LOCUS       sequence-315542-        7424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0732:Dendra
FEATURES             Location/Qualifiers
     source          1..7424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3368..3391
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3648..4448
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      4573..5161
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5062..5081
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggactcgtt caacccgacg accaagactc aggcggcgct
      121 gacctcggcg ttgcaggcgg cgagttccgc tggcaacccg gagatccgcc ccgcgcattt
      181 gctgatggca ttgctgaccc agaatgacgg cattgccgca cccctgctgg aggccgtcgg
      241 cgtcgagccc gcgacgatcc gcaccgagac ccagcggttg ctcgaccgtc tgcccagcgc
      301 ctcgggcgcg agcagccagc cccagctcag ccgcgaatcg ctcgcggcga tcaccacggc
      361 ccagaacctg gccaccgaga tggacgacga gtacgtctcg accgagcacc tgatggtcgg
      421 cctggccacg ggtgactccg aggtggccaa gctgctgacc ggacacggcg cgtcgccgca
      481 ggctctgcgt gaggcgttcg tcaaggtgcg cggcagcgcc cgggtgacca gcccggaccc
      541 ggagggcacg tatcaggcgc tggagaagta ctccaccgac ctgaccgcgc gtgcccgcga
      601 gggcaagctc gacccggtga tcgggcgcga caacgagatt cgtcgtgtcg tacaggtgct
      661 gagccgtcgt accaagaaca atccggtgct catcggcgag ccgggcgtcg gtaagacggc
      721 catcgtcgag ggcctcgcgc agcgcatcgt cgccggcgac gtgcccgaaa gcctgcgcga
      781 caagaccgtc atcgcactgg atctcggttc gatggtggcc ggtgcgaagt accgcggtga
      841 gttcgaggag cggctgaagg ccgtgctcga cgacatcaag aactcggccg ggcaggtcat
      901 cacgttcatc gacgagctgc acaccatcgt cggcgccggt gccaccggtg agtcggcgat
      961 ggacgcgggc aacatgatca agccgatgct ggcccgcggc gagctgcggc tggtcggtgc
     1021 caccacgctc gacgagtacc gcaagtacat cgagaaggac gccgcattgg agcgccggtt
     1081 ccagcaggtg ttcgtcggtg aaccctcggt cgaggacacc gtcggcatcc tgcgtggtct
     1141 gaaggaccgc tacgaggtgc accacggcgt gcggatcacc gactcggcgt tggtggccgc
     1201 ggcgacgctc tccgaccggt acatcaccgc gcggttcctt ccggacaagg ccatcgacct
     1261 ggtcgatgag gccgcgtccc ggctgcgcat ggagatcgac tcgcgcccag ttgaaatcga
     1321 cgaggtcgag aggttggtac ggcgcctgga gatcgaggag atggcgcttg agaaagaaga
     1381 ggacgaggcg tccaaggacc ggctggagaa gctgcgcgcc gaactggccg accacaagga
     1441 gaagctgtcc gagctgacca cgcggtggca gaacgagaag aacgccatca acgtcgttcg
     1501 cgagctcaag gagcaactgg acacgttgcg cggcgaggcc gaccgtgccg agcgtgacgg
     1561 tgacctggcc aaggcggccg aactgcggta cggccgaatc ccggaggtgg agaagaagct
     1621 cgacgctgcc cttcccgccg ccgaggcccg cgagaacctg atgctcaagg aggaggtcgg
     1681 ccccgacgac atcgccgagg tggtggaggc gtggaccggt atcccgtccg gccgcatgct
     1741 cgaaggcgag accgccaagc tgctccgcat ggaggaggag ctgggcaagc gtgtcgtcgg
     1801 gcagcgcaag gccgtgcagg ccgtcagcga cgcggtgcgg cgcagccgtg ccggggtggc
     1861 cgaccccaac cggccgacgg gttcgttcat gttcctgggc ccgaccggtg tgggtaagac
     1921 cgagctcgca aaggcgttgg cggagttctt gttcgacgac gagcgtgcga tggtccgcat
     1981 cgacatgagc gagtacggcg agaagcactc ggtggctcgg ctggtcggtg cgcctcccgg
     2041 ctacatcggt tacgaccagg gcggtcagct gaccgaggcg gtgcggcggc gtccgtacac
     2101 ggtgatcctg ttcgacgaga tcgaaaaggc gcacccggac gtgttcgacg tgctgctgca
     2161 ggtgctcgac gagggccggt tgaccgacgg ccagggccgc acggtcgact tccgcaacac
