LOCUS       sequence-315452-        7775 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0225:Dendra
FEATURES             Location/Qualifiers
     source          1..7775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3719..3742
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3999..4799
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      4924..5512
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5413..5432
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgagcacccc cgacgcaccg accgaagcca ttccgtcggc
      121 acacggcaag tacaccaacc acggcggcat cgccaagctc atccgcacgc tggcggtgcc
      181 gataatcctg gtctgggtcg ccctcatcgc cgtgctgaac acgatcgtcc cccagctcga
      241 agaggtcggg gagatgcggt cggtgtcgat gagccccgac gacgcaccct cgatgatcgc
      301 gatgaagcgc gtcggcgagg tgttcgaaga gttcaagtcc aacagctcgg tgatgatcgt
      361 gctggagggc gagcagcccc tcggcgacga ggcacacaag tactacgacg agatcgtcga
      421 caagctcgag gccgacaaga cgcacatcga gcacgtccag gacttctggg gcgacccgct
      481 caccgcctct ggcgcgcaga gctccgacgg cctcgcgtcg tacgtgcagg tctacaccgc
      541 gggcaaccag ggtgaggcgc tggccaacga gtcggtcaag gccgtccagg acatcgtcga
      601 cagcgtccag gcgcccccgg gcgtcacggc gtacgtgacc ggaccggccg ccatgtcggc
      661 cgatcagcag atcgccggtg accgctcgat gcggatgatc gaagcgctga ccttcacggt
      721 catcatcatc atgctgctga tggtctaccg ctcgatcatc acggtgatcc tgtcgctggt
      781 gatggtggtg ttctccctcg ccgccgcgcg cggcgtcatc gccttcctcg gctactacga
      841 gatcatcggc ctgtcgacgt tcgccaccaa cctgctggtg acgctggcga tcgccgcggc
      901 gaccgattac gcgatctttt tgataggccg ttatcaagag gccagaagtg tcggcgagag
      961 ccgcgaacag gcgtactaca cgatgttcca cagcacggcg cacgtcgtgc tgggttccgg
     1021 catgaccatc gcgggcgcca cgctgtgcct gcacttcacc cgcatgccgt acttccagtc
     1081 gctgggcatc ccgctggcca tcggcatgac tgtcgtggtg ctcacggccc tggccgtcgg
     1141 acccgccatc atcacagtgg ccagccggtt cggcaagacg ctcgagccca agcgtgcgat
     1201 gcgcacccgc ggctggcgca aagtcggtgc ggccgtggtg cgttggccgg gtccgatcct
     1261 ggtggccgcg gtcgggttgt cgatgatcgg cctgctggcg ctgccgggct accgcaccaa
     1321 ctacaacgac cgtgactacc agcccgccga ccttccggcc aacagcggct acgccgcggc
     1381 cgaccggcac ttctcgcagg cccggatgaa cccggaactg ctgatgatcg agtcggacca
     1441 cgacctgcgc aacagcgccg acttcctggt gatcgagcgc atcgccaagc gcgtcgtcgg
     1501 ggttccgggt gtctcacggg tgcaggccat cacgcgtccg cagggcacgc ccatcgagca
     1561 cacctcgatc ccgttcacca tcagcatgca gggcaccacg cagaccatga accagaagta
     1621 catgcaggat cgcatggcgg acatgctggt tcaggccgat cagatgcagg tcaccgtcga
     1681 caccatgacc aagatgatcg cgctcatgga gcagatgcgc gacaccatga acagcatggt
     1741 gggcaagatg cacggcatgg tcgacgacat caaggaattg cgcgacaaca tcagcgattt
     1801 cgacgatttc ttccggccga tccgcaacta cctgtactgg gaaccgcact gctacaacat
     1861 cccgatgtgc tactccctgc ggtcgatttt cgacaccatg gacggaatcg acaccatgac
     1921 agcggatttc gagcagatcg tccctgacat ggaccgcatg aacgcactga tcccggtgat
     1981 gatcgcgaac atggaaccca tgatcacgac catgaagacc atgaagacca tgatgctgac
     2041 catgcaggcc accaacgccg gtcttcagga tcagatggcc gcgatgcagg agaactccac
     2101 cgcgatgggt gaggccttcg acaaggccaa gaacgacgac tcgttctacc tgccgccgga
     2161 gaccttcgac aacccggact tcaagcgcgg catgaagatg ttcctgtcgc ccgacgggca
     2221 cgcggtccgt ttcatcatca gccacgaggg cgatccgatg agcccggaag gcatccagaa
     2281 gatcgacgac atcaagctcg cggcgaagga ggccatcaag ggcacgccgc tggagggctc
