LOCUS       sequence-315455-        6266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0301:Dendra
FEATURES             Location/Qualifiers
     source          1..6266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2210..2233
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2490..3290
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3415..4003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3904..3923
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggacgcaac cctggcggat gtggaccccg ccctggccga
      121 actggctgct gacctgcctg cccacgcgct gctcaccgat cccgaccaga tcgagggata
      181 tcgccgtgac gccgccgccg atccactcgc gggccgtccg cgcgccgtgg tccgggcgac
      241 ctgcaccgcc gatgtgcaga gcgtcatgcg gtgggcgacg cgtcatcgcg tcgcggtggt
      301 ccccaggggc gccgggtcgg ggctctccgg cggcgcgagc gcagtcgacg gatgcgtcgt
      361 cctgagcacc gagcgcatgc gtgcgatccg gatcgacccg gcgacccgcg tggccgtcgt
      421 gcaacccggc gcgctgaacg ccgaggtcaa gcaggccgca gcagagcaca gcctgtggta
      481 tccgccggat ccggcctcgg tcgacatctc ctcgatcggc ggcaacgccg ccaccaacgc
      541 gggcggcctg tgctgcgtga agtacggcgt caccaccgac tacgtcctcg gcatgcaggt
      601 ggtgctcgcc gacggcacgg ccgtgacact cggcggtccg cgactcaaag acgttgcggg
      661 cctgtccctg accaaactgt tcgtcggttc ggagggcatt ctcggggtga tcaccgaggt
      721 caccctgcgg ctgattccgg cccaggcgtc gccgtccacc gtggtcgcga tgttcgacac
      781 ggtgcaggat gcggccaacg cggtcgtggc catcacggca tcggtgcggc cggccatgct
      841 cgagctgatg gaccacgcgt ccatcaacgc cgtcgaggac tacggccgca tgggtctgga
      901 ccggtcggcg caggcgctgc tgctggtcca gtccgacgcg ccgggtgatg ccgcgtccga
      961 ggtggcacac atcgtcgagg cgtgcgaaaa atcaggtgcc agcgaggttt tcgcgaccga
     1021 tgacccagca gaaggcgccg gtttcacggc cgcgagacgc ctggtcgcgc cggcactcat
     1081 gcaactgggt acggtgctgc tcgaagacgt cacggtgccc atcccgacgc tgcccgcgct
     1141 gctggcaggc atcaaccgga tctccgccga gtgcgacgtg ctgatctgcg tcgtcgcgca
     1201 tgcgggcgac ggaaacaccc accccgtcgt ggttttcgat cccgacgatg ccgaatcgca
     1261 ggccagggcc gaacgcgcct tcagcgaggt catggacctc gcgatcgcga tgggcggcac
     1321 catcaccggt gagcacggcg tcggcaggct caagagagat tggctgccaa agcaactcgg
     1381 cgacgacgtg atgtgggtga cgcgccgcgt caaggacgcc ctggacccgc tcggcatcct
     1441 caacccgggc gctgtcctgc gcccaaagct tggtgcggcc gcgggcaaca cgcctggcat
     1501 caatctgatc aaggaggaca tgcgtgtcaa ggtgcatatg gagggcaacg tgaacggtca
     1561 cgccttcgtg atcgaggggg agggtaaggg taagccttac gaagggaccc agaccgccaa
     1621 cctcaccgtc aaggagggcg cccctctccc attctcgtac gatatcctca ctaccgcggt
     1681 gcattacggg aaccgcgtgt tcaccaagta ccccgaggat atccctgact acttcaagca
     1741 gagcttcccg gagggatact cctgggagcg gacgatgacc ttcgaagaca agggtatctg
     1801 tacgatccgc agcgacatct cgctggaagg ggactgtttc ttccagaacg tccgtttcaa
     1861 ggggaccaac tttccgccga acggcccggt catgcagaag aagaccctca agtgggagcc
     1921 gagcacggag aagctgcacg ttcgggacgg cctcctggtc ggcaacatca acatggcgct
     1981 gctgctggaa ggcggcggcc actacctatg cgacttcaag accacgtaca aagcgaagaa
     2041 ggtcgtccag ctgcccgacg cgcacttcgt ggaccaccgc atcgaaatcc tggggaacga
     2101 ttcggactac aacaaggtca agttgtacga gcacgctgtg gcacgctata gcccgctccc
     2161 gtcgcaggtc tggttcgaag gcggcggcgg ggagaacctg tatttccagg actacaagga
     2221 cgacgacgac aagtgattta aataacccct tggggcctct aaacgggtct tgaggggttt
     2281 tttgttaact agcgtacgat cgaattcctg cagctcacgg taactgatgc cgtatttgca
     2341 gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt
     2401 catgtgcagc tccatcagca aaaggggatg ataagtttat caccaccgac tatttgcaac
     2461 agtgccgttg atcgtgctat gatcgactga tgtcatcagc ggtggagtgc aatgtcgtgc
     2521 aatacgaatg gcgaaaagcc gagctcatcg gtcagcttct caaccttggg gttacccccg
     2581 gcggtgtgct gctggtccac agctccttcc gtagcgtccg gcccctcgaa gatgggccac
     2641 ttggactgat cgaggccctg cgtgctacgc tgggtccggg agggacgctc gtcatgccct
     2701 cgtggtcagg tctggacgac gagccgttcg atcctgccac gtcgcccgtt acaccggacc
     2761 ttggagttgt ctctgacaca ttctggcgcc tgccaaatgt aaagcgcagc gcccatccat
     2821 ttgcctttgc ggcagcgggg ccacaggcag agcagatcat ctctgatcca ttgcccctgc
     2881 caccttactc gcctgcaagc ccggtcgccc gtgtccatga actcgatggg caggtacttc
