LOCUS       sequence-31545-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Cp-NTF2
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             151..168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             178..195
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             609..626
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(679..697)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      693..740
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag cagcggcctg
      181 gttccgcgtg gtagtgatca aagcattaat ctgaaccctc aattcgacca aattggtaaa
      241 caatttgtac aacattatta ccaaacattt caaacaaata gacctgctct tggaggatta
      301 tatggtcccc aatcaatgtt aacatgggaa gatactcaat tccaaggtca agccaatatt
      361 gttaacaagt ttaattctct taatttccaa agagtccaat ttgaaatcac tcgtgtagac
      421 tgccaacctt ctccaaacaa tggttcaata gtttttgtta ctggagatgt tagaattgat
      481 gatggccaac ctttgaaatt ttcacaagtc ttcaatctta tgccttctgg gaacggtgga
      541 ttcatgattt ttaacgatct atttagacta aatcttggtt gatgacgaag cttgcggccg
      601 cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
      661 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      721 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc
      781 gccctgtagc ggcgcattaa gcgcggcggg ggtggtggtt a
//
