LOCUS       sequence-315475-        7862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0382:Dendra
FEATURES             Location/Qualifiers
     source          1..7862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3806..3829
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4086..4886
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      5011..5599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5500..5519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgagcactga gcaatccggc acggagaaag cccgtcggcc
      121 attctttgcg cgggccgtgc ggaccctttc gcctttcatc atcgtcggtt ggctggcggt
      181 catcctcgtc acgacgctgg cagcggtcgg cggcgactgg tctgcggcca tcccggcact
      241 ggagcgtgtt ggtgagaaaa actctgtatc actgatgccc caggacgcac catcggcgaa
      301 ggccatgaag cacatggggc agaagttcga ggagtccgac tcggacagct tcgccatgat
      361 cgtgctggaa gggcaggaac ccctcggccc ggacgcactc gactactaca ccggcctgat
      421 aagcgaactg cgcaacgact cgaaacatgt tgagcacgtt caggatctgt ggggtgaccg
      481 gctcactgcg tcgagcgcgc agagcgccga cggcaaggct gcctacgtcc agttgaacct
      541 cgcgggtaat cagggcacgc cgttgggcga cgagtcggtt gccgcggtcc gtgatctcgt
      601 cgcgcgaact cctgcgccgg agggtgtcag cgtgtacgtg acgggcgcgg cgccgctagt
      661 atccgacatg cagcacagcg gtaaccgttc gatcctgaag atcaccgcgg tcaccgtcgt
      721 catcatcttc gcgatgctgc tgatggtcta caggtcggtc atcaccgtga tcctgctgct
      781 gatcatggtg ggcttcgaag tcgcagcggc tcgcggcatc gtggcattcc tcggcgccaa
      841 cgaggttttc gtgctgtcca cgttcgccgt gaacatgctc gtcttccttg cgatcgcggc
      901 aggcactgac tacggcatct tcttcttcgg tcgatatcaa gaggcgcgag cggccggcga
      961 ggaccgcgag accgcctact acagcatgta ccgcggcgtc accccggtcg tgatggcgtc
     1021 gggtctgaca atcgccggcg cgatcttctg tctgactttc acacgcctgc cgtacttcca
     1081 caccatgggt gtcccgtgtg cgatcgggat gctcgcggcc gtcgcggtcg cggtgaccct
     1141 ggttccggcc ggtgtcgctc tggcaggccg aatcggctgg ctggaaccga aaaggaagat
     1201 gcgagttcgc cgatggcgcg gcatcggcac cgcgatcgtg cgctggccgg cccccattct
     1261 cgtcgcatcg ttggctgtcg cactggtcgg actgttggca ttgcctggct acgaaacgag
     1321 ctacaacgac cgggactaca tcccggcgga catcccggcg aatgccggat ttgccgctgc
     1381 cgaccggcat ttctcgcaag cgcggatgac acccgacatc ctcatggtcg aatccgacca
     1441 tgacatgcga aatcccacag attttctggt cctgaacaag atcgcgaaag ccatcttcaa
     1501 ggttcgcggc gtctcccgcg tacaggggat aacccgcccg gaggggacgc cgatcgagcg
     1561 tacgtcgatc ccatttctgc tgagcctgca gagtgcggga caggtgcagg cgacgaactt
     1621 ccagaagcag cgcatcggag acatgctcaa gcaggtcgac gacatgaaca tgatgatcgg
     1681 cgtcatgaag acgacctatg gcgtcttgca ggagctcgct gccacgaccc attctctgaa
     1741 cgggtcgaca catgaattac gggatgtcgc cagggatctg cgcggcagca tcgcgatctt
     1801 cgacgatctt ctccgtccca tccggaacta tttctactgg gaacctcact gctataacat
     1861 cccgatctgc tggtcgctgc gctccatttt cgatgccatg gacggggtcg accggttgaa
     1921 cgacgaactg caggtgctcg tcgacaacat ggatcggctg gacgccctgg tgccggaact
     1981 ggtggcgcag ttcccggagt acatcgccag catggaaacg ttccgggagc tgaccctcac
     2041 ctcgtacagc acgttctccg gtttgctcca gcagatggac gagcaaacag aggatgtcac
     2101 cgccatggga caggcatttg acgccgccaa gaacgacgac accttcttcc tgcctccaga
     2161 ggtcttcgac aacccggact tcaagcgcgc gatgacttct ttcctgtcac ccgacggaaa
     2221 ggctgctcgc ttcatcattt cgcacaacgg tgaccccgcg acgccggagg gcatcagccg
     2281 cgtcgaccag atgcagaccg ccgcggtaga ggccctcaag ggcacgcctc tgacagacgc
     2341 gcaggtgtac ttggcgggga ccgcgccgac ggcgaaggac tggaaagacg gttccaccta
