 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      241 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
      301 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      361 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      421 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      481 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      541 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      601 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      661 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      721 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      781 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      841 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      901 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
      961 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1021 tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     1081 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     1141 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     1201 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     1261 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     1321 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     1381 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     1441 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     1501 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     1561 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     1621 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1681 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1741 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     1801 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     1861 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact
     1921 cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     1981 cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
     2041 tggaattcgg tctctgcttc aagaaactgg aagagaaggt tattggatcg agtgttgatg
     2101 ctatttttca tgtatggtga gacattttgc gtgggatttg atccttgttg tttcaactta
     2161 tcataattgg ttcagactag aaaatggcat ttgaatgtca ggattcgatt gcagtttcct
     2221 attgttccac ttaaaatccc cgattaataa ggtgttatgg gttttattta tagaatcaac
     2281 g
//
