LOCUS       sequence-315456-        5363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0415:Dendra
FEATURES             Location/Qualifiers
     source          1..5363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1307..1330
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1587..2387
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2512..3100
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3001..3020
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgagcgcaac agatctgagt ccgacctcgc tgcgtgaggc
      121 gttcggacat ttcccgtccg gtgtcatcgc gatcgcggcc gaggtggacg gcacccgggt
      181 cgggctcgcc gccagcacat tcgtcccggt gtccctggag ccgccgctgg tggcgttctg
      241 cgtgcagaac tcctcgacca cgtggcccaa gctcaaggat ctgccgtcgc tcggcatcag
      301 tgtcctcggc gaagcgcacg acaccgccgc ccgcaccctg gccgccaaga ccggtgaccg
      361 attcgccggc ctggagaccg agtcccgcga cagcggcgcg gtgttcatcc acggcaccgc
      421 cgtgtggctg gagagcgcga tcgaacagct ggtcccggcc ggtgaccaca cgatcgtcgt
      481 actgcgggtc agcgacatcg tcgtcaacga cgacgttgcg ccgatcgtgt tccatcgcag
      541 cagcttccgc aagctcggcg ccaagcttgg tgcggccgcg ggcaacacgc ctggcatcaa
      601 tctgatcaag gaggacatgc gtgtcaaggt gcatatggag ggcaacgtga acggtcacgc
      661 cttcgtgatc gagggggagg gtaagggtaa gccttacgaa gggacccaga ccgccaacct
      721 caccgtcaag gagggcgccc ctctcccatt ctcgtacgat atcctcacta ccgcggtgca
      781 ttacgggaac cgcgtgttca ccaagtaccc cgaggatatc cctgactact tcaagcagag
      841 cttcccggag ggatactcct gggagcggac gatgaccttc gaagacaagg gtatctgtac
      901 gatccgcagc gacatctcgc tggaagggga ctgtttcttc cagaacgtcc gtttcaaggg
      961 gaccaacttt ccgccgaacg gcccggtcat gcagaagaag accctcaagt gggagccgag
     1021 cacggagaag ctgcacgttc gggacggcct cctggtcggc aacatcaaca tggcgctgct
     1081 gctggaaggc ggcggccact acctatgcga cttcaagacc acgtacaaag cgaagaaggt
     1141 cgtccagctg cccgacgcgc acttcgtgga ccaccgcatc gaaatcctgg ggaacgattc
     1201 ggactacaac aaggtcaagt tgtacgagca cgctgtggca cgctatagcc cgctcccgtc
     1261 gcaggtctgg ttcgaaggcg gcggcgggga gaacctgtat ttccaggact acaaggacga
     1321 cgacgacaag tgatttaaat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     1381 gttaactagc gtacgatcga attcctgcag ctcacggtaa ctgatgccgt atttgcagta
     1441 ccagcgtacg gcccacagaa tgatgtcacg ctgaaaatgc cggcctttga atgggttcat
     1501 gtgcagctcc atcagcaaaa ggggatgata agtttatcac caccgactat ttgcaacagt
     1561 gccgttgatc gtgctatgat cgactgatgt catcagcggt ggagtgcaat gtcgtgcaat
     1621 acgaatggcg aaaagccgag ctcatcggtc agcttctcaa ccttggggtt acccccggcg
     1681 gtgtgctgct ggtccacagc tccttccgta gcgtccggcc cctcgaagat gggccacttg
     1741 gactgatcga ggccctgcgt gctacgctgg gtccgggagg gacgctcgtc atgccctcgt
     1801 ggtcaggtct ggacgacgag ccgttcgatc ctgccacgtc gcccgttaca ccggaccttg
     1861 gagttgtctc tgacacattc tggcgcctgc caaatgtaaa gcgcagcgcc catccatttg
     1921 cctttgcggc agcggggcca caggcagagc agatcatctc tgatccattg cccctgccac
     1981 cttactcgcc tgcaagcccg gtcgcccgtg tccatgaact cgatgggcag gtacttctcc
     2041 tcggcgtggg acacgatgcc aacacgacgc tgcatcttgc cgagttgatg gcaaaggttc
     2101 cctatggggt gccgagacac tgcaccattc ttcaggatgg caagttggta cgcgtcgatt
     2161 atctcgagaa tgaccactgc tgtgagcgct ttgccttggc ggacaggtgg ctcaaggaga
     2221 agagccttca gaaggaaggt ccagtcggtc atgcctttgc tcggttgatc cgctcccgcg
     2281 acattgtggc gacagccctg ggtcaactgg gccgagatcc gttgatcttc ctgcatccgc
     2341 cagaggcggg atgcgaagaa tgcgatgccg ctcgccagtc gattggctga gctcatgagc
     2401 ggagaacgag atgacgttgg aggggcaagg tcgcgctgat tgctggggca acacggggga
