LOCUS       sequence-315470-        5642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0310:Dendra
FEATURES             Location/Qualifiers
     source          1..5642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1586..1609
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1866..2666
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2791..3379
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3280..3299
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgaatcccac cgaccgcttc gccggccggg cgacgctccg
      121 ccggtcggtg cgcctgctgt cccagttccg cttcgagcag tccgaaccgg cccggttcta
      181 cggcgccctg gccgacgaca cggtcgccat ggtcgccgac ctctggaccg gcgtgacggg
      241 tgattccccc gcaggtcgca cggtgctcga cgtgggcggc ggacccggtt acttcgcgtc
      301 ggcgttcgag caggccggta tgcgctatgt gggcgtcgaa cccgatccgc gcgagatgca
      361 cgcaggtccg gcgggtgaac cgggcgccgg ggtgttcgtc cgggcgtccg gcatggcgtt
      421 gccgttcgcc gactccagcg tcgacatctg cctgtcgtcg aacgtcgccg agcatgtgcg
      481 caacccgtgg cgactgggca acgagatgct gcgggtgacc cgtcccggcg ggctcgcggt
      541 gctgtcgtac accgtgtggc tggggccgtt cggcggtcac gagatgggcc tgacgcacta
      601 tctcggcggc gcacgggccg ccgaacgcta cacgcgcagg cacggtcacc ggccgaagaa
      661 tgactacgga tcgtcgttgt tcgcggtgtc ggcggccgac ggcttgcagt gggcgcgctc
      721 cacgggcgca ctggtggccg cattcccccg ctaccaccca cgatgggcct ggcggatgac
      781 ctcgataccg ggtttccgcg aattcgcggt gagcaacctg gtgctggttc tgtcgccccg
      841 gaagcttggt gcggccgcgg gcaacacgcc tggcatcaat ctgatcaagg aggacatgcg
      901 tgtcaaggtg catatggagg gcaacgtgaa cggtcacgcc ttcgtgatcg agggggaggg
      961 taagggtaag ccttacgaag ggacccagac cgccaacctc accgtcaagg agggcgcccc
     1021 tctcccattc tcgtacgata tcctcactac cgcggtgcat tacgggaacc gcgtgttcac
     1081 caagtacccc gaggatatcc ctgactactt caagcagagc ttcccggagg gatactcctg
     1141 ggagcggacg atgaccttcg aagacaaggg tatctgtacg atccgcagcg acatctcgct
     1201 ggaaggggac tgtttcttcc agaacgtccg tttcaagggg accaactttc cgccgaacgg
     1261 cccggtcatg cagaagaaga ccctcaagtg ggagccgagc acggagaagc tgcacgttcg
     1321 ggacggcctc ctggtcggca acatcaacat ggcgctgctg ctggaaggcg gcggccacta
     1381 cctatgcgac ttcaagacca cgtacaaagc gaagaaggtc gtccagctgc ccgacgcgca
     1441 cttcgtggac caccgcatcg aaatcctggg gaacgattcg gactacaaca aggtcaagtt
     1501 gtacgagcac gctgtggcac gctatagccc gctcccgtcg caggtctggt tcgaaggcgg
     1561 cggcggggag aacctgtatt tccaggacta caaggacgac gacgacaagt gatttaaata
     1621 accccttggg gcctctaaac gggtcttgag gggttttttg ttaactagcg tacgatcgaa
     1681 ttcctgcagc tcacggtaac tgatgccgta tttgcagtac cagcgtacgg cccacagaat
     1741 gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca tcagcaaaag
     1801 gggatgataa gtttatcacc accgactatt tgcaacagtg ccgttgatcg tgctatgatc
     1861 gactgatgtc atcagcggtg gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc
     1921 tcatcggtca gcttctcaac cttggggtta cccccggcgg tgtgctgctg gtccacagct
     1981 ccttccgtag cgtccggccc ctcgaagatg ggccacttgg actgatcgag gccctgcgtg
     2041 ctacgctggg tccgggaggg acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc
     2101 cgttcgatcc tgccacgtcg cccgttacac cggaccttgg agttgtctct gacacattct
     2161 ggcgcctgcc aaatgtaaag cgcagcgccc atccatttgc ctttgcggca gcggggccac
     2221 aggcagagca gatcatctct gatccattgc ccctgccacc ttactcgcct gcaagcccgg
     2281 tcgcccgtgt ccatgaactc gatgggcagg tacttctcct cggcgtggga cacgatgcca
     2341 acacgacgct gcatcttgcc gagttgatgg caaaggttcc ctatggggtg ccgagacact
     2401 gcaccattct tcaggatggc aagttggtac gcgtcgatta tctcgagaat gaccactgct
     2461 gtgagcgctt tgccttggcg gacaggtggc tcaaggagaa gagccttcag aaggaaggtc
     2521 cagtcggtca tgcctttgct cggttgatcc gctcccgcga cattgtggcg acagccctgg