     2341 gaagatctac ctcggcggca ccgcggcgac cttcaaggac atgcaggaag gcgccaacta
     2401 cgacctgatc atcgccggca tcgcggcgct gtgcctcatt ttcatcatca tgctgatcat
     2461 cacgcgggcc gtggtggcct cggcggtgat cgtcggcacg gtggtgatct ccctcggcag
     2521 cgcgttcggc ctgtcggtgc tcatctggca gcacctggtc gggttggaac tgcactggat
     2581 ggtgctggcc atggcggtga tcgtgctgct ggccgtcggt gccgactaca acctgctgct
     2641 ggtgtcacgc atcaaggagg agatccacgc cggcctcgac acgggcatga tccgcgccat
     2701 gggcggcagc ggttcagtgg tgaccgcagc cggtctggtg ttcgcattca ccatgatgtc
     2761 catggccgta agcgaactgg cgatcatcgg ccaggtcggc accacgatcg gcctgggcct
     2821 gctgttcgac accctggtga tccgctcgtt catgacgccg tcgatcgcgg ccctgctggg
     2881 caagtggttc tggtggcccc agatggtgcg ccggcgcccg aagccctcgc cgtggccgaa
     2941 gccgattcag cgcgaaccgg cagacgccgt caacaagctt ggtgcggccg cgggcaacac
     3001 gcctggcatc aatctgatca aggaggacat gcgtgtcaag gtgcatatgg agggcaacgt
     3061 gaacggtcac gccttcgtga tcgaggggga gggtaagggt aagccttacg aagggaccca
     3121 gaccgccaac ctcaccgtca aggagggcgc ccctctccca ttctcgtacg atatcctcac
     3181 taccgcggtg cattacggga accgcgtgtt caccaagtac cccgaggata tccctgacta
     3241 cttcaagcag agcttcccgg agggatactc ctgggagcgg acgatgacct tcgaagacaa
     3301 gggtatctgt acgatccgca gcgacatctc gctggaaggg gactgtttct tccagaacgt
     3361 ccgtttcaag gggaccaact ttccgccgaa cggcccggtc atgcagaaga agaccctcaa
     3421 gtgggagccg agcacggaga agctgcacgt tcgggacggc ctcctggtcg gcaacatcaa
     3481 catggcgctg ctgctggaag gcggcggcca ctacctatgc gacttcaaga ccacgtacaa
     3541 agcgaagaag gtcgtccagc tgcccgacgc gcacttcgtg gaccaccgca tcgaaatcct
     3601 ggggaacgat tcggactaca acaaggtcaa gttgtacgag cacgctgtgg cacgctatag
     3661 cccgctcccg tcgcaggtct ggttcgaagg cggcggcggg gagaacctgt atttccagga
     3721 ctacaaggac gacgacgaca agtgatttaa ataacccctt ggggcctcta aacgggtctt
     3781 gaggggtttt ttgttaacta gcgtacgatc gaattcctgc agctcacggt aactgatgcc
     3841 gtatttgcag taccagcgta cggcccacag aatgatgtca cgctgaaaat gccggccttt
     3901 gaatgggttc atgtgcagct ccatcagcaa aaggggatga taagtttatc accaccgact
     3961 atttgcaaca gtgccgttga tcgtgctatg atcgactgat gtcatcagcg gtggagtgca
     4021 atgtcgtgca atacgaatgg cgaaaagccg agctcatcgg tcagcttctc aaccttgggg
     4081 ttacccccgg cggtgtgctg ctggtccaca gctccttccg tagcgtccgg cccctcgaag
     4141 atgggccact tggactgatc gaggccctgc gtgctacgct gggtccggga gggacgctcg
     4201 tcatgccctc gtggtcaggt ctggacgacg agccgttcga tcctgccacg tcgcccgtta
     4261 caccggacct tggagttgtc tctgacacat tctggcgcct gccaaatgta aagcgcagcg
     4321 cccatccatt tgcctttgcg gcagcggggc cacaggcaga gcagatcatc tctgatccat
     4381 tgcccctgcc accttactcg cctgcaagcc cggtcgcccg tgtccatgaa ctcgatgggc
     4441 aggtacttct cctcggcgtg ggacacgatg ccaacacgac gctgcatctt gccgagttga
     4501 tggcaaaggt tccctatggg gtgccgagac actgcaccat tcttcaggat ggcaagttgg
     4561 tacgcgtcga ttatctcgag aatgaccact gctgtgagcg ctttgccttg gcggacaggt
     4621 ggctcaagga gaagagcctt cagaaggaag gtccagtcgg tcatgccttt gctcggttga
     4681 tccgctcccg cgacattgtg gcgacagccc tgggtcaact gggccgagat ccgttgatct
     4741 tcctgcatcc gccagaggcg ggatgcgaag aatgcgatgc cgctcgccag tcgattggct
     4801 gagctcatga gcggagaacg agatgacgtt ggaggggcaa ggtcgcgctg attgctgggg
     4861 caacacgggg gatccactag ttccatgagc gtcagacccc gtagaaaaga tcaaaggatc
