LOCUS       sequence-315445-        8525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-off lentivirus construct all-in-one vector, with Blast 
            resistance, for expression of GAPDH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Orozco JM, Krawczyk PA, Scaria SM, Cangelosi AL, Chan SH, Kunchok T,
            Lewis CA, Sabatini DM
  TITLE     Dihydroxyacetone phosphate signals glucose availability to mTORC1.
  JOURNAL   Nat Metab. 2020 Sep;2(9):893-901. doi: 10.1038/s42255-020-0250-5. 
            Epub 2020 Jul 27.
  PUBMED    32719541
REFERENCE   2  (bases 1 to 8525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-020-0250-5"; journalName: "Nat Metab"; date: 
            "2020-09"; volume: "2"; issue: "9"; pages: "893-901"
COMMENT     Sequence Label: 3XFLAG_GAPDH in pCW57_tTA_Blast
FEATURES             Location/Qualifiers
     source          1..8525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        46..360
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    54..72
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    90..108
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    232..250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    268..286
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     333..357
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      377..386
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             386..451
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        1496..2006
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1925..1943
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2016..2408
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             2418..2471
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2472..3089,3090..3095,3096..3131,3132..3134,3135..3170,
                     3171..3173,3174..3209,3210..3218)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3258..3278)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3676..3687)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3892..4125
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4197..4318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4234..4253)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4288..4307
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4358..4493
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4493..4564
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4565..4633,4634..5425)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4783..4802)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5596..6184
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6085..6104
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6338..6355
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6472..6493
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6508..6513,6514..6531,6532..6538)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6546..6562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6551..6573
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6570..6586
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6570..6586
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6605..6625
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6607..6625
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6653..6879
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6880..7060
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7107..7232
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7725..7958
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8143..8187
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8400..8517
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttacagggac agcagagatc cactttggcc gcgaatcgat atgtcgagtt tactccctat
       61 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
      121 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      181 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      241 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      301 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      361 tggagaattg gctagcgcca ccatggacta caaagaccat gacggtgatt ataaagatca
      421 tgacatcgat tacaaggatg acgatgacaa ggatctcaat ggtggtggtg gtgggtcgac
      481 gatggggaag gtgaaggtcg gagtcaacgg atttggtcgt attgggcgcc tggtcaccag
      541 ggctgctttt aactctggta aagtggatat tgttgccatc aatgacccct tcattgacct
      601 caactacatg gtttacatgt tccaatatga ttccacccat ggcaaattcc atggcaccgt
      661 caaggctgag aacgggaagc ttgtcatcaa tggaaatccc atcaccatct tccaggagcg
      721 agatccctcc aaaatcaagt ggggcgatgc tggcgctgag tacgtcgtag agtccactgg
      781 cgtcttcacc accatggaga aggctggggc tcatttgcag gggggagcca aaagggtcat
      841 catctctgcc ccctctgctg atgcccccat gttcgtcatg ggtgtgaacc atgagaagta
      901 tgacaacagc ctcaagatca tcagcaatgc ctcctgcacc accaactgct tagcacccct
      961 ggccaaggtc atccatgaca actttggtat cgtggaagga ctcatgacca cagtccatgc
     1021 catcactgcc acccagaaga ctgtggatgg cccctccggg aaactgtggc gtgatggccg
     1081 cggggctctc cagaacatca tccctgcctc tactggcgct gccaaggctg tgggcaaggt
     1141 catccctgag ctgaacggga agctcactgg catggccttc cgtgtcccca ctgccaacgt
     1201 gtcagtggtg gacctgacct gccgtctaga aaaacctgcc aaatatgatg acatcaagaa
     1261 ggtggtgaag caggcgtcgg agggccccct caagggcatc ctgggctaca ctgagcacca
     1321 ggtggtctcc tctgacttca acagcgacac ccactcctcc acctttgacg ctggggctgg
     1381 cattgccctc aacgaccact ttgtcaagct catttcctgg tatgacaacg aatttggcta
     1441 cagcaacagg gtggtggacc tcatggccca catggcctcc aaggagtaag gatccggggt
     1501 tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
     1561 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
     1621 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
     1681 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
     1741 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
     1801 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
     1861 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
     1921 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
     1981 accgaatcac cgacctctct ccccagcaat tcaccatggt tcctttgtct caagaagaat
     2041 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
     2101 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
     2161 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
     2221 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
     2281 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
     2341 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
     2401 gggagggccc cggtgccgaa ggtagaggtt ctctcctcac ttgtggtgat gttgaagaaa
     2461 accctggtcc aatgtctaga ctggacaaga gcaaagtcat aaactctgct ctggaattac
     2521 tcaatgaagt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg
     2581 agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctggcaa
     2641 tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag
     2701 actttctgcg gaacaacgcc aagtcattcc gctgtgctct cctctcacat cgcgacgggg
     2761 ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc
     2821 tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg
     2881 tgggccactt tacactgggc tgcgtattgg aggatcagga gcatcaagta gcaaaagagg
     2941 aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt
     3001 tcgaccatca gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc
     3061 tggagaaaca gctaaagtgc gaaagcggcg ggccggccga cgcccttgac gattttgact
     3121 tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg
     3181 ctcttgacga ttttgacctt gacatgctcc ccgggtaact aagtaaggat cgatccaaga
     3241 tatcgtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3301 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3361 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3421 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3481 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3541 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     3601 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3661 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3721 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3781 gccgcctccc cgcctgtttc gcctcggcgt ccggactaga ggtaccttta agaccaatga
     3841 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     3901 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     3961 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4021 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     4081 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     4141 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     4201 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4261 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4321 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4381 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4441 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctttcaaa
     4501 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4561 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4621 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4681 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4741 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4801 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4861 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4921 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4981 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5041 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5101 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5161 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5221 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5281 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5341 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5401 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5461 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5521 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5581 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5641 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5701 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     5761 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5821 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5881 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5941 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6001 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6061 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6121 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6181 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6241 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6301 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6361 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     6421 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     6481 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     6541 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     6601 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     6661 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     6721 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     6781 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     6841 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     6901 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6961 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7021 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     7081 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     7141 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     7201 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     7261 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     7321 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     7381 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     7441 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     7501 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     7561 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     7621 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     7681 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     7741 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     7801 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     7861 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     7921 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     7981 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     8041 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     8101 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     8161 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     8221 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     8281 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     8341 ctcccaaccc cgaggggaca attctcgacc tcgagacaaa tggcagtatt catccacaat
     8401 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     8461 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     8521 gttta
//