     2581 gtcaactggg ccgagatccg ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat
     2641 gcgatgccgc tcgccagtcg attggctgag ctcatgagcg gagaacgaga tgacgttgga
     2701 ggggcaaggt cgcgctgatt gctggggcaa cacgggggat ccactagttc catgagcgtc
     2761 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2821 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2881 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2941 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3001 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3061 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3121 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3181 gctatgagaa agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg
     3241 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3301 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3361 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3421 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3481 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg
     3541 cggccgcggt acccggggat cctctagagt cgaccaccaa gggcaccatc tctgcttggg
     3601 ccaccccgtt ggccgcagcc agctcgctga gagccgtgaa cgacagggcg aacgccagcc
     3661 cgccgacggc gagggttccg accgctgcaa ctcccggtgc aaccttgtcc cggtctattc
     3721 tcttcactgc accagctcca atctggtgtg aatgcccctc gtctgttcgc gcaggcgggg
     3781 ggctctattc gtttgtcagc atcgaaagta gccagatcag ggatgcgttg caaccgcgta
     3841 tgcccaggtc agaagagtcg cacaagagtt gcagacccct ggaaagaaaa atggccagag
     3901 ggcgaaaaca ccctctgacc agcggagcgg gcgacgggaa tcgaacccgc gtagctagtt
     3961 tggaagaatg ggtgtctgcc gaccacatat gggccggtca agataggttt ttaccccctc
     4021 tcggctgcat cctctaagtg gaaagaaatt gcaggtcgta gaagcgcgtt gaagcctgag
     4081 agttgcacag gagttgcaac ccggtagcct tgttcacgac gagaggagac ctagttggca
     4141 cgtcgcggat ggggatcgct gaagactcag cgcagcggga ggatccaagc ctcatacgtc
     4201 aacccgcagg acggtgtgag gtactacgcg ctgcagacct acgacaacaa gatggacgcc
     4261 gaagcctggc tcgcgggcga gaagcggctc atcgagatgg agacctggac ccctccacag
     4321 gaccgggcga agaaggcagc cgccagcgcc atcacgctgg aggagtacac ccggaagtgg
     4381 ctcgtggagc gcgacctcgc agacggcacc agggatctgt acagcgggca cgcggagcgc
     4441 cgcatctacc cggtgctagg tgaagtggcg gtcacagaga tgacgccagc tctggtgcgt
     4501 gcgtggtggg ccgggatggg taggaagcac ccgactgccc gccggcatgc ctacaacgtc
     4561 ctccgggcgg tgatgaacac agcggtcgag gacaagctga tcgcagagaa cccgtgccgg
     4621 atcgagcaga aggcagccga tgagcgcgac gtagaggcgc tgacgcctga ggagctggac
     4681 atcgtcgccg ctgagatctt cgagcactac cggatcgcgg catacatcct ggcgtggacg
     4741 agcctccggt tcggagagct gatcgagctt cgccgcaagg acatcgtgga cgacggcatg
     4801 acgatgaagc tccgggtgcg ccgtggcgct tcccgcgtgg ggaacaagat cgtcgttggc
     4861 aacgccaaga ccgtccggtc gaagcgtcct gtgacggttc cgcctcacgt cgcggagatg
     4921 atccgagcgc acatgaagga ccgtacgaag atgaacaagg gccccgaggc attcctggtg
     4981 accacgacgc agggcaaccg gctgtcgaag tccgcgttca ccaagtcgct gaagcgtggc
     5041 tacgccaaga tcggtcggcc ggaactccgc atccacgacc tccgcgctgt cggcgctacg
     5101 ttcgccgctc aggcaggtgc gacgaccaag gagctgatgg cccgtctcgg tcacacgact
     5161 cctaggatgg cgatgaagta ccagatggcg tctgaggccc gcgacgaggc tatcgctgag
     5221 gcgatgtcca agctggccaa gacctcctga aacgcaaaaa gcccccctcc caaggacact
     5281 gagtcctaaa gaggggggtt tcttgtcagt acgcgaagaa ccacgcctgg ccgcgagcgc
     5341 cagcaccgcc gctctgtgcg gagacctggg caccagcccc gccgccgcca ggagcattgc
     5401 cgttcccgcc agaaatctag acggtgacca caacgcgccc gctttgatcg gggacgtcga
     5461 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
     5521 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
     5581 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
     5641 ag
//
