LOCUS       sequence-31546-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Cp-VPS29
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             710..727
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(780..798)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tgaatatttt gtttacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtt ccgcgtggta gtagttccac agactttgga gatttagtgc
      121 tattgattgg tgatttaaaa attccctacg gagcaaaaga gttgccgtca aattttagag
      181 agctgctggc tactgataaa atcaactacg ttctctgcac aggtaatgta tgctcgcaag
      241 aatacgtgga aatgctgaaa aatattacca aaaatgtata catagttagt ggagacttgg
      301 actctgcaat ttttaatcct gaccctgaaa gtaatggcgt gtttcccgaa tatgtagtag
      361 tacagattgg agaatttaag ataggattga tgcatggaaa ccaggttctt ccatgggacg
      421 atcctggctc tcttgagcaa tggcaaagac gtctagattg cgatatatta gtaactggac
      481 acacacataa attaagggtt tttgagaaaa atggtaaatt gtttctaaac cctggaacag
      541 caacaggggc atttagtgca ttaactccag atgctcctcc atccttcatg ctaatggccc
      601 ttcaaggcaa taaggttgtt ctttatgtgt atgatttaag agatggaaag accaacgtgg
      661 ctatgagtga attctcaaaa tgatgacgaa gcttgcggcc gcactcgagc accaccacca
      721 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      781 cgctgagcaa taactagcat
//
