LOCUS       sequence-315462-        5762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0435:Dendra
FEATURES             Location/Qualifiers
     source          1..5762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1706..1729
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1986..2786
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2911..3499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3400..3419
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgggcacgac actggaagtg ctgcgcaccg ggccacttgc
      121 gctggtcgag gacctgggca ggccgggact ggcccacatg ggtgtcaccc ggtcgggtgc
      181 cgcagaccgc cgttcccaca cgctggccaa ccgcctcgtg gccaaccccg gtgagagcgc
      241 caccatcgaa gttaccttcg ggggattctc ggcacgcgtg tgtggcggtg acgtggcgat
      301 cgcggtcacc ggcgccgaca ccgatcccgc ggtcaacgga atcccgttcg gcaccaacag
      361 cattcaccac gtccacgacg gccaggtgat ctctctgggc gcaccgcatt ccggactgcg
      421 cagctacctg gcggtgcggg gcggcatcga cgtcaccccg gttctcggct cacgcagcta
      481 cgacgtcatg tcggcgatcg ggccgtcgcc cctgcgacct ggtgacgtgc ttcccgtcgg
      541 cgagcacacc gacgagttcc ccgaactcga tcaggcgccg gtcgccgcga tcgccgaaga
      601 cgtcgtggag ctgcaggtgg ttccggggcc gcgcgacgac tggttcgtcg accccgacat
      661 cctcgtacgc accaactggc tggtcaccaa ccgcagcgac cgggtgggga tgcgtctggt
      721 gggtatgcca ctggagtacc gcaaccccga ccgccagctg cccagcgagg gcgcgacgcg
      781 cggcgcgatc caggtgccgc ccaacggttt tcccgtgatc ttgggtcctg accacccggt
      841 gaccggcgga tacccggtga tcggcgtggt caccgaggag gacatcgaca agctggggca
      901 ggtgcggccg ggtcagaccg tacgactgca ctgggcctac ccccggcgac cgttcgagac
      961 caagcttggt gcggccgcgg gcaacacgcc tggcatcaat ctgatcaagg aggacatgcg
     1021 tgtcaaggtg catatggagg gcaacgtgaa cggtcacgcc ttcgtgatcg agggggaggg
     1081 taagggtaag ccttacgaag ggacccagac cgccaacctc accgtcaagg agggcgcccc
     1141 tctcccattc tcgtacgata tcctcactac cgcggtgcat tacgggaacc gcgtgttcac
     1201 caagtacccc gaggatatcc ctgactactt caagcagagc ttcccggagg gatactcctg
     1261 ggagcggacg atgaccttcg aagacaaggg tatctgtacg atccgcagcg acatctcgct
     1321 ggaaggggac tgtttcttcc agaacgtccg tttcaagggg accaactttc cgccgaacgg
     1381 cccggtcatg cagaagaaga ccctcaagtg ggagccgagc acggagaagc tgcacgttcg
     1441 ggacggcctc ctggtcggca acatcaacat ggcgctgctg ctggaaggcg gcggccacta
     1501 cctatgcgac ttcaagacca cgtacaaagc gaagaaggtc gtccagctgc ccgacgcgca
     1561 cttcgtggac caccgcatcg aaatcctggg gaacgattcg gactacaaca aggtcaagtt
     1621 gtacgagcac gctgtggcac gctatagccc gctcccgtcg caggtctggt tcgaaggcgg
     1681 cggcggggag aacctgtatt tccaggacta caaggacgac gacgacaagt gatttaaata
     1741 accccttggg gcctctaaac gggtcttgag gggttttttg ttaactagcg tacgatcgaa
     1801 ttcctgcagc tcacggtaac tgatgccgta tttgcagtac cagcgtacgg cccacagaat
     1861 gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca tcagcaaaag
     1921 gggatgataa gtttatcacc accgactatt tgcaacagtg ccgttgatcg tgctatgatc
     1981 gactgatgtc atcagcggtg gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc
     2041 tcatcggtca gcttctcaac cttggggtta cccccggcgg tgtgctgctg gtccacagct
     2101 ccttccgtag cgtccggccc ctcgaagatg ggccacttgg actgatcgag gccctgcgtg
     2161 ctacgctggg tccgggaggg acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc
     2221 cgttcgatcc tgccacgtcg cccgttacac cggaccttgg agttgtctct gacacattct
     2281 ggcgcctgcc aaatgtaaag cgcagcgccc atccatttgc ctttgcggca gcggggccac
     2341 aggcagagca gatcatctct gatccattgc ccctgccacc ttactcgcct gcaagcccgg
     2401 tcgcccgtgt ccatgaactc gatgggcagg tacttctcct cggcgtggga cacgatgcca
     2461 acacgacgct gcatcttgcc gagttgatgg caaaggttcc ctatggggtg ccgagacact
     2521 gcaccattct tcaggatggc aagttggtac gcgtcgatta tctcgagaat gaccactgct
     2581 gtgagcgctt tgccttggcg gacaggtggc tcaaggagaa gagccttcag aaggaaggtc