     2221 catcctcatc ctgacgtcca acctgggcgc gggcggtaac gaggaccagg tgatggcggc
     2281 ggtgcgctcg gcgttcaagc ccgagttcat caaccggctg gacgacgtca tcatcttcga
     2341 cgggctcaat cccgaagagc tggtgcagat cgtcgacatc cagttggcgc agctggccaa
     2401 gcgtctggag cagcggcgac tcaccctcga ggtgtcgctg ccggccaaga agtggctggg
     2461 gcagcgcggt ttcgacccgc tgtacggtgc ccgtccgctg cgccggttgg tgcagcaggc
     2521 catcggcgac cagttggcca agatgctgct ggccggcgag gtgcacgacg gcgacatcgt
     2581 gccggtgaac gtcagccccg acggcgagtc cctcgttttg ggcaagcttg gtgcggccgc
     2641 gggcaacacg cctggcatca atctgatcaa ggaggacatg cgtgtcaagg tgcatatgga
     2701 gggcaacgtg aacggtcacg ccttcgtgat cgagggggag ggtaagggta agccttacga
     2761 agggacccag accgccaacc tcaccgtcaa ggagggcgcc cctctcccat tctcgtacga
     2821 tatcctcact accgcggtgc attacgggaa ccgcgtgttc accaagtacc ccgaggatat
     2881 ccctgactac ttcaagcaga gcttcccgga gggatactcc tgggagcgga cgatgacctt
     2941 cgaagacaag ggtatctgta cgatccgcag cgacatctcg ctggaagggg actgtttctt
     3001 ccagaacgtc cgtttcaagg ggaccaactt tccgccgaac ggcccggtca tgcagaagaa
     3061 gaccctcaag tgggagccga gcacggagaa gctgcacgtt cgggacggcc tcctggtcgg
     3121 caacatcaac atggcgctgc tgctggaagg cggcggccac tacctatgcg acttcaagac
     3181 cacgtacaaa gcgaagaagg tcgtccagct gcccgacgcg cacttcgtgg accaccgcat
     3241 cgaaatcctg gggaacgatt cggactacaa caaggtcaag ttgtacgagc acgctgtggc
     3301 acgctatagc ccgctcccgt cgcaggtctg gttcgaaggc ggcggcgggg agaacctgta
     3361 tttccaggac tacaaggacg acgacgacaa gtgatttaaa taaccccttg gggcctctaa
     3421 acgggtcttg aggggttttt tgttaactag cgtacgatcg aattcctgca gctcacggta
     3481 actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg
     3541 ccggcctttg aatgggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca
     3601 ccaccgacta tttgcaacag tgccgttgat cgtgctatga tcgactgatg tcatcagcgg
     3661 tggagtgcaa tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt cagcttctca
     3721 accttggggt tacccccggc ggtgtgctgc tggtccacag ctccttccgt agcgtccggc
     3781 ccctcgaaga tgggccactt ggactgatcg aggccctgcg tgctacgctg ggtccgggag
     3841 ggacgctcgt catgccctcg tggtcaggtc tggacgacga gccgttcgat cctgccacgt
     3901 cgcccgttac accggacctt ggagttgtct ctgacacatt ctggcgcctg ccaaatgtaa
     3961 agcgcagcgc ccatccattt gcctttgcgg cagcggggcc acaggcagag cagatcatct
     4021 ctgatccatt gcccctgcca ccttactcgc ctgcaagccc ggtcgcccgt gtccatgaac
     4081 tcgatgggca ggtacttctc ctcggcgtgg gacacgatgc caacacgacg ctgcatcttg
     4141 ccgagttgat ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt cttcaggatg
     4201 gcaagttggt acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc tttgccttgg
     4261 cggacaggtg gctcaaggag aagagccttc agaaggaagg tccagtcggt catgcctttg
     4321 ctcggttgat ccgctcccgc gacattgtgg cgacagccct gggtcaactg ggccgagatc
     4381 cgttgatctt cctgcatccg ccagaggcgg gatgcgaaga atgcgatgcc gctcgccagt
     4441 cgattggctg agctcatgag cggagaacga gatgacgttg gaggggcaag gtcgcgctga
     4501 ttgctggggc aacacggggg atccactagt tccatgagcg tcagaccccg tagaaaagat
