LOCUS       sequence-31543-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Cp-NHP2
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(461..478)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(488..505)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             524..546
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    561..585
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(585..604)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(586..604)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(688..707)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttcgtcatc
       61 atactagaat ctgttctatt tgatctttta gctcagtaat ctgagaagac aagctggatc
      121 catccttaga tgtaatcgct gctgctataa ctggtctaga tactccacaa gcacgaccca
      181 aagctacctt tgatcggaca aaaacataag gagtattctt atcttcacaa actaatggga
      241 gatgaagaag aatctccaaa ggctcagcat ctgcagcaag taaaactatc tcagctatac
      301 ccctatttaa agcctttgtt gcttcatttg ctccctttct tagttgctta tagttacaag
      361 cttgctgtac caagttaata atcttgttgt tcaagtctgg ggaggctaat ggaaatgcct
      421 tagggttaaa acctgtatct tcacttgcct cgttttggct actaccacgc ggaaccaggc
      481 cgctgctgtg atgatgatga tgatggctgc tgcccatggt atatctcctt cttaaagtta
      541 aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt
      601 attaatttcg cgggatcgag atctcgatcc tctacgccgg acgcatcgtg gccggcatca
      661 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
      721 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
      781 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
      841 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
//
