LOCUS       sequence-315461-        6878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0234:Dendra
FEATURES             Location/Qualifiers
     source          1..6878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2822..2845
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3102..3902
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      4027..4615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4516..4535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgacggttca agcaactcgc ggcaccgcgg acgcgaccgg
      121 gaagtcaggc atcgacctga cccatgtcga tccgcggacc cgcccccaag acgacctgtt
      181 cggccacgtc aacgggcgct ggctgaccga atacgagatc cccgcggacc gggccaccga
      241 cggcgcgttc cgcctgctct acgaccgcgc cgaggagcag atccgcgatc tgatcaccga
      301 ggccgccgcc tcaggcgcgg ccgacggcac cgacgagcag cgcatcggcg acctctacgc
      361 gagcttcatg gacacccaga ccatcgccga gcgtggtctg cagccgctgc tcgacgaact
      421 ggcgttgatc gacgcggccc ccgatgccga cgcactggcg gccgtgctgg gcggactgca
      481 gcgcaccggc gtcggcggcg gtgccggcgt gtacgtcgac accgactcga agaactcgac
      541 gcggtacctg ctgcacatgg gacagtccgg catcggcctg cccgacgagt cgtacttccg
      601 cgacgagcag cacgccgaga tcctggccgg ctaccccgtg cacatcgcca agatgttcgg
      661 cctgatctac ggcggcgacc acaccgacac cgccgcgcgc atcgtcgcgc tggagtccaa
      721 gatcgccgcc gcgcactggg acgtggtcaa acgccgcgac gccgacctga cctacaacct
      781 gcgccggttc gccgacctgc ccgccgaggc ccccggcttc gactggtccg ggtgggtcca
      841 gggactcggc accacgccgg acaccgtggc cgagctcgtg gtccgccagc ccgactacct
      901 gaccgcgttc gcggggctgt gggcctcgga ggatctggag gactggaagg cgtgggcgcg
      961 ctggcgcgtc atccacgccc gcgccgggct gctcaccgac gacctggtgg ccgaggattt
     1021 cgcgttctac gggcgcaccc tgtcgggcac cgagcagatc cgcgaccggt ggaagcgcgc
     1081 ggtgtcggtg gtcgagaacc tcatgggtga cgcgctcggc aagctctatg tgcagcggca
     1141 tttcccgccg gaggccaagg cgcgcatgga cgaactggtg gccaacctgc gtgaggccta
     1201 ccgggtcagc atcgacgagc tggactggat gaccccggag accagggcca aggcgctggc
     1261 gaagctggac aagttcacgc cgaagatcgg ttacccggcc cgctggcgcg actactccgc
     1321 ggtggtgatc aagcgcgacg atctgtacgg caactaccgc cggggctaca tcgtgaactc
     1381 cgaccgtgag ctcgccaagc tcggtggccc cgtggaccgc gacgaatggt tcatgacgcc
     1441 gcagaccgtg aatgcctact acaaccccgg catgaacgaa atcgtgttcc ccgcagcgat
     1501 tctgcaaccg ccgttcttcg acgccgaggc cgacgacgcg gccaactacg gtggcatcgg
     1561 cgcggtgatc ggtcacgaga tcgggcacgg cttcgacgat cagggtgcca agtacgacgg
     1621 cgacggcaac ctcgtggact ggtggaccga cgccgaccgc accgaattcg gtgctcgcac
     1681 caaggctttg atcgagcagt acgagcagtt cacgccgcgc gggctcgacc cgtcgcacca
     1741 tgtcaacggc gcgttcaccg tcggcgagaa catcggtgac ctcggcggcc tgtccatcgc
     1801 gctgctcgcc tacaagctct cgctgaaggg caaggaggcc cccgtcatcg acggcctgac
     1861 cggtgtgcag cgcgtgttct tcggctgggc gcaagtgtgg cgcaccaagt cccgtgaggc
     1921 cgaggccatc cggcgtctgg cggtcgatcc gcattcgccg ccggagttcc ggtgcaacgg
     1981 cgtcgtccgc aacatcgacg acttctacga ggcgttcgat gtcaccgaga acgacgagct
     2041 gttcctcgaa cccgagcgtc gggtccgcat ctggaacaag cttggtgcgg ccgcgggcaa
     2101 cacgcctggc atcaatctga tcaaggagga catgcgtgtc aaggtgcata tggagggcaa
     2161 cgtgaacggt cacgccttcg tgatcgaggg ggagggtaag ggtaagcctt acgaagggac
     2221 ccagaccgcc aacctcaccg tcaaggaggg cgcccctctc ccattctcgt acgatatcct
     2281 cactaccgcg gtgcattacg ggaaccgcgt gttcaccaag taccccgagg atatccctga
     2341 ctacttcaag cagagcttcc cggagggata ctcctgggag cggacgatga ccttcgaaga
     2401 caagggtatc tgtacgatcc gcagcgacat ctcgctggaa ggggactgtt tcttccagaa
     2461 cgtccgtttc aaggggacca actttccgcc gaacggcccg gtcatgcaga agaagaccct
     2521 caagtgggag ccgagcacgg agaagctgca cgttcgggac ggcctcctgg tcggcaacat
     2581 caacatggcg ctgctgctgg aaggcggcgg ccactaccta tgcgacttca agaccacgta
     2641 caaagcgaag aaggtcgtcc agctgcccga cgcgcacttc gtggaccacc gcatcgaaat
     2701 cctggggaac gattcggact acaacaaggt caagttgtac gagcacgctg tggcacgcta
     2761 tagcccgctc ccgtcgcagg tctggttcga aggcggcggc ggggagaacc tgtatttcca
     2821 ggactacaag gacgacgacg acaagtgatt taaataaccc cttggggcct ctaaacgggt
     2881 cttgaggggt tttttgttaa ctagcgtacg atcgaattcc tgcagctcac ggtaactgat
     2941 gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa aatgccggcc
     3001 tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt atcaccaccg
     3061 actatttgca acagtgccgt tgatcgtgct atgatcgact gatgtcatca gcggtggagt
     3121 gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg
     3181 gggttacccc cggcggtgtg ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg
