LOCUS       sequence-315490-       11208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A reporter that monitors MYC protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Blake DR, Vaseva AV, Hodge RG, Kline MP, Gilbert TSK, Tyagi V, Huang
            D, Whiten GC, Larson JE, Wang X, Pearce KH, Herring LE, Graves LM, 
            Frye SV, Emanuele MJ, Cox AD, Der CJ
  TITLE     Application of a MYC degradation screen identifies sensitivity to 
            CDK9 inhibitors in KRAS-mutant pancreatic cancer.
  JOURNAL   Sci Signal. 2019 Jul 16;12(590). pii: 12/590/eaav7259. doi: 
            10.1126/scisignal.aav7259.
  PUBMED    31311847
REFERENCE   2  (bases 1 to 11208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2019-07-16"; volume: "12(590). pii: 
            12/590/eaav7259. doi"; pages: " 10.1126/scisignal.aav7259"
COMMENT     Sequence Label: GPS-MYC
FEATURES             Location/Qualifiers
     source          1..11208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2731..2755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2838..2847
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2844..3521
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(2974..2992)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(3024..3044)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3242..3261
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3436..3459
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    join(3564..4138,4139..4141,4142..4150)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3731..3748)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3958..3977
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4151..4153,4154..4156,4157..4867)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4151..4153,4154..4156,4157..4867)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4196..4217)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4457..4476)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4804..4825
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4889..4913
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4931..6250
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     protein_bind    complement(6252..6276)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6341..6360)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6342..6360)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        6370..6869
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6405..6428)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6802..6821
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6890..7489
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6890..6909)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7386..7406
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7598..8186
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7651..7671)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8069..8080)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8261..8494
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8613..8631)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8699..8803
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8804..8872,8873..9664)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9022..9041)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9835..10423
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10324..10343
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10577..10594
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10834..10855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10870..10875,10876..10893,10894..10900)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10908..10924
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10913..10935
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10932..10948
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10932..10948
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10998..11017
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccta gcgctaccgg
     2821 actcagatcc accggtcgcc accatggcct cctccgagga cgtcatcaag gagttcatgc
     2881 gcttcaaggt gcgcatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     2941 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc
     3001 ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc aaggtgtacg
     3061 tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag ggcttcaagt
     3121 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     3181 tgcaggacgg ctccttcatc tacaaggtga agttcatcgg cgtgaacttc ccctccgacg
     3241 gccccgtaat gcagaagaag actatgggct gggaggcctc caccgagcgc ctgtaccccc
     3301 gcgacggcgt gctgaagggc gagatccaca aggccctgaa gctgaaggac ggcggccact
     3361 acctggtgga gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg cccggctact
     3421 actacgtgga ctccaagctg gacatcacct cccacaacga ggactacacc atcgtggagc
     3481 agtacgagcg cgccgagggc cgccaccacc tgttcctgta gcggccaatt ctgcagtcga
     3541 cggtaccgcg ggcccgggat ccgcccctct ccctcccccc cccctaacgt tactggccga
     3601 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     3661 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     3721 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     3781 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     3841 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     3901 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     3961 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     4021 ggatctgatc tggggcctcg gtacacatgc tttacatgtg tttagtcgag gttaaaaaaa
     4081 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat
     4141 ggccacaacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     4201 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     4261 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     4321 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     4381 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     4441 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     4501 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     4561 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     4621 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     4681 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     4741 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     4801 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     4861 gtacaagtcc ggccggactc agatcatcac aagtttgtac aaaaaagcag gcttagaagg
     4921 agatagaacc atgcccctca acgttagctt caccaacagg aactatgacc tcgactacga
     4981 ctcggtgcag ccgtatttct actgcgacga ggaggagaac ttctaccagc agcagcagca
     5041 gagcgagctg cagcccccgg cgcccagcga ggatatctgg aagaaattcg agctgctgcc
     5101 caccccgccc ctgtccccta gccgccgctc cgggctctgc tcgccctcct acgttgcggt
     5161 cacacccttc tcccttcggg gagacaacga cggcggtggc gggagcttct ccacggccga
     5221 ccagctggag atggtgaccg agctgctggg aggagacatg gtgaaccaga gtttcatctg
     5281 cgacccggac gacgagacct tcatcaaaaa catcatcatc caggactgta tgtggagcgg
     5341 cttctcggcc gccgccaagc tcgtctcaga gaagctggcc tcctaccagg ctgcgcgcaa
     5401 agacagcggc agcccgaacc ccgcccgcgg ccacagcgtc tgctccacct ccagcttgta
     5461 cctgcaggat ctgagcgccg ccgcctcaga gtgcatcgac ccctcggtgg tcttccccta
     5521 ccctctcaac gacagcagct cgcccaagtc ctgcgcctcg caagactcca gcgccttctc
     5581 tccgtcctcg gattctctgc tctcctcgac ggagtcctcc ccgcagggca gccccgagcc
     5641 cctggtgctc catgaggaga caccgcccac caccagcagc gactctgagg aggaacaaga
     5701 agatgaggaa gaaatcgatg ttgtttctgt ggaaaagagg caggctcctg gcaaaaggtc
     5761 agagtctgga tcaccttctg ctggaggcca cagcaaacct cctcacagcc cactggtcct
     5821 caagaggtgc cacgtctcca cacatcagca caactacgca gcgcctccct ccactcggaa
     5881 ggactatcct gctgccaaga gggtcaagtt ggacagtgtc agagtcctga gacagatcag
     5941 caacaaccga aaatgcacca gccccaggtc ctcggacacc gaggagaatg tcaagaggcg
     6001 aacacacaac gtcttggagc gccagaggag gaacgagcta aaacggagct tttttgccct
     6061 gcgtgaccag atcccggagt tggaaaacaa tgaaaaggcc cccaaggtag ttatccttaa
     6121 aaaagccaca gcatacatcc tgtccgtcca agcagaggag caaaagctca tttctgaaga
     6181 ggacttgttg cggaaacgac gagaacagtt gaaacacaaa cttgaacagc tacggaactc
     6241 ttgtgcgtag gacccagctt tcttgtacaa agtggtgata attctgcagt cgacggtacc
     6301 gcgggcccgg gatccaccgg atctagataa ctgatcctct ccctatagtg agtcgtatta
     6361 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     6421 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     6481 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     6541 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     6601 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     6661 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     6721 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     6781 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     6841 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta
     6901 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     6961 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     7021 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     7081 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     7141 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     7201 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     7261 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     7321 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     7381 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     7441 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag gatcataatc
     7501 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     7561 aacctgaaac ataaaatgaa tgcaattgat agatcctaat caacctctgg attacaaaat
     7621 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7681 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7741 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7801 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7861 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     7921 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     7981 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     8041 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     8101 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     8161 gatctccctt tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca
     8221 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     8281 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     8341 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8401 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8461 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     8521 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag
     8581 gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     8641 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     8701 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8761 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     8821 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8881 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8941 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     9001 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     9061 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     9121 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     9181 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     9241 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     9301 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     9361 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     9421 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     9481 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     9541 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     9601 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     9661 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     9721 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9781 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9841 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9901 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9961 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    10021 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    10081 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    10141 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    10201 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    10261 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    10321 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    10381 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    10441 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    10501 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
    10561 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
    10621 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt
    10681 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
    10741 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc
    10801 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    10861 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
    10921 acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt
    10981 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    11041 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    11101 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
    11161 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