     4561 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4621 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4681 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     4741 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4801 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4861 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4921 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4981 gcttcccgag gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5041 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5101 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5161 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5221 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     5281 tgataccgct cgccgcagcc gaacgaccga gcgcaacgcg tgcggccgcg gtacccgggg
     5341 atcctctaga gtcgaccacc aagggcacca tctctgcttg ggccaccccg ttggccgcag
     5401 ccagctcgct gagagccgtg aacgacaggg cgaacgccag cccgccgacg gcgagggttc
     5461 cgaccgctgc aactcccggt gcaaccttgt cccggtctat tctcttcact gcaccagctc
     5521 caatctggtg tgaatgcccc tcgtctgttc gcgcaggcgg ggggctctat tcgtttgtca
     5581 gcatcgaaag tagccagatc agggatgcgt tgcaaccgcg tatgcccagg tcagaagagt
     5641 cgcacaagag ttgcagaccc ctggaaagaa aaatggccag agggcgaaaa caccctctga
     5701 ccagcggagc gggcgacggg aatcgaaccc gcgtagctag tttggaagaa tgggtgtctg
     5761 ccgaccacat atgggccggt caagataggt ttttaccccc tctcggctgc atcctctaag
     5821 tggaaagaaa ttgcaggtcg tagaagcgcg ttgaagcctg agagttgcac aggagttgca
     5881 acccggtagc cttgttcacg acgagaggag acctagttgg cacgtcgcgg atggggatcg
     5941 ctgaagactc agcgcagcgg gaggatccaa gcctcatacg tcaacccgca ggacggtgtg
     6001 aggtactacg cgctgcagac ctacgacaac aagatggacg ccgaagcctg gctcgcgggc
     6061 gagaagcggc tcatcgagat ggagacctgg acccctccac aggaccgggc gaagaaggca
     6121 gccgccagcg ccatcacgct ggaggagtac acccggaagt ggctcgtgga gcgcgacctc
     6181 gcagacggca ccagggatct gtacagcggg cacgcggagc gccgcatcta cccggtgcta
     6241 ggtgaagtgg cggtcacaga gatgacgcca gctctggtgc gtgcgtggtg ggccgggatg
     6301 ggtaggaagc acccgactgc ccgccggcat gcctacaacg tcctccgggc ggtgatgaac
     6361 acagcggtcg aggacaagct gatcgcagag aacccgtgcc ggatcgagca gaaggcagcc
     6421 gatgagcgcg acgtagaggc gctgacgcct gaggagctgg acatcgtcgc cgctgagatc
     6481 ttcgagcact accggatcgc ggcatacatc ctggcgtgga cgagcctccg gttcggagag
     6541 ctgatcgagc ttcgccgcaa ggacatcgtg gacgacggca tgacgatgaa gctccgggtg
     6601 cgccgtggcg cttcccgcgt ggggaacaag atcgtcgttg gcaacgccaa gaccgtccgg
     6661 tcgaagcgtc ctgtgacggt tccgcctcac gtcgcggaga tgatccgagc gcacatgaag
     6721 gaccgtacga agatgaacaa gggccccgag gcattcctgg tgaccacgac gcagggcaac
     6781 cggctgtcga agtccgcgtt caccaagtcg ctgaagcgtg gctacgccaa gatcggtcgg
     6841 ccggaactcc gcatccacga cctccgcgct gtcggcgcta cgttcgccgc tcaggcaggt
     6901 gcgacgacca aggagctgat ggcccgtctc ggtcacacga ctcctaggat ggcgatgaag
     6961 taccagatgg cgtctgaggc ccgcgacgag gctatcgctg aggcgatgtc caagctggcc
     7021 aagacctcct gaaacgcaaa aagcccccct cccaaggaca ctgagtccta aagagggggg
     7081 tttcttgtca gtacgcgaag aaccacgcct ggccgcgagc gccagcaccg ccgctctgtg
     7141 cggagacctg ggcaccagcc ccgccgccgc caggagcatt gccgttcccg ccagaaatct
     7201 agacggtgac cacaacgcgc ccgctttgat cggggacgtc gaaatattgg atcgtcggca
     7261 ccgtcacggc cgtgggaggc ggcacgatcc gcgacgtgat gatcggccgc atccccacgg
     7321 tgctgcgcag tgagctctac gccatcccgg cgttgatctg tgcgttcgca cgcacaggcc
     7381 cggtgtgaga agggtctctg acgagcggga gaactcccta tcag
//