     4921 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4981 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5041 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     5101 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5161 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5221 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5281 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5341 ggggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5401 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5461 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5521 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5581 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     5641 tcgccgcagc cgaacgaccg agcgcaacgc gtgcggccgc ggtacccggg gatcctctag
     5701 agtcgaccac caagggcacc atctctgctt gggccacccc gttggccgca gccagctcgc
     5761 tgagagccgt gaacgacagg gcgaacgcca gcccgccgac ggcgagggtt ccgaccgctg
     5821 caactcccgg tgcaaccttg tcccggtcta ttctcttcac tgcaccagct ccaatctggt
     5881 gtgaatgccc ctcgtctgtt cgcgcaggcg gggggctcta ttcgtttgtc agcatcgaaa
     5941 gtagccagat cagggatgcg ttgcaaccgc gtatgcccag gtcagaagag tcgcacaaga
     6001 gttgcagacc cctggaaaga aaaatggcca gagggcgaaa acaccctctg accagcggag
     6061 cgggcgacgg gaatcgaacc cgcgtagcta gtttggaaga atgggtgtct gccgaccaca
     6121 tatgggccgg tcaagatagg tttttacccc ctctcggctg catcctctaa gtggaaagaa
     6181 attgcaggtc gtagaagcgc gttgaagcct gagagttgca caggagttgc aacccggtag
     6241 ccttgttcac gacgagagga gacctagttg gcacgtcgcg gatggggatc gctgaagact
     6301 cagcgcagcg ggaggatcca agcctcatac gtcaacccgc aggacggtgt gaggtactac
     6361 gcgctgcaga cctacgacaa caagatggac gccgaagcct ggctcgcggg cgagaagcgg
     6421 ctcatcgaga tggagacctg gacccctcca caggaccggg cgaagaaggc agccgccagc
     6481 gccatcacgc tggaggagta cacccggaag tggctcgtgg agcgcgacct cgcagacggc
     6541 accagggatc tgtacagcgg gcacgcggag cgccgcatct acccggtgct aggtgaagtg
     6601 gcggtcacag agatgacgcc agctctggtg cgtgcgtggt gggccgggat gggtaggaag
     6661 cacccgactg cccgccggca tgcctacaac gtcctccggg cggtgatgaa cacagcggtc
     6721 gaggacaagc tgatcgcaga gaacccgtgc cggatcgagc agaaggcagc cgatgagcgc
     6781 gacgtagagg cgctgacgcc tgaggagctg gacatcgtcg ccgctgagat cttcgagcac
     6841 taccggatcg cggcatacat cctggcgtgg acgagcctcc ggttcggaga gctgatcgag
     6901 cttcgccgca aggacatcgt ggacgacggc atgacgatga agctccgggt gcgccgtggc
     6961 gcttcccgcg tggggaacaa gatcgtcgtt ggcaacgcca agaccgtccg gtcgaagcgt
     7021 cctgtgacgg ttccgcctca cgtcgcggag atgatccgag cgcacatgaa ggaccgtacg
     7081 aagatgaaca agggccccga ggcattcctg gtgaccacga cgcagggcaa ccggctgtcg
     7141 aagtccgcgt tcaccaagtc gctgaagcgt ggctacgcca agatcggtcg gccggaactc
     7201 cgcatccacg acctccgcgc tgtcggcgct acgttcgccg ctcaggcagg tgcgacgacc
     7261 aaggagctga tggcccgtct cggtcacacg actcctagga tggcgatgaa gtaccagatg
     7321 gcgtctgagg cccgcgacga ggctatcgct gaggcgatgt ccaagctggc caagacctcc
     7381 tgaaacgcaa aaagcccccc tcccaaggac actgagtcct aaagaggggg gtttcttgtc
     7441 agtacgcgaa gaaccacgcc tggccgcgag cgccagcacc gccgctctgt gcggagacct
     7501 gggcaccagc cccgccgccg ccaggagcat tgccgttccc gccagaaatc tagacggtga
     7561 ccacaacgcg cccgctttga tcggggacgt cgaaatattg gatcgtcggc accgtcacgg
     7621 ccgtgggagg cggcacgatc cgcgacgtga tgatcggccg catccccacg gtgctgcgca
     7681 gtgagctcta cgccatcccg gcgttgatct gtgcgttcgc acgcacaggc ccggtgtgag
     7741 aagggtctct gacgagcggg agaactccct atcag
//
