LOCUS       sequence-315464-        5813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0095:Dendra
FEATURES             Location/Qualifiers
     source          1..5813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1757..1780
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2037..2837
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2962..3550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3451..3470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgagttcgtt gcgtaccgac gacgacacct gggacatcgc
      121 cacgagtgtc ggatccaccg cggtcatggt cgccgcggcg cgcgccggtg agaccgagcg
      181 cgacgacgcc ctgatcaggg atccctacgc caagatcttg gtggccgggg ccggcaccgg
      241 ggtgtgggag acagtgctcg acagcgagtt cgcggccaag atcgagaatc tcgaccccga
      301 ggcggcggcg atcttcgccc acatgggcaa ctaccaggcc gtgcgtacgc acttcttcga
      361 cgcgtactac cgcgaggccg ccgacgcagg catccgccag atcgtcatcc tggcctcggg
      421 cctggactca cgtgcctacc ggctggactg gcccgcaggc accacggtgt acgagatcga
      481 ccagcccaag gtgctcgagt acaagtccgc gacgctgcgc gagcacggca tcgagcccgt
      541 ggcgcagcga cgtgaggtcc ccgtcgatct gcgcttcgac tggcccaccg cgctgcacga
      601 cgccgggttc gacgcgagcc tgccgaccgc gtggctggcc gaaggcctgc tgatgtacct
      661 gcccgccgag gcgcaggacc ggctgttcga gttggtcacc gagttgtcgg cgcccggcag
      721 ccgtatcgcc gtcgagaccg cgggcgtggc cgccaccgat cgccgcgagg ccatgcggga
      781 gcggttcaag aagttcgccg accagctgaa cctcagctct gcgctcgaca tccaggaact
      841 cgtctacgac gacccggacc gcgcggacgt cgcagaatgg ctcgatgcgc acggctggcg
      901 ggcccggggc gttcatgcgc tcgacgagat gcgcaggctc gaccggctcg tcgaactgcc
      961 cgacgacccc gaccgcgccg cgttctcgac gttcgtcacc gctcagaggc tcaagcttgg
     1021 tgcggccgcg ggcaacacgc ctggcatcaa tctgatcaag gaggacatgc gtgtcaaggt
     1081 gcatatggag ggcaacgtga acggtcacgc cttcgtgatc gagggggagg gtaagggtaa
     1141 gccttacgaa gggacccaga ccgccaacct caccgtcaag gagggcgccc ctctcccatt
     1201 ctcgtacgat atcctcacta ccgcggtgca ttacgggaac cgcgtgttca ccaagtaccc
     1261 cgaggatatc cctgactact tcaagcagag cttcccggag ggatactcct gggagcggac
     1321 gatgaccttc gaagacaagg gtatctgtac gatccgcagc gacatctcgc tggaagggga
     1381 ctgtttcttc cagaacgtcc gtttcaaggg gaccaacttt ccgccgaacg gcccggtcat
     1441 gcagaagaag accctcaagt gggagccgag cacggagaag ctgcacgttc gggacggcct
     1501 cctggtcggc aacatcaaca tggcgctgct gctggaaggc ggcggccact acctatgcga
     1561 cttcaagacc acgtacaaag cgaagaaggt cgtccagctg cccgacgcgc acttcgtgga
     1621 ccaccgcatc gaaatcctgg ggaacgattc ggactacaac aaggtcaagt tgtacgagca
     1681 cgctgtggca cgctatagcc cgctcccgtc gcaggtctgg ttcgaaggcg gcggcgggga
     1741 gaacctgtat ttccaggact acaaggacga cgacgacaag tgatttaaat aaccccttgg
     1801 ggcctctaaa cgggtcttga ggggtttttt gttaactagc gtacgatcga attcctgcag
     1861 ctcacggtaa ctgatgccgt atttgcagta ccagcgtacg gcccacagaa tgatgtcacg
     1921 ctgaaaatgc cggcctttga atgggttcat gtgcagctcc atcagcaaaa ggggatgata
     1981 agtttatcac caccgactat ttgcaacagt gccgttgatc gtgctatgat cgactgatgt
     2041 catcagcggt ggagtgcaat gtcgtgcaat acgaatggcg aaaagccgag ctcatcggtc
     2101 agcttctcaa ccttggggtt acccccggcg gtgtgctgct ggtccacagc tccttccgta
     2161 gcgtccggcc cctcgaagat gggccacttg gactgatcga ggccctgcgt gctacgctgg
     2221 gtccgggagg gacgctcgtc atgccctcgt ggtcaggtct ggacgacgag ccgttcgatc
     2281 ctgccacgtc gcccgttaca ccggaccttg gagttgtctc tgacacattc tggcgcctgc
     2341 caaatgtaaa gcgcagcgcc catccatttg cctttgcggc agcggggcca caggcagagc
     2401 agatcatctc tgatccattg cccctgccac cttactcgcc tgcaagcccg gtcgcccgtg
     2461 tccatgaact cgatgggcag gtacttctcc tcggcgtggg acacgatgcc aacacgacgc