     3241 aagatgggcc acttggactg atcgaggccc tgcgtgctac gctgggtccg ggagggacgc
     3301 tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg
     3361 ttacaccgga ccttggagtt gtctctgaca cattctggcg cctgccaaat gtaaagcgca
     3421 gcgcccatcc atttgccttt gcggcagcgg ggccacaggc agagcagatc atctctgatc
     3481 cattgcccct gccaccttac tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg
     3541 ggcaggtact tctcctcggc gtgggacacg atgccaacac gacgctgcat cttgccgagt
     3601 tgatggcaaa ggttccctat ggggtgccga gacactgcac cattcttcag gatggcaagt
     3661 tggtacgcgt cgattatctc gagaatgacc actgctgtga gcgctttgcc ttggcggaca
     3721 ggtggctcaa ggagaagagc cttcagaagg aaggtccagt cggtcatgcc tttgctcggt
     3781 tgatccgctc ccgcgacatt gtggcgacag ccctgggtca actgggccga gatccgttga
     3841 tcttcctgca tccgccagag gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg
     3901 gctgagctca tgagcggaga acgagatgac gttggagggg caaggtcgcg ctgattgctg
     3961 gggcaacacg ggggatccac tagttccatg agcgtcagac cccgtagaaa agatcaaagg
     4021 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4081 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4141 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     4201 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4261 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4321 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4381 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4441 cgaggggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4501 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4561 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4621 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4681 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4741 cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc cgcggtaccc ggggatcctc
     4801 tagagtcgac caccaagggc accatctctg cttgggccac cccgttggcc gcagccagct
     4861 cgctgagagc cgtgaacgac agggcgaacg ccagcccgcc gacggcgagg gttccgaccg
     4921 ctgcaactcc cggtgcaacc ttgtcccggt ctattctctt cactgcacca gctccaatct
     4981 ggtgtgaatg cccctcgtct gttcgcgcag gcggggggct ctattcgttt gtcagcatcg
     5041 aaagtagcca gatcagggat gcgttgcaac cgcgtatgcc caggtcagaa gagtcgcaca
     5101 agagttgcag acccctggaa agaaaaatgg ccagagggcg aaaacaccct ctgaccagcg
     5161 gagcgggcga cgggaatcga acccgcgtag ctagtttgga agaatgggtg tctgccgacc
     5221 acatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa
     5281 gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg
     5341 tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg atcgctgaag
     5401 actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac
     5461 tacgcgctgc agacctacga caacaagatg gacgccgaag cctggctcgc gggcgagaag
     5521 cggctcatcg agatggagac ctggacccct ccacaggacc gggcgaagaa ggcagccgcc
     5581 agcgccatca cgctggagga gtacacccgg aagtggctcg tggagcgcga cctcgcagac
     5641 ggcaccaggg atctgtacag cgggcacgcg gagcgccgca tctacccggt gctaggtgaa
     5701 gtggcggtca cagagatgac gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg
     5761 aagcacccga ctgcccgccg gcatgcctac aacgtcctcc gggcggtgat gaacacagcg
     5821 gtcgaggaca agctgatcgc agagaacccg tgccggatcg agcagaaggc agccgatgag
     5881 cgcgacgtag aggcgctgac gcctgaggag ctggacatcg tcgccgctga gatcttcgag
     5941 cactaccgga tcgcggcata catcctggcg tggacgagcc tccggttcgg agagctgatc
     6001 gagcttcgcc gcaaggacat cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt
     6061 ggcgcttccc gcgtggggaa caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag
     6121 cgtcctgtga cggttccgcc tcacgtcgcg gagatgatcc gagcgcacat gaaggaccgt
     6181 acgaagatga acaagggccc cgaggcattc ctggtgacca cgacgcaggg caaccggctg
     6241 tcgaagtccg cgttcaccaa gtcgctgaag cgtggctacg ccaagatcgg tcggccggaa
     6301 ctccgcatcc acgacctccg cgctgtcggc gctacgttcg ccgctcaggc aggtgcgacg
     6361 accaaggagc tgatggcccg tctcggtcac acgactccta ggatggcgat gaagtaccag
     6421 atggcgtctg aggcccgcga cgaggctatc gctgaggcga tgtccaagct ggccaagacc
     6481 tcctgaaacg caaaaagccc ccctcccaag gacactgagt cctaaagagg ggggtttctt
     6541 gtcagtacgc gaagaaccac gcctggccgc gagcgccagc accgccgctc tgtgcggaga
     6601 cctgggcacc agccccgccg ccgccaggag cattgccgtt cccgccagaa atctagacgg
     6661 tgaccacaac gcgcccgctt tgatcgggga cgtcgaaata ttggatcgtc ggcaccgtca
     6721 cggccgtggg aggcggcacg atccgcgacg tgatgatcgg ccgcatcccc acggtgctgc
     6781 gcagtgagct ctacgccatc ccggcgttga tctgtgcgtt cgcacgcaca ggcccggtgt
     6841 gagaagggtc tctgacgagc gggagaactc cctatcag
//