     2641 cagtcggtca tgcctttgct cggttgatcc gctcccgcga cattgtggcg acagccctgg
     2701 gtcaactggg ccgagatccg ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat
     2761 gcgatgccgc tcgccagtcg attggctgag ctcatgagcg gagaacgaga tgacgttgga
     2821 ggggcaaggt cgcgctgatt gctggggcaa cacgggggat ccactagttc catgagcgtc
     2881 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2941 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3001 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     3061 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3121 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3181 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3241 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3301 gctatgagaa agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg
     3361 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3421 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3481 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3541 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3601 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg
     3661 cggccgcggt acccggggat cctctagagt cgaccaccaa gggcaccatc tctgcttggg
     3721 ccaccccgtt ggccgcagcc agctcgctga gagccgtgaa cgacagggcg aacgccagcc
     3781 cgccgacggc gagggttccg accgctgcaa ctcccggtgc aaccttgtcc cggtctattc
     3841 tcttcactgc accagctcca atctggtgtg aatgcccctc gtctgttcgc gcaggcgggg
     3901 ggctctattc gtttgtcagc atcgaaagta gccagatcag ggatgcgttg caaccgcgta
     3961 tgcccaggtc agaagagtcg cacaagagtt gcagacccct ggaaagaaaa atggccagag
     4021 ggcgaaaaca ccctctgacc agcggagcgg gcgacgggaa tcgaacccgc gtagctagtt
     4081 tggaagaatg ggtgtctgcc gaccacatat gggccggtca agataggttt ttaccccctc
     4141 tcggctgcat cctctaagtg gaaagaaatt gcaggtcgta gaagcgcgtt gaagcctgag
     4201 agttgcacag gagttgcaac ccggtagcct tgttcacgac gagaggagac ctagttggca
     4261 cgtcgcggat ggggatcgct gaagactcag cgcagcggga ggatccaagc ctcatacgtc
     4321 aacccgcagg acggtgtgag gtactacgcg ctgcagacct acgacaacaa gatggacgcc
     4381 gaagcctggc tcgcgggcga gaagcggctc atcgagatgg agacctggac ccctccacag
     4441 gaccgggcga agaaggcagc cgccagcgcc atcacgctgg aggagtacac ccggaagtgg
     4501 ctcgtggagc gcgacctcgc agacggcacc agggatctgt acagcgggca cgcggagcgc
     4561 cgcatctacc cggtgctagg tgaagtggcg gtcacagaga tgacgccagc tctggtgcgt
     4621 gcgtggtggg ccgggatggg taggaagcac ccgactgccc gccggcatgc ctacaacgtc
     4681 ctccgggcgg tgatgaacac agcggtcgag gacaagctga tcgcagagaa cccgtgccgg
     4741 atcgagcaga aggcagccga tgagcgcgac gtagaggcgc tgacgcctga ggagctggac
     4801 atcgtcgccg ctgagatctt cgagcactac cggatcgcgg catacatcct ggcgtggacg
     4861 agcctccggt tcggagagct gatcgagctt cgccgcaagg acatcgtgga cgacggcatg
     4921 acgatgaagc tccgggtgcg ccgtggcgct tcccgcgtgg ggaacaagat cgtcgttggc
     4981 aacgccaaga ccgtccggtc gaagcgtcct gtgacggttc cgcctcacgt cgcggagatg
     5041 atccgagcgc acatgaagga ccgtacgaag atgaacaagg gccccgaggc attcctggtg
     5101 accacgacgc agggcaaccg gctgtcgaag tccgcgttca ccaagtcgct gaagcgtggc
     5161 tacgccaaga tcggtcggcc ggaactccgc atccacgacc tccgcgctgt cggcgctacg
     5221 ttcgccgctc aggcaggtgc gacgaccaag gagctgatgg cccgtctcgg tcacacgact
     5281 cctaggatgg cgatgaagta ccagatggcg tctgaggccc gcgacgaggc tatcgctgag
     5341 gcgatgtcca agctggccaa gacctcctga aacgcaaaaa gcccccctcc caaggacact
     5401 gagtcctaaa gaggggggtt tcttgtcagt acgcgaagaa ccacgcctgg ccgcgagcgc
     5461 cagcaccgcc gctctgtgcg gagacctggg caccagcccc gccgccgcca ggagcattgc
     5521 cgttcccgcc agaaatctag acggtgacca caacgcgccc gctttgatcg gggacgtcga
     5581 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
     5641 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
     5701 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
     5761 ag
//