     2401 cgatctcctg atcgcgggca tcgccgcgat gtgcctgatc ttcatcatca tgttgatcgt
     2461 cacgcgaagc ttcatcgcgg ccttggtcat cgtgggcacc gtcgtgctct ccctgggggc
     2521 atcattcggg ctgtcggtgc tgctgtggca gtacatcctg ggaatccatc tgcactggat
     2581 ggttctcgcg atgtcggtca tcatcctgtt ggccgtcggc tcggattaca acctgctact
     2641 ggtgtcacgc atcaaagaag aagtgggagc cggcatcaac accggcatcg tccgcgccat
     2701 gggtggtacg ggcaaggtgg tgacgtcggc cggcctggtg ttcgcgggca caatggcggc
     2761 gatggtggtc agcgatctgc ggatcatcgg acagatcggt accacgatcg gtttggggct
     2821 gctgttcgac accatgatcg tgcggtcgtt catgacacct tcgatcgccg cgctcctggg
     2881 ccggtggttc tggtggccgc agaaggtgcg gccgcgtcct gcaagtgcgc tcctggagcc
     2941 ctatgggccg cgaccgctgg tgcgcgcatt gctcgaaggt ccgtcgggtg cagcgagccc
     3001 cggtggtccg ggagggcgac tgtccgacga cgacacgccg accggtccga tgcgcacccg
     3061 aaagcttggt gcggccgcgg gcaacacgcc tggcatcaat ctgatcaagg aggacatgcg
     3121 tgtcaaggtg catatggagg gcaacgtgaa cggtcacgcc ttcgtgatcg agggggaggg
     3181 taagggtaag ccttacgaag ggacccagac cgccaacctc accgtcaagg agggcgcccc
     3241 tctcccattc tcgtacgata tcctcactac cgcggtgcat tacgggaacc gcgtgttcac
     3301 caagtacccc gaggatatcc ctgactactt caagcagagc ttcccggagg gatactcctg
     3361 ggagcggacg atgaccttcg aagacaaggg tatctgtacg atccgcagcg acatctcgct
     3421 ggaaggggac tgtttcttcc agaacgtccg tttcaagggg accaactttc cgccgaacgg
     3481 cccggtcatg cagaagaaga ccctcaagtg ggagccgagc acggagaagc tgcacgttcg
     3541 ggacggcctc ctggtcggca acatcaacat ggcgctgctg ctggaaggcg gcggccacta
     3601 cctatgcgac ttcaagacca cgtacaaagc gaagaaggtc gtccagctgc ccgacgcgca
     3661 cttcgtggac caccgcatcg aaatcctggg gaacgattcg gactacaaca aggtcaagtt
     3721 gtacgagcac gctgtggcac gctatagccc gctcccgtcg caggtctggt tcgaaggcgg
     3781 cggcggggag aacctgtatt tccaggacta caaggacgac gacgacaagt gatttaaata
     3841 accccttggg gcctctaaac gggtcttgag gggttttttg ttaactagcg tacgatcgaa
     3901 ttcctgcagc tcacggtaac tgatgccgta tttgcagtac cagcgtacgg cccacagaat
     3961 gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca tcagcaaaag
     4021 gggatgataa gtttatcacc accgactatt tgcaacagtg ccgttgatcg tgctatgatc
     4081 gactgatgtc atcagcggtg gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc
     4141 tcatcggtca gcttctcaac cttggggtta cccccggcgg tgtgctgctg gtccacagct
     4201 ccttccgtag cgtccggccc ctcgaagatg ggccacttgg actgatcgag gccctgcgtg
     4261 ctacgctggg tccgggaggg acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc
     4321 cgttcgatcc tgccacgtcg cccgttacac cggaccttgg agttgtctct gacacattct
     4381 ggcgcctgcc aaatgtaaag cgcagcgccc atccatttgc ctttgcggca gcggggccac
     4441 aggcagagca gatcatctct gatccattgc ccctgccacc ttactcgcct gcaagcccgg
     4501 tcgcccgtgt ccatgaactc gatgggcagg tacttctcct cggcgtggga cacgatgcca
     4561 acacgacgct gcatcttgcc gagttgatgg caaaggttcc ctatggggtg ccgagacact
     4621 gcaccattct tcaggatggc aagttggtac gcgtcgatta tctcgagaat gaccactgct
     4681 gtgagcgctt tgccttggcg gacaggtggc tcaaggagaa gagccttcag aaggaaggtc
     4741 cagtcggtca tgcctttgct cggttgatcc gctcccgcga cattgtggcg acagccctgg
     4801 gtcaactggg ccgagatccg ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat
     4861 gcgatgccgc tcgccagtcg attggctgag ctcatgagcg gagaacgaga tgacgttgga
     4921 ggggcaaggt cgcgctgatt gctggggcaa cacgggggat ccactagttc catgagcgtc
