LOCUS       sequence-31542-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Cp-LSm5
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(440..457)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(467..484)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             503..525
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    540..564
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(564..583)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(565..583)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(667..686)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tccttcgggc ttgtagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttcg tcatcaagaa aagttaaagc tgtctggatc ccctccagga accaacattg
      121 ctacattatt cccactcaac aatatagtct ctaaacgatt caccatcact ctttttaact
      181 tcttgtttcc tcctgatatg tcctcctcat ctgccttaaa cccatactcc tgaacatcat
      241 ccaataccat atttacatat tcgtcaaatc ctcttagcac tccactaaac tctttatctc
      301 ctttcatcac cacatatatt ctattcccaa tacacttgtc tatcagtgct aatggaagga
      361 ttatattccc tcccttctga ttacttcctc cctgagattt attagctggt gtctcgctca
      421 tgtaatttac tttataacta ctaccacgcg gaaccaggcc gctgctgtga tgatgatgat
      481 gatggctgct gcccatggta tatctccttc ttaaagttaa acaaaattat ttctagaggg
      541 gaattgttat ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga
      601 tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
      661 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
      721 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
      781 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
      841 tggggctgct tcctaatgca ggagtcgcat aagggagagc gtcgagat
//