     2461 tccactagtt ccatgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2521 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2581 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2641 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2701 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2761 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2821 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2881 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgagg ggagaaaggc
     2941 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3001 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3061 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3121 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3181 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3241 aacgaccgag cgcaacgcgt gcggccgcgg tacccgggga tcctctagag tcgaccacca
     3301 agggcaccat ctctgcttgg gccaccccgt tggccgcagc cagctcgctg agagccgtga
     3361 acgacagggc gaacgccagc ccgccgacgg cgagggttcc gaccgctgca actcccggtg
     3421 caaccttgtc ccggtctatt ctcttcactg caccagctcc aatctggtgt gaatgcccct
     3481 cgtctgttcg cgcaggcggg gggctctatt cgtttgtcag catcgaaagt agccagatca
     3541 gggatgcgtt gcaaccgcgt atgcccaggt cagaagagtc gcacaagagt tgcagacccc
     3601 tggaaagaaa aatggccaga gggcgaaaac accctctgac cagcggagcg ggcgacggga
     3661 atcgaacccg cgtagctagt ttggaagaat gggtgtctgc cgaccacata tgggccggtc
     3721 aagataggtt tttaccccct ctcggctgca tcctctaagt ggaaagaaat tgcaggtcgt
     3781 agaagcgcgt tgaagcctga gagttgcaca ggagttgcaa cccggtagcc ttgttcacga
     3841 cgagaggaga cctagttggc acgtcgcgga tggggatcgc tgaagactca gcgcagcggg
     3901 aggatccaag cctcatacgt caacccgcag gacggtgtga ggtactacgc gctgcagacc
     3961 tacgacaaca agatggacgc cgaagcctgg ctcgcgggcg agaagcggct catcgagatg
     4021 gagacctgga cccctccaca ggaccgggcg aagaaggcag ccgccagcgc catcacgctg
     4081 gaggagtaca cccggaagtg gctcgtggag cgcgacctcg cagacggcac cagggatctg
     4141 tacagcgggc acgcggagcg ccgcatctac ccggtgctag gtgaagtggc ggtcacagag
     4201 atgacgccag ctctggtgcg tgcgtggtgg gccgggatgg gtaggaagca cccgactgcc
     4261 cgccggcatg cctacaacgt cctccgggcg gtgatgaaca cagcggtcga ggacaagctg
     4321 atcgcagaga acccgtgccg gatcgagcag aaggcagccg atgagcgcga cgtagaggcg
     4381 ctgacgcctg aggagctgga catcgtcgcc gctgagatct tcgagcacta ccggatcgcg
     4441 gcatacatcc tggcgtggac gagcctccgg ttcggagagc tgatcgagct tcgccgcaag
     4501 gacatcgtgg acgacggcat gacgatgaag ctccgggtgc gccgtggcgc ttcccgcgtg
     4561 gggaacaaga tcgtcgttgg caacgccaag accgtccggt cgaagcgtcc tgtgacggtt
     4621 ccgcctcacg tcgcggagat gatccgagcg cacatgaagg accgtacgaa gatgaacaag
     4681 ggccccgagg cattcctggt gaccacgacg cagggcaacc ggctgtcgaa gtccgcgttc
     4741 accaagtcgc tgaagcgtgg ctacgccaag atcggtcggc cggaactccg catccacgac
     4801 ctccgcgctg tcggcgctac gttcgccgct caggcaggtg cgacgaccaa ggagctgatg
     4861 gcccgtctcg gtcacacgac tcctaggatg gcgatgaagt accagatggc gtctgaggcc
     4921 cgcgacgagg ctatcgctga ggcgatgtcc aagctggcca agacctcctg aaacgcaaaa
     4981 agcccccctc ccaaggacac tgagtcctaa agaggggggt ttcttgtcag tacgcgaaga
     5041 accacgcctg gccgcgagcg ccagcaccgc cgctctgtgc ggagacctgg gcaccagccc
     5101 cgccgccgcc aggagcattg ccgttcccgc cagaaatcta gacggtgacc acaacgcgcc
     5161 cgctttgatc ggggacgtcg aaatattgga tcgtcggcac cgtcacggcc gtgggaggcg
     5221 gcacgatccg cgacgtgatg atcggccgca tccccacggt gctgcgcagt gagctctacg
     5281 ccatcccggc gttgatctgt gcgttcgcac gcacaggccc ggtgtgagaa gggtctctga
     5341 cgagcgggag aactccctat cag
//