     4981 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5041 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5101 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5161 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5221 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5281 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5341 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5401 gctatgagaa agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg
     5461 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5521 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5581 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5641 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     5701 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg
     5761 cggccgcggt acccggggat cctctagagt cgaccaccaa gggcaccatc tctgcttggg
     5821 ccaccccgtt ggccgcagcc agctcgctga gagccgtgaa cgacagggcg aacgccagcc
     5881 cgccgacggc gagggttccg accgctgcaa ctcccggtgc aaccttgtcc cggtctattc
     5941 tcttcactgc accagctcca atctggtgtg aatgcccctc gtctgttcgc gcaggcgggg
     6001 ggctctattc gtttgtcagc atcgaaagta gccagatcag ggatgcgttg caaccgcgta
     6061 tgcccaggtc agaagagtcg cacaagagtt gcagacccct ggaaagaaaa atggccagag
     6121 ggcgaaaaca ccctctgacc agcggagcgg gcgacgggaa tcgaacccgc gtagctagtt
     6181 tggaagaatg ggtgtctgcc gaccacatat gggccggtca agataggttt ttaccccctc
     6241 tcggctgcat cctctaagtg gaaagaaatt gcaggtcgta gaagcgcgtt gaagcctgag
     6301 agttgcacag gagttgcaac ccggtagcct tgttcacgac gagaggagac ctagttggca
     6361 cgtcgcggat ggggatcgct gaagactcag cgcagcggga ggatccaagc ctcatacgtc
     6421 aacccgcagg acggtgtgag gtactacgcg ctgcagacct acgacaacaa gatggacgcc
     6481 gaagcctggc tcgcgggcga gaagcggctc atcgagatgg agacctggac ccctccacag
     6541 gaccgggcga agaaggcagc cgccagcgcc atcacgctgg aggagtacac ccggaagtgg
     6601 ctcgtggagc gcgacctcgc agacggcacc agggatctgt acagcgggca cgcggagcgc
     6661 cgcatctacc cggtgctagg tgaagtggcg gtcacagaga tgacgccagc tctggtgcgt
     6721 gcgtggtggg ccgggatggg taggaagcac ccgactgccc gccggcatgc ctacaacgtc
     6781 ctccgggcgg tgatgaacac agcggtcgag gacaagctga tcgcagagaa cccgtgccgg
     6841 atcgagcaga aggcagccga tgagcgcgac gtagaggcgc tgacgcctga ggagctggac
     6901 atcgtcgccg ctgagatctt cgagcactac cggatcgcgg catacatcct ggcgtggacg
     6961 agcctccggt tcggagagct gatcgagctt cgccgcaagg acatcgtgga cgacggcatg
     7021 acgatgaagc tccgggtgcg ccgtggcgct tcccgcgtgg ggaacaagat cgtcgttggc
     7081 aacgccaaga ccgtccggtc gaagcgtcct gtgacggttc cgcctcacgt cgcggagatg
     7141 atccgagcgc acatgaagga ccgtacgaag atgaacaagg gccccgaggc attcctggtg
     7201 accacgacgc agggcaaccg gctgtcgaag tccgcgttca ccaagtcgct gaagcgtggc
     7261 tacgccaaga tcggtcggcc ggaactccgc atccacgacc tccgcgctgt cggcgctacg
     7321 ttcgccgctc aggcaggtgc gacgaccaag gagctgatgg cccgtctcgg tcacacgact
     7381 cctaggatgg cgatgaagta ccagatggcg tctgaggccc gcgacgaggc tatcgctgag
     7441 gcgatgtcca agctggccaa gacctcctga aacgcaaaaa gcccccctcc caaggacact
     7501 gagtcctaaa gaggggggtt tcttgtcagt acgcgaagaa ccacgcctgg ccgcgagcgc
     7561 cagcaccgcc gctctgtgcg gagacctggg caccagcccc gccgccgcca ggagcattgc
     7621 cgttcccgcc agaaatctag acggtgacca caacgcgccc gctttgatcg gggacgtcga
     7681 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
     7741 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
     7801 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
     7861 ag
//
