LOCUS       sequence-315460-        5162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0026:Dendra
FEATURES             Location/Qualifiers
     source          1..5162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1106..1129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1386..2186
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2311..2899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2800..2819
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgcccaagtc caaagtccgc aaaaagaacg acttcacctc
      121 caggccggtg agccggaccc cggtgaaggt gaaggccgga ccgtccagtg tctggttcgt
      181 cgctctgttc atcggcctca tgctgttcgg tctcatctgg ctactggtgt tccagctggc
      241 ggcaaccaac ccgatcgaca ccccgtcgtt cctccagtgg atggcggatc tcggtccgtg
      301 gaactacgcg atcgcctttg ctttcatgat caccggtctg ttgctcacca tgcgctggcg
      361 caagcttggt gcggccgcgg gcaacacgcc tggcatcaat ctgatcaagg aggacatgcg
      421 tgtcaaggtg catatggagg gcaacgtgaa cggtcacgcc ttcgtgatcg agggggaggg
      481 taagggtaag ccttacgaag ggacccagac cgccaacctc accgtcaagg agggcgcccc
      541 tctcccattc tcgtacgata tcctcactac cgcggtgcat tacgggaacc gcgtgttcac
      601 caagtacccc gaggatatcc ctgactactt caagcagagc ttcccggagg gatactcctg
      661 ggagcggacg atgaccttcg aagacaaggg tatctgtacg atccgcagcg acatctcgct
      721 ggaaggggac tgtttcttcc agaacgtccg tttcaagggg accaactttc cgccgaacgg
      781 cccggtcatg cagaagaaga ccctcaagtg ggagccgagc acggagaagc tgcacgttcg
      841 ggacggcctc ctggtcggca acatcaacat ggcgctgctg ctggaaggcg gcggccacta
      901 cctatgcgac ttcaagacca cgtacaaagc gaagaaggtc gtccagctgc ccgacgcgca
      961 cttcgtggac caccgcatcg aaatcctggg gaacgattcg gactacaaca aggtcaagtt
     1021 gtacgagcac gctgtggcac gctatagccc gctcccgtcg caggtctggt tcgaaggcgg
     1081 cggcggggag aacctgtatt tccaggacta caaggacgac gacgacaagt gatttaaata
     1141 accccttggg gcctctaaac gggtcttgag gggttttttg ttaactagcg tacgatcgaa
     1201 ttcctgcagc tcacggtaac tgatgccgta tttgcagtac cagcgtacgg cccacagaat
     1261 gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca tcagcaaaag
     1321 gggatgataa gtttatcacc accgactatt tgcaacagtg ccgttgatcg tgctatgatc
     1381 gactgatgtc atcagcggtg gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc
     1441 tcatcggtca gcttctcaac cttggggtta cccccggcgg tgtgctgctg gtccacagct
     1501 ccttccgtag cgtccggccc ctcgaagatg ggccacttgg actgatcgag gccctgcgtg
     1561 ctacgctggg tccgggaggg acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc
     1621 cgttcgatcc tgccacgtcg cccgttacac cggaccttgg agttgtctct gacacattct
     1681 ggcgcctgcc aaatgtaaag cgcagcgccc atccatttgc ctttgcggca gcggggccac
     1741 aggcagagca gatcatctct gatccattgc ccctgccacc ttactcgcct gcaagcccgg
     1801 tcgcccgtgt ccatgaactc gatgggcagg tacttctcct cggcgtggga cacgatgcca
     1861 acacgacgct gcatcttgcc gagttgatgg caaaggttcc ctatggggtg ccgagacact
     1921 gcaccattct tcaggatggc aagttggtac gcgtcgatta tctcgagaat gaccactgct
     1981 gtgagcgctt tgccttggcg gacaggtggc tcaaggagaa gagccttcag aaggaaggtc
     2041 cagtcggtca tgcctttgct cggttgatcc gctcccgcga cattgtggcg acagccctgg
     2101 gtcaactggg ccgagatccg ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat
     2161 gcgatgccgc tcgccagtcg attggctgag ctcatgagcg gagaacgaga tgacgttgga
     2221 ggggcaaggt cgcgctgatt gctggggcaa cacgggggat ccactagttc catgagcgtc
