LOCUS       sequence-315466-        5658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0007:Dendra
FEATURES             Location/Qualifiers
     source          1..5658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1602..1625
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1882..2682
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2807..3395
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3296..3315
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgagttcacc caacgagccg ggatacccgc gcgcggggga
      121 ccggccgggc gccaccaacg gcacgggtcc gggcggcgac agcggagccc tcagcagcaa
      181 ctcgacgcgg gccacggggc acatcaccga ttccggtgac gtgcccccgt ggcagcgcgg
      241 ggtctcccgc accgctcagc aacctggtca gccgctcggc gacaccgagc agcagccccg
      301 tcccgcgccg aggcccgagc cccgcgagac ccgccaggag ccgcggcctg aacatcgcac
      361 cgaggcctac gcgagcgagt tgccggacct ctcgggtccg gtcccccgct caccgcagcg
      421 caagaccggg gccgacgcgc cccgggcatc ggcgcccacc acccgcatcc aggtggccaa
      481 ccggccgcag ccgtcgggac cggtgcgcgc cagcatgcag atccgccgag tggatccgtg
      541 gaccgtgctg aaggtctcac tggtgctctc ggtcgtgctg ttcttcgtat ggatgatcgc
      601 cgtggcgttc ctgtacttgg tgctcggcgg catgggcgtg tggagcaagc tcaacagcaa
      661 tgtcggcgac ctgctgacga gcgcgagcgg cagttcgggc ggtgagctgg tctccagcgg
      721 cacgatcttc ggcggcgcgg ccctcatcgg cctggtcaac atcgtggttc tcagcgcgat
      781 ggcgacggtc ggcgcgttca tctacaacct cacgacagat ctcgtcgggg gtatcgaagt
      841 gacgttggcg gatcggaaag cttggtgcgg ccgcgggcaa cacgcctggc atcaatctga
      901 tcaaggagga catgcgtgtc aaggtgcata tggagggcaa cgtgaacggt cacgccttcg
      961 tgatcgaggg ggagggtaag ggtaagcctt acgaagggac ccagaccgcc aacctcaccg
     1021 tcaaggaggg cgcccctctc ccattctcgt acgatatcct cactaccgcg gtgcattacg
     1081 ggaaccgcgt gttcaccaag taccccgagg atatccctga ctacttcaag cagagcttcc
     1141 cggagggata ctcctgggag cggacgatga ccttcgaaga caagggtatc tgtacgatcc
     1201 gcagcgacat ctcgctggaa ggggactgtt tcttccagaa cgtccgtttc aaggggacca
     1261 actttccgcc gaacggcccg gtcatgcaga agaagaccct caagtgggag ccgagcacgg
     1321 agaagctgca cgttcgggac ggcctcctgg tcggcaacat caacatggcg ctgctgctgg
     1381 aaggcggcgg ccactaccta tgcgacttca agaccacgta caaagcgaag aaggtcgtcc
     1441 agctgcccga cgcgcacttc gtggaccacc gcatcgaaat cctggggaac gattcggact
     1501 acaacaaggt caagttgtac gagcacgctg tggcacgcta tagcccgctc ccgtcgcagg
     1561 tctggttcga aggcggcggc ggggagaacc tgtatttcca ggactacaag gacgacgacg
     1621 acaagtgatt taaataaccc cttggggcct ctaaacgggt cttgaggggt tttttgttaa
     1681 ctagcgtacg atcgaattcc tgcagctcac ggtaactgat gccgtatttg cagtaccagc
     1741 gtacggccca cagaatgatg tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca
     1801 gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt
     1861 tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa
     1921 tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg
     1981 ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg
     2041 atcgaggccc tgcgtgctac gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca
     2101 ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt
     2161 gtctctgaca cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt
     2221 gcggcagcgg ggccacaggc agagcagatc atctctgatc cattgcccct gccaccttac
     2281 tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc
     2341 gtgggacacg atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat
     2401 ggggtgccga gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc
     2461 gagaatgacc actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc
     2521 cttcagaagg aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt
     2581 gtggcgacag ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag
