LOCUS       sequence-31544-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Py-U5-15kD
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..41)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(494..511)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(521..538)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             557..579
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    594..618
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(618..637)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(619..637)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(721..740)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gggcttgtag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcaagc
       61 ttcgtcatca atatttatat ttagttgaat aatcctttgg tgatatgact aaacctcttc
      121 cttttcttgc acctctgaat atggtttcaa caatatcaat aaattcttgt ttattattca
      181 taggccaatt aattttatta ttattacctg tacctaaatc tatcaacatg tgtttatttc
      241 tataaaaaaa cataacagaa actggatcat acaattcata cattgtatta aaatcaggaa
      301 cttcggtaat atccaccaaa tatataacac aaaaattttt tatatcatca gctactttat
      361 atagtaattc atccattttc atacaatcag gatcgtaatc atgaccaaaa cgaatacaaa
      421 ctaatcgttc atcttcgttt actatagctt gatcaactgc ccaaccacta tttaaatgct
      481 gaagcataaa cgaactacca cgcggaacca ggccgctgct gtgatgatga tgatgatggc
      541 tgctgcccat ggtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg
      601 ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga
      661 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
      721 cctatatcgc cgacatcacc gatggggaag atcggggctc gccacttcgg gctcatgagc
      781 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
      841 tccttgcatg ca
//