     2281 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2341 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2401 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2461 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2521 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2581 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2641 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2701 gctatgagaa agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg
     2761 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2821 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2881 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2941 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3001 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg
     3061 cggccgcggt acccggggat cctctagagt cgaccaccaa gggcaccatc tctgcttggg
     3121 ccaccccgtt ggccgcagcc agctcgctga gagccgtgaa cgacagggcg aacgccagcc
     3181 cgccgacggc gagggttccg accgctgcaa ctcccggtgc aaccttgtcc cggtctattc
     3241 tcttcactgc accagctcca atctggtgtg aatgcccctc gtctgttcgc gcaggcgggg
     3301 ggctctattc gtttgtcagc atcgaaagta gccagatcag ggatgcgttg caaccgcgta
     3361 tgcccaggtc agaagagtcg cacaagagtt gcagacccct ggaaagaaaa atggccagag
     3421 ggcgaaaaca ccctctgacc agcggagcgg gcgacgggaa tcgaacccgc gtagctagtt
     3481 tggaagaatg ggtgtctgcc gaccacatat gggccggtca agataggttt ttaccccctc
     3541 tcggctgcat cctctaagtg gaaagaaatt gcaggtcgta gaagcgcgtt gaagcctgag
     3601 agttgcacag gagttgcaac ccggtagcct tgttcacgac gagaggagac ctagttggca
     3661 cgtcgcggat ggggatcgct gaagactcag cgcagcggga ggatccaagc ctcatacgtc
     3721 aacccgcagg acggtgtgag gtactacgcg ctgcagacct acgacaacaa gatggacgcc
     3781 gaagcctggc tcgcgggcga gaagcggctc atcgagatgg agacctggac ccctccacag
     3841 gaccgggcga agaaggcagc cgccagcgcc atcacgctgg aggagtacac ccggaagtgg
     3901 ctcgtggagc gcgacctcgc agacggcacc agggatctgt acagcgggca cgcggagcgc
     3961 cgcatctacc cggtgctagg tgaagtggcg gtcacagaga tgacgccagc tctggtgcgt
     4021 gcgtggtggg ccgggatggg taggaagcac ccgactgccc gccggcatgc ctacaacgtc
     4081 ctccgggcgg tgatgaacac agcggtcgag gacaagctga tcgcagagaa cccgtgccgg
     4141 atcgagcaga aggcagccga tgagcgcgac gtagaggcgc tgacgcctga ggagctggac
     4201 atcgtcgccg ctgagatctt cgagcactac cggatcgcgg catacatcct ggcgtggacg
     4261 agcctccggt tcggagagct gatcgagctt cgccgcaagg acatcgtgga cgacggcatg
     4321 acgatgaagc tccgggtgcg ccgtggcgct tcccgcgtgg ggaacaagat cgtcgttggc
     4381 aacgccaaga ccgtccggtc gaagcgtcct gtgacggttc cgcctcacgt cgcggagatg
     4441 atccgagcgc acatgaagga ccgtacgaag atgaacaagg gccccgaggc attcctggtg
     4501 accacgacgc agggcaaccg gctgtcgaag tccgcgttca ccaagtcgct gaagcgtggc
     4561 tacgccaaga tcggtcggcc ggaactccgc atccacgacc tccgcgctgt cggcgctacg
     4621 ttcgccgctc aggcaggtgc gacgaccaag gagctgatgg cccgtctcgg tcacacgact
     4681 cctaggatgg cgatgaagta ccagatggcg tctgaggccc gcgacgaggc tatcgctgag
     4741 gcgatgtcca agctggccaa gacctcctga aacgcaaaaa gcccccctcc caaggacact
     4801 gagtcctaaa gaggggggtt tcttgtcagt acgcgaagaa ccacgcctgg ccgcgagcgc
     4861 cagcaccgcc gctctgtgcg gagacctggg caccagcccc gccgccgcca ggagcattgc
     4921 cgttcccgcc agaaatctag acggtgacca caacgcgccc gctttgatcg gggacgtcga
     4981 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
     5041 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
     5101 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
     5161 ag
//
