LOCUS       sequence-315459-        5237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0713:Dendra
FEATURES             Location/Qualifiers
     source          1..5237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1181..1204
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1461..2261
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2386..2974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2875..2894
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgagcggcaa acgcaacgaa gaagcccgta cgtttctgat
      121 ctctgtggcg gccgagctcg ccggcatgca tgcgcagacc ctgcgcacct atgaccggct
      181 gggcctggtc accccgcagc gcacatccgg tggcgggcgg cgttattcgg aacgcgacgt
      241 cgacctgctg cgagaggtgc agcggctgtc ccaggacgag ggcgtgaacc tggccggcat
      301 caagcgcatc atcgagctga ccaatcaggt cgacgcgctt cagcgccggg tcgccgagct
      361 gaccgccgag gtcgagcgac tgcgcaagcc cagcaccgcg ttggtgctct ggcagccacg
      421 gaacaaccga aaacgcaagc ttggtgcggc cgcgggcaac acgcctggca tcaatctgat
      481 caaggaggac atgcgtgtca aggtgcatat ggagggcaac gtgaacggtc acgccttcgt
      541 gatcgagggg gagggtaagg gtaagcctta cgaagggacc cagaccgcca acctcaccgt
      601 caaggagggc gcccctctcc cattctcgta cgatatcctc actaccgcgg tgcattacgg
      661 gaaccgcgtg ttcaccaagt accccgagga tatccctgac tacttcaagc agagcttccc
      721 ggagggatac tcctgggagc ggacgatgac cttcgaagac aagggtatct gtacgatccg
      781 cagcgacatc tcgctggaag gggactgttt cttccagaac gtccgtttca aggggaccaa
      841 ctttccgccg aacggcccgg tcatgcagaa gaagaccctc aagtgggagc cgagcacgga
      901 gaagctgcac gttcgggacg gcctcctggt cggcaacatc aacatggcgc tgctgctgga
      961 aggcggcggc cactacctat gcgacttcaa gaccacgtac aaagcgaaga aggtcgtcca
     1021 gctgcccgac gcgcacttcg tggaccaccg catcgaaatc ctggggaacg attcggacta
     1081 caacaaggtc aagttgtacg agcacgctgt ggcacgctat agcccgctcc cgtcgcaggt
     1141 ctggttcgaa ggcggcggcg gggagaacct gtatttccag gactacaagg acgacgacga
     1201 caagtgattt aaataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgttaac
     1261 tagcgtacga tcgaattcct gcagctcacg gtaactgatg ccgtatttgc agtaccagcg
     1321 tacggcccac agaatgatgt cacgctgaaa atgccggcct ttgaatgggt tcatgtgcag
     1381 ctccatcagc aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgccgtt
     1441 gatcgtgcta tgatcgactg atgtcatcag cggtggagtg caatgtcgtg caatacgaat
     1501 ggcgaaaagc cgagctcatc ggtcagcttc tcaaccttgg ggttaccccc ggcggtgtgc
     1561 tgctggtcca cagctccttc cgtagcgtcc ggcccctcga agatgggcca cttggactga
     1621 tcgaggccct gcgtgctacg ctgggtccgg gagggacgct cgtcatgccc tcgtggtcag
     1681 gtctggacga cgagccgttc gatcctgcca cgtcgcccgt tacaccggac cttggagttg
     1741 tctctgacac attctggcgc ctgccaaatg taaagcgcag cgcccatcca tttgcctttg
     1801 cggcagcggg gccacaggca gagcagatca tctctgatcc attgcccctg ccaccttact
     1861 cgcctgcaag cccggtcgcc cgtgtccatg aactcgatgg gcaggtactt ctcctcggcg
     1921 tgggacacga tgccaacacg acgctgcatc ttgccgagtt gatggcaaag gttccctatg
     1981 gggtgccgag acactgcacc attcttcagg atggcaagtt ggtacgcgtc gattatctcg
     2041 agaatgacca ctgctgtgag cgctttgcct tggcggacag gtggctcaag gagaagagcc
     2101 ttcagaagga aggtccagtc ggtcatgcct ttgctcggtt gatccgctcc cgcgacattg
     2161 tggcgacagc cctgggtcaa ctgggccgag atccgttgat cttcctgcat ccgccagagg
     2221 cgggatgcga agaatgcgat gccgctcgcc agtcgattgg ctgagctcat gagcggagaa
     2281 cgagatgacg ttggaggggc aaggtcgcgc tgattgctgg ggcaacacgg gggatccact