     2641 gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga
     2701 acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg ggggatccac
     2761 tagttccatg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2821 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2881 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2941 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3001 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3061 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3121 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3181 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaggggaga aaggcggaca
     3241 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3301 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3361 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3421 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3481 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3541 ccgagcgcaa cgcgtgcggc cgcggtaccc ggggatcctc tagagtcgac caccaagggc
     3601 accatctctg cttgggccac cccgttggcc gcagccagct cgctgagagc cgtgaacgac
     3661 agggcgaacg ccagcccgcc gacggcgagg gttccgaccg ctgcaactcc cggtgcaacc
     3721 ttgtcccggt ctattctctt cactgcacca gctccaatct ggtgtgaatg cccctcgtct
     3781 gttcgcgcag gcggggggct ctattcgttt gtcagcatcg aaagtagcca gatcagggat
     3841 gcgttgcaac cgcgtatgcc caggtcagaa gagtcgcaca agagttgcag acccctggaa
     3901 agaaaaatgg ccagagggcg aaaacaccct ctgaccagcg gagcgggcga cgggaatcga
     3961 acccgcgtag ctagtttgga agaatgggtg tctgccgacc acatatgggc cggtcaagat
     4021 aggtttttac cccctctcgg ctgcatcctc taagtggaaa gaaattgcag gtcgtagaag
     4081 cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg tagccttgtt cacgacgaga
     4141 ggagacctag ttggcacgtc gcggatgggg atcgctgaag actcagcgca gcgggaggat
     4201 ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac tacgcgctgc agacctacga
     4261 caacaagatg gacgccgaag cctggctcgc gggcgagaag cggctcatcg agatggagac
     4321 ctggacccct ccacaggacc gggcgaagaa ggcagccgcc agcgccatca cgctggagga
     4381 gtacacccgg aagtggctcg tggagcgcga cctcgcagac ggcaccaggg atctgtacag
     4441 cgggcacgcg gagcgccgca tctacccggt gctaggtgaa gtggcggtca cagagatgac
     4501 gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg aagcacccga ctgcccgccg
     4561 gcatgcctac aacgtcctcc gggcggtgat gaacacagcg gtcgaggaca agctgatcgc
     4621 agagaacccg tgccggatcg agcagaaggc agccgatgag cgcgacgtag aggcgctgac
     4681 gcctgaggag ctggacatcg tcgccgctga gatcttcgag cactaccgga tcgcggcata
     4741 catcctggcg tggacgagcc tccggttcgg agagctgatc gagcttcgcc gcaaggacat
     4801 cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt ggcgcttccc gcgtggggaa
     4861 caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag cgtcctgtga cggttccgcc
     4921 tcacgtcgcg gagatgatcc gagcgcacat gaaggaccgt acgaagatga acaagggccc
     4981 cgaggcattc ctggtgacca cgacgcaggg caaccggctg tcgaagtccg cgttcaccaa
     5041 gtcgctgaag cgtggctacg ccaagatcgg tcggccggaa ctccgcatcc acgacctccg
     5101 cgctgtcggc gctacgttcg ccgctcaggc aggtgcgacg accaaggagc tgatggcccg
     5161 tctcggtcac acgactccta ggatggcgat gaagtaccag atggcgtctg aggcccgcga
     5221 cgaggctatc gctgaggcga tgtccaagct ggccaagacc tcctgaaacg caaaaagccc
     5281 ccctcccaag gacactgagt cctaaagagg ggggtttctt gtcagtacgc gaagaaccac
     5341 gcctggccgc gagcgccagc accgccgctc tgtgcggaga cctgggcacc agccccgccg
     5401 ccgccaggag cattgccgtt cccgccagaa atctagacgg tgaccacaac gcgcccgctt
     5461 tgatcgggga cgtcgaaata ttggatcgtc ggcaccgtca cggccgtggg aggcggcacg
     5521 atccgcgacg tgatgatcgg ccgcatcccc acggtgctgc gcagtgagct ctacgccatc
     5581 ccggcgttga tctgtgcgtt cgcacgcaca ggcccggtgt gagaagggtc tctgacgagc
     5641 gggagaactc cctatcag
//
