LOCUS       sequence-315443-        7037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Untagged WDR24 cDNA expression vector for transfections.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Orozco JM, Krawczyk PA, Scaria SM, Cangelosi AL, Chan SH, Kunchok T,
            Lewis CA, Sabatini DM
  TITLE     Dihydroxyacetone phosphate signals glucose availability to mTORC1.
  JOURNAL   Nat Metab. 2020 Sep;2(9):893-901. doi: 10.1038/s42255-020-0250-5. 
            Epub 2020 Jul 27.
  PUBMED    32719541
REFERENCE   2  (bases 1 to 7037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-020-0250-5"; journalName: "Nat Metab"; date: 
            "2020-09"; volume: "2"; issue: "9"; pages: "893-901"
COMMENT     Sequence Label: WDR24 in pRK5
FEATURES             Location/Qualifiers
     source          1..7037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      912..921
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3324..3333
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3337..3471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3374..3393)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3428..3447
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        3540..3897
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3540..3731
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3563..3583)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3748..3883
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3917..3934)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3917..3933)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3926..3948)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4146..4601
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4233..4252)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4443..4464
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4618..4637)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4737..4759
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4797..4815)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4883..4987
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4988..5056,5057..5848)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5206..5225)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6019..6607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6508..6527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6761..6778
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6895..6916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6931..6936,6937..6954,6955..6961)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6969..6985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6974..6996
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6993..7009
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6993..7009
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgattgaatt cgccaccatg gagaagatgt cccgtgtgac cacagccctg ggtggcagcg
      961 tgctgacagg ccgcaccatg cactgccacc tggatgctcc cgccaatgcc atcagtgtgt
     1021 gccgcgacgc agcccaggtg gtcgtggcag gccgtagcat cttcaagatc tatgccatcg
     1081 aggaggaaca gttcgtggaa aagctgaacc tgcgtgtggg gcgcaagcct tcgcttaacc
     1141 tgagctgtgc tgacgtggtc tggcaccaga tggatgagaa cctgctggcc acagcagcca
     1201 ccaatggcgt ggtggtcacg tggaacctgg gccggccatc ccgcaacaag caggaccagc
     1261 tgttcacaga acacaagcgc acggtaaaca aagtctgctt ccaccccacc gaagcccacg
     1321 tgctgctcag tggctcccag gatggcttca tgaagtgctt tgacctccgc agaaaggact
     1381 ctgtcagcac cttctcgggc cagtcggaga gcgtgcggga cgtgcagttc agtatccggg
     1441 actacttcac cttcgcctcc acctttgaga acggcaatgt gcagctctgg gacatccggc
     1501 gtcccgaccg gtgcgagagg atgttcacag cccacaacgg acccgtcttc tgctgcgact
     1561 ggcaccccga ggacaggggc tggttggcca ctggagggcg cgacaagatg gtgaaggtct
     1621 gggacatgac cacgcaccgt gccaaggaga tgcactgtgt gcagaccatc gcctcggtgg
     1681 cccgtgtgaa gtggcggcca gagtgccgcc accacctggc cacgtgctcc atgatggtgg
     1741 accacaacat ctatgtttgg gacgtgcgcc ggcccttcgt gccagctgcc atgtttgagg
     1801 aacaccgaga cgtcaccacg ggaattgcct ggcgccaccc ccacgacccc tccttcctgc
     1861 tgtctggctc caaggacagc tcgctgtgcc agcacctgtt ccgcgacgcc agccagcccg
     1921 tcgagcgcgc caaccctgag ggcctctgct acggcctctt cggggacctg gccttcgccg
     1981 ccaaggagag cctcgtggct gccgagtcgg ggcgcaagcc ctacactggc gaccggcgcc
     2041 accccatctt ctttaagcgc aagctggacc ctgccgagcc cttcgcaggc ctcgcctcca
     2101 gtgccctcag tgtctttgag acggagccag gtggcggcgg catgcgctgg tttgtggaca
     2161 cagctgagcg ttatgcgctg gctggccggc cactggccga gctctgtgac cacaacgcaa
     2221 aggtggctcg agagcttggc cgcaaccagg tggcgcaaac gtggaccatg ctgcggatca
     2281 tctactgcag ccctggccta gtgcccactg caaacctcaa ccacagtgtg ggcaagggtg
     2341 gctcctgtgg cctcccgctc atgaacagtt tcaacctgaa ggatatggcc ccagggttgg
     2401 gcagtgagac gcggctggac cgcagcaaag gagatgcgcg gagcgacaca gttctgctcg
     2461 actcctcggc cacactcatc accaatgagg ataacgagga aaccgagggc agcgacgtac
     2521 ctgccgacta cctgctgggt gacgtggaag gtgaggagga cgagctgtac ctgctggatc
     2581 cggaacacgc gcaccccgag gaccctgagt gcgtgctgcc gcaggaggcc tttccgctgc
     2641 gccacgagat cgtggacacg cctcccgggc ccgagcacct gcaggacaag gccgactccc
     2701 cgcacgtgag cggcagcgag gcggatgtgg cctccctggc ccccgtggac tcctccttct
     2761 cgctcctgtc tgtctcacac gcgctctacg acagccgcct gccgcccgac ttcttcggcg
     2821 tgctggtgcg cgacatgctg cacttctacg ctgagcaggg cgacgtgcag atggctgtgt
     2881 ctgtgctcat cgtcctgggt gaacgggtgc gcaaggacat cgacgagcag acccaggagc
     2941 actggtacac ttcctacatc gacctgctgc agcgcttccg cctctggaac gtgtccaacg
     3001 aggtggtcaa gctgagcacc agccgcgccg tcagctgcct caaccaggcc tccaccaccc
     3061 tgcacgtcaa ctgcagccac tgcaagcggc ccatgagcag ccggggctgg gtctgcgaca
     3121 ggtgccaccg ctgcgccagc atgtgtgccg tctgccacca cgtagtcaag ggtctcttcg
     3181 tgtggtgcca gggctgcagc cacggcggcc acctgcagca catcatgaag tggctggaag
     3241 gcagctccca ctgtcccgca ggctgcggcc acctctgcga gtactcctga gcggccgcta
     3301 agtaagtaag gatccccagc ttggccgcca tggcccaact tgtttattgc agcttataat
     3361 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3421 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat cgggaattaa
     3481 ttcggcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt aggtaccttc
     3541 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     3601 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     3661 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3721 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3781 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     3841 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     3901 ctgttaacag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     3961 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     4021 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     4081 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     4141 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4201 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4261 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4321 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     4381 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4441 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
     4501 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4561 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     4621 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     4681 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     4741 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     4801 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     4861 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4921 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4981 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5041 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5101 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5161 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5221 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5281 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5341 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5401 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5461 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5521 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5581 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5641 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5701 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5761 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5821 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5881 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5941 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6001 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6061 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6121 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     6181 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6241 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6301 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6361 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6421 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6481 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6541 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6601 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6661 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6721 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     6781 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6841 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     6901 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     6961 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     7021 ttaattcgag ctcgccc
//