     2341 agttccatga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2401 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2461 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2521 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2581 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2641 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2701 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2761 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaggggagaa aggcggacag
     2821 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2881 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2941 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3001 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3061 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3121 cgagcgcaac gcgtgcggcc gcggtacccg gggatcctct agagtcgacc accaagggca
     3181 ccatctctgc ttgggccacc ccgttggccg cagccagctc gctgagagcc gtgaacgaca
     3241 gggcgaacgc cagcccgccg acggcgaggg ttccgaccgc tgcaactccc ggtgcaacct
     3301 tgtcccggtc tattctcttc actgcaccag ctccaatctg gtgtgaatgc ccctcgtctg
     3361 ttcgcgcagg cggggggctc tattcgtttg tcagcatcga aagtagccag atcagggatg
     3421 cgttgcaacc gcgtatgccc aggtcagaag agtcgcacaa gagttgcaga cccctggaaa
     3481 gaaaaatggc cagagggcga aaacaccctc tgaccagcgg agcgggcgac gggaatcgaa
     3541 cccgcgtagc tagtttggaa gaatgggtgt ctgccgacca catatgggcc ggtcaagata
     3601 ggtttttacc ccctctcggc tgcatcctct aagtggaaag aaattgcagg tcgtagaagc
     3661 gcgttgaagc ctgagagttg cacaggagtt gcaacccggt agccttgttc acgacgagag
     3721 gagacctagt tggcacgtcg cggatgggga tcgctgaaga ctcagcgcag cgggaggatc
     3781 caagcctcat acgtcaaccc gcaggacggt gtgaggtact acgcgctgca gacctacgac
     3841 aacaagatgg acgccgaagc ctggctcgcg ggcgagaagc ggctcatcga gatggagacc
     3901 tggacccctc cacaggaccg ggcgaagaag gcagccgcca gcgccatcac gctggaggag
     3961 tacacccgga agtggctcgt ggagcgcgac ctcgcagacg gcaccaggga tctgtacagc
     4021 gggcacgcgg agcgccgcat ctacccggtg ctaggtgaag tggcggtcac agagatgacg
     4081 ccagctctgg tgcgtgcgtg gtgggccggg atgggtagga agcacccgac tgcccgccgg
     4141 catgcctaca acgtcctccg ggcggtgatg aacacagcgg tcgaggacaa gctgatcgca
     4201 gagaacccgt gccggatcga gcagaaggca gccgatgagc gcgacgtaga ggcgctgacg
     4261 cctgaggagc tggacatcgt cgccgctgag atcttcgagc actaccggat cgcggcatac
     4321 atcctggcgt ggacgagcct ccggttcgga gagctgatcg agcttcgccg caaggacatc
     4381 gtggacgacg gcatgacgat gaagctccgg gtgcgccgtg gcgcttcccg cgtggggaac
     4441 aagatcgtcg ttggcaacgc caagaccgtc cggtcgaagc gtcctgtgac ggttccgcct
     4501 cacgtcgcgg agatgatccg agcgcacatg aaggaccgta cgaagatgaa caagggcccc
     4561 gaggcattcc tggtgaccac gacgcagggc aaccggctgt cgaagtccgc gttcaccaag
     4621 tcgctgaagc gtggctacgc caagatcggt cggccggaac tccgcatcca cgacctccgc
     4681 gctgtcggcg ctacgttcgc cgctcaggca ggtgcgacga ccaaggagct gatggcccgt
     4741 ctcggtcaca cgactcctag gatggcgatg aagtaccaga tggcgtctga ggcccgcgac
     4801 gaggctatcg ctgaggcgat gtccaagctg gccaagacct cctgaaacgc aaaaagcccc
     4861 cctcccaagg acactgagtc ctaaagaggg gggtttcttg tcagtacgcg aagaaccacg
     4921 cctggccgcg agcgccagca ccgccgctct gtgcggagac ctgggcacca gccccgccgc
     4981 cgccaggagc attgccgttc ccgccagaaa tctagacggt gaccacaacg cgcccgcttt
     5041 gatcggggac gtcgaaatat tggatcgtcg gcaccgtcac ggccgtggga ggcggcacga
     5101 tccgcgacgt gatgatcggc cgcatcccca cggtgctgcg cagtgagctc tacgccatcc
     5161 cggcgttgat ctgtgcgttc gcacgcacag gcccggtgtg agaagggtct ctgacgagcg
     5221 ggagaactcc ctatcag
//