     2521 tgcatcttgc cgagttgatg gcaaaggttc cctatggggt gccgagacac tgcaccattc
     2581 ttcaggatgg caagttggta cgcgtcgatt atctcgagaa tgaccactgc tgtgagcgct
     2641 ttgccttggc ggacaggtgg ctcaaggaga agagccttca gaaggaaggt ccagtcggtc
     2701 atgcctttgc tcggttgatc cgctcccgcg acattgtggc gacagccctg ggtcaactgg
     2761 gccgagatcc gttgatcttc ctgcatccgc cagaggcggg atgcgaagaa tgcgatgccg
     2821 ctcgccagtc gattggctga gctcatgagc ggagaacgag atgacgttgg aggggcaagg
     2881 tcgcgctgat tgctggggca acacggggga tccactagtt ccatgagcgt cagaccccgt
     2941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     3121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3361 aagcgccacg cttcccgagg ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3601 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3661 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcaacgcgt gcggccgcgg
     3721 tacccgggga tcctctagag tcgaccacca agggcaccat ctctgcttgg gccaccccgt
     3781 tggccgcagc cagctcgctg agagccgtga acgacagggc gaacgccagc ccgccgacgg
     3841 cgagggttcc gaccgctgca actcccggtg caaccttgtc ccggtctatt ctcttcactg
     3901 caccagctcc aatctggtgt gaatgcccct cgtctgttcg cgcaggcggg gggctctatt
     3961 cgtttgtcag catcgaaagt agccagatca gggatgcgtt gcaaccgcgt atgcccaggt
     4021 cagaagagtc gcacaagagt tgcagacccc tggaaagaaa aatggccaga gggcgaaaac
     4081 accctctgac cagcggagcg ggcgacggga atcgaacccg cgtagctagt ttggaagaat
     4141 gggtgtctgc cgaccacata tgggccggtc aagataggtt tttaccccct ctcggctgca
     4201 tcctctaagt ggaaagaaat tgcaggtcgt agaagcgcgt tgaagcctga gagttgcaca
     4261 ggagttgcaa cccggtagcc ttgttcacga cgagaggaga cctagttggc acgtcgcgga
     4321 tggggatcgc tgaagactca gcgcagcggg aggatccaag cctcatacgt caacccgcag
     4381 gacggtgtga ggtactacgc gctgcagacc tacgacaaca agatggacgc cgaagcctgg
     4441 ctcgcgggcg agaagcggct catcgagatg gagacctgga cccctccaca ggaccgggcg
     4501 aagaaggcag ccgccagcgc catcacgctg gaggagtaca cccggaagtg gctcgtggag
     4561 cgcgacctcg cagacggcac cagggatctg tacagcgggc acgcggagcg ccgcatctac
     4621 ccggtgctag gtgaagtggc ggtcacagag atgacgccag ctctggtgcg tgcgtggtgg
     4681 gccgggatgg gtaggaagca cccgactgcc cgccggcatg cctacaacgt cctccgggcg
     4741 gtgatgaaca cagcggtcga ggacaagctg atcgcagaga acccgtgccg gatcgagcag
     4801 aaggcagccg atgagcgcga cgtagaggcg ctgacgcctg aggagctgga catcgtcgcc
     4861 gctgagatct tcgagcacta ccggatcgcg gcatacatcc tggcgtggac gagcctccgg
     4921 ttcggagagc tgatcgagct tcgccgcaag gacatcgtgg acgacggcat gacgatgaag
     4981 ctccgggtgc gccgtggcgc ttcccgcgtg gggaacaaga tcgtcgttgg caacgccaag
     5041 accgtccggt cgaagcgtcc tgtgacggtt ccgcctcacg tcgcggagat gatccgagcg
     5101 cacatgaagg accgtacgaa gatgaacaag ggccccgagg cattcctggt gaccacgacg
     5161 cagggcaacc ggctgtcgaa gtccgcgttc accaagtcgc tgaagcgtgg ctacgccaag
     5221 atcggtcggc cggaactccg catccacgac ctccgcgctg tcggcgctac gttcgccgct
     5281 caggcaggtg cgacgaccaa ggagctgatg gcccgtctcg gtcacacgac tcctaggatg
     5341 gcgatgaagt accagatggc gtctgaggcc cgcgacgagg ctatcgctga ggcgatgtcc
     5401 aagctggcca agacctcctg aaacgcaaaa agcccccctc ccaaggacac tgagtcctaa
     5461 agaggggggt ttcttgtcag tacgcgaaga accacgcctg gccgcgagcg ccagcaccgc
     5521 cgctctgtgc ggagacctgg gcaccagccc cgccgccgcc aggagcattg ccgttcccgc
     5581 cagaaatcta gacggtgacc acaacgcgcc cgctttgatc ggggacgtcg aaatattgga
     5641 tcgtcggcac cgtcacggcc gtgggaggcg gcacgatccg cgacgtgatg atcggccgca
     5701 tccccacggt gctgcgcagt gagctctacg ccatcccggc gttgatctgt gcgttcgcac
     5761 gcacaggccc ggtgtgagaa gggtctctga cgagcgggag aactccctat cag
//