     2941 tcctcggcgt gggacacgat gccaacacga cgctgcatct tgccgagttg atggcaaagg
     3001 ttccctatgg ggtgccgaga cactgcacca ttcttcagga tggcaagttg gtacgcgtcg
     3061 attatctcga gaatgaccac tgctgtgagc gctttgcctt ggcggacagg tggctcaagg
     3121 agaagagcct tcagaaggaa ggtccagtcg gtcatgcctt tgctcggttg atccgctccc
     3181 gcgacattgt ggcgacagcc ctgggtcaac tgggccgaga tccgttgatc ttcctgcatc
     3241 cgccagaggc gggatgcgaa gaatgcgatg ccgctcgcca gtcgattggc tgagctcatg
     3301 agcggagaac gagatgacgt tggaggggca aggtcgcgct gattgctggg gcaacacggg
     3361 ggatccacta gttccatgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3421 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3481 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3541 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     3601 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3661 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3721 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3781 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aggggagaaa
     3841 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3901 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3961 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4021 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4081 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     4141 ccgaacgacc gagcgcaacg cgtgcggccg cggtacccgg ggatcctcta gagtcgacca
     4201 ccaagggcac catctctgct tgggccaccc cgttggccgc agccagctcg ctgagagccg
     4261 tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct gcaactcccg
     4321 gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg tgtgaatgcc
     4381 cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa agtagccaga
     4441 tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag agttgcagac
     4501 ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga gcgggcgacg
     4561 ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac atatgggccg
     4621 gtcaagatag gtttttaccc cctctcggct gcatcctcta agtggaaaga aattgcaggt
     4681 cgtagaagcg cgttgaagcc tgagagttgc acaggagttg caacccggta gccttgttca
     4741 cgacgagagg agacctagtt ggcacgtcgc ggatggggat cgctgaagac tcagcgcagc
     4801 gggaggatcc aagcctcata cgtcaacccg caggacggtg tgaggtacta cgcgctgcag
     4861 acctacgaca acaagatgga cgccgaagcc tggctcgcgg gcgagaagcg gctcatcgag
     4921 atggagacct ggacccctcc acaggaccgg gcgaagaagg cagccgccag cgccatcacg
     4981 ctggaggagt acacccggaa gtggctcgtg gagcgcgacc tcgcagacgg caccagggat
     5041 ctgtacagcg ggcacgcgga gcgccgcatc tacccggtgc taggtgaagt ggcggtcaca
     5101 gagatgacgc cagctctggt gcgtgcgtgg tgggccggga tgggtaggaa gcacccgact
     5161 gcccgccggc atgcctacaa cgtcctccgg gcggtgatga acacagcggt cgaggacaag
     5221 ctgatcgcag agaacccgtg ccggatcgag cagaaggcag ccgatgagcg cgacgtagag
     5281 gcgctgacgc ctgaggagct ggacatcgtc gccgctgaga tcttcgagca ctaccggatc
     5341 gcggcataca tcctggcgtg gacgagcctc cggttcggag agctgatcga gcttcgccgc
     5401 aaggacatcg tggacgacgg catgacgatg aagctccggg tgcgccgtgg cgcttcccgc
     5461 gtggggaaca agatcgtcgt tggcaacgcc aagaccgtcc ggtcgaagcg tcctgtgacg
     5521 gttccgcctc acgtcgcgga gatgatccga gcgcacatga aggaccgtac gaagatgaac
     5581 aagggccccg aggcattcct ggtgaccacg acgcagggca accggctgtc gaagtccgcg
     5641 ttcaccaagt cgctgaagcg tggctacgcc aagatcggtc ggccggaact ccgcatccac
     5701 gacctccgcg ctgtcggcgc tacgttcgcc gctcaggcag gtgcgacgac caaggagctg
     5761 atggcccgtc tcggtcacac gactcctagg atggcgatga agtaccagat ggcgtctgag
     5821 gcccgcgacg aggctatcgc tgaggcgatg tccaagctgg ccaagacctc ctgaaacgca
     5881 aaaagccccc ctcccaagga cactgagtcc taaagagggg ggtttcttgt cagtacgcga
     5941 agaaccacgc ctggccgcga gcgccagcac cgccgctctg tgcggagacc tgggcaccag
     6001 ccccgccgcc gccaggagca ttgccgttcc cgccagaaat ctagacggtg accacaacgc
     6061 gcccgctttg atcggggacg tcgaaatatt ggatcgtcgg caccgtcacg gccgtgggag
     6121 gcggcacgat ccgcgacgtg atgatcggcc gcatccccac ggtgctgcgc agtgagctct
     6181 acgccatccc ggcgttgatc tgtgcgttcg cacgcacagg cccggtgtga gaagggtctc
     6241 tgacgagcgg gagaactccc tatcag
//
