LOCUS       sequence-31553-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Pf-DNCL1
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(321..338)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(348..365)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             384..406
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    421..445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(445..464)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(446..464)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(548..567)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        773..849
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(871..890)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cgggcttgta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag
       61 cttcgtcatc atcccgattt aaataataat atagctactt gtccaatata aaaatatata
      121 aaatttttgg tttcgtgagt aacataagat ccaaaatttc ttcctacaac acagtgccaa
      181 gttgggtcat attttctatc aaattctttt ttaatatgtg cagcaatatc tttttcgacg
      241 ttatattttt gtaaggcctg attagcacaa tcaatagcat caatttgcat ttcttctgtc
      301 atatctacat tttttacaac actaccacgc ggaaccaggc cgctgctgtg atgatgatga
      361 tgatggctgc tgcccatggt atatctcctt cttaaagtta aacaaaatta tttctagagg
      421 ggaattgtta tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag
      481 atctcgatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
      541 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
      601 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
      661 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
      721 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat
      781 cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag
      841 ggtggtgaaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgctcta
//
