LOCUS       sequence-315488-        8353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible overexpression of  human SPIC .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Fazeli PK, Zhang Y, O'Keefe J, Pesaresi T, Lun M, Lawney B, 
            Steinhauser ML
  TITLE     Prolonged fasting drives a program of metabolic inflammation in 
            human adipose tissue.
  JOURNAL   Mol Metab. 2020 Sep 28;42:101082. doi: 10.1016/j.molmet.2020.101082.
  PUBMED    32992039
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Metab."; date: "2020-09-28"; volume: "42:101082. doi"; pages: " 
            10.1016/j.molmet.2020.101082"
COMMENT     Sequence Label: pCW-SPIC
FEATURES             Location/Qualifiers
     source          1..8353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1295..1805
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1724..1742
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1815..2411
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1815..1834)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2311..2331
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2412..2465
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2466..3083,3084..3089,3090..3125,3126..3128,3129..3164,
                     3165..3167,3168..3203,3204..3212)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3252..3272)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3670..3681)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3886..4119
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4191..4312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4228..4247)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4282..4301
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4352..4487
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4487..4558
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4559..4627,4628..5419)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4777..4796)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5590..6178
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6079..6098
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6332..6349
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6466..6487
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6502..6507,6508..6525,6526..6532)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6540..6556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6545..6567
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6564..6580
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6564..6580
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6599..6619
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6601..6619
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6647..6873
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6874..7054
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7101..7226
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7719..7952
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8137..8181
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattgggcc
      541 accatgacgt gtgttgaaca agacaagctg ggtcaagcat ttgaagatgc ttttgaggtt
      601 ctgaggcaac attcaactgg agatcttcag tactcgccag attacagaaa ttacctggct
      661 ttaatcaacc atcgtcctca tgtcaaagga aattccagct gctatggagt gttgcctaca
      721 gaggagcctg tctataattg gagaacggta attaacagtg ctgcggactt ctattttgaa
      781 ggaaatattc atcaatctct gcagaacata actgaaaacc agctggtaca acccactctt
      841 ctccagcaaa aggggggaaa aggcaggaag aagctccgac tgtttgaata ccttcacgaa
      901 tccctgtata atccggagat ggcatcttgt attcagtggg tagataaaac caaaggcatc
      961 tttcagtttg tatcaaaaaa caaagaaaaa cttgccgagc tttgggggaa aagaaaaggc
     1021 aacaggaaga ccatgactta ccagaaaatg gccagggcac tcagaaatta cggaagaagt
     1081 ggggaaatta ccaaaatccg gaggaagctg acttaccagt tcagtgaggc cattctccaa
     1141 agactctctc catcctattt cctggggaaa gagatcttct attcacagtg tgttcaacct
     1201 gatcaagaat atctcagttt aaataactgg aatgcaaatt ataattatac atatgccaat
     1261 taccatgagc taaatcacca tgattgcttg tgacggggtt ggggttgcgc cttttccaag
     1321 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
     1381 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
     1441 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
     1501 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
     1561 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
     1621 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
     1681 cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca
     1741 agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc
     1801 cccagcaatt caccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     1861 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     1921 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     1981 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     2041 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     2101 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     2161 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     2221 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     2281 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     2341 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     2401 agcccggtgc cgaaggtaga ggttctctcc tcacttgtgg tgatgttgaa gaaaaccctg
     2461 gtccaatgtc tagactggac aagagcaaag tcataaacgg agctctggaa ttactcaatg
     2521 gtgtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc
     2581 ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga
     2641 tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc
     2701 tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac ggggctaaag
     2761 tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt
     2821 tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc
     2881 actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag
     2941 agacacctac caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc
     3001 ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga
     3061 aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt gacttagaca
     3121 tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg
     3181 acgattttga ccttgacatg ctccccgggt aactaagtaa ggatcgatcc aagatatcgt
     3241 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3301 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3361 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3421 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3481 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3541 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     3601 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     3661 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3721 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3781 tccccgcctg tttcgcctcg gcgtccggac tagaggtacc tttaagacca atgacttaca
     3841 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     3901 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     3961 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     4021 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     4081 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     4141 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     4201 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4261 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     4321 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4381 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4441 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggccttt caaatatgta
     4501 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4561 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4621 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4681 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4741 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4801 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4861 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4921 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4981 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5041 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5101 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5161 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5221 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5281 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5341 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5401 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5461 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5521 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5581 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5641 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5701 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5761 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5821 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5881 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5941 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6001 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6061 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6121 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6181 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6241 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6301 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6361 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     6421 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     6481 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     6541 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     6601 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     6661 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     6721 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     6781 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     6841 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     6901 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6961 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7021 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     7081 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     7141 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     7201 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     7261 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     7321 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     7381 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     7441 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     7501 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     7561 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     7621 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     7681 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     7741 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     7801 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     7861 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     7921 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     7981 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     8041 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     8101 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     8161 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     8221 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     8281 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     8341 accccgaggg gac
//
