LOCUS       sequence-315543-        5345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0034:Dendra
FEATURES             Location/Qualifiers
     source          1..5345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1289..1312
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1569..2369
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2494..3082
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2983..3002
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgcaggggtt agtgctgcag ctgacgcgcg tcggcttcct
      121 gttgttgcta tggctgttca tctggtcggt gctgcgcatc ctgcgcaccg atatctacgc
      181 ccccacgggc gcggtgatgg tgagacgtgg gctggccctt cgcggctcgc tgctgcccaa
      241 ccggcagcgg cgtcatgtgg cgcggcagct cgtggtcacc gagggcgcac tggccggcac
      301 ccgcatcacg ctgggcaacc agccggtgct gatcggccgg gccgacgact cgacgctggt
      361 gctcaccgac gactatgcgt cgacgcgcca cgccagactg tcgccacgag gttctgagtg
      421 gtacgtcgag gacctaggat cgaccaacgg cacttacctt gacagggcga aggtgacaac
      481 agcagtaaag gttcccattg gcgcgccggt gcggatcggc aagacggtaa tcgagctgcg
      541 cccgaagctt ggtgcggccg cgggcaacac gcctggcatc aatctgatca aggaggacat
      601 gcgtgtcaag gtgcatatgg agggcaacgt gaacggtcac gccttcgtga tcgaggggga
      661 gggtaagggt aagccttacg aagggaccca gaccgccaac ctcaccgtca aggagggcgc
      721 ccctctccca ttctcgtacg atatcctcac taccgcggtg cattacggga accgcgtgtt
      781 caccaagtac cccgaggata tccctgacta cttcaagcag agcttcccgg agggatactc
      841 ctgggagcgg acgatgacct tcgaagacaa gggtatctgt acgatccgca gcgacatctc
      901 gctggaaggg gactgtttct tccagaacgt ccgtttcaag gggaccaact ttccgccgaa
      961 cggcccggtc atgcagaaga agaccctcaa gtgggagccg agcacggaga agctgcacgt
     1021 tcgggacggc ctcctggtcg gcaacatcaa catggcgctg ctgctggaag gcggcggcca
     1081 ctacctatgc gacttcaaga ccacgtacaa agcgaagaag gtcgtccagc tgcccgacgc
     1141 gcacttcgtg gaccaccgca tcgaaatcct ggggaacgat tcggactaca acaaggtcaa
     1201 gttgtacgag cacgctgtgg cacgctatag cccgctcccg tcgcaggtct ggttcgaagg
     1261 cggcggcggg gagaacctgt atttccagga ctacaaggac gacgacgaca agtgatttaa
     1321 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgttaacta gcgtacgatc
     1381 gaattcctgc agctcacggt aactgatgcc gtatttgcag taccagcgta cggcccacag
     1441 aatgatgtca cgctgaaaat gccggccttt gaatgggttc atgtgcagct ccatcagcaa
     1501 aaggggatga taagtttatc accaccgact atttgcaaca gtgccgttga tcgtgctatg
     1561 atcgactgat gtcatcagcg gtggagtgca atgtcgtgca atacgaatgg cgaaaagccg
     1621 agctcatcgg tcagcttctc aaccttgggg ttacccccgg cggtgtgctg ctggtccaca
     1681 gctccttccg tagcgtccgg cccctcgaag atgggccact tggactgatc gaggccctgc
     1741 gtgctacgct gggtccggga gggacgctcg tcatgccctc gtggtcaggt ctggacgacg
     1801 agccgttcga tcctgccacg tcgcccgtta caccggacct tggagttgtc tctgacacat
     1861 tctggcgcct gccaaatgta aagcgcagcg cccatccatt tgcctttgcg gcagcggggc
     1921 cacaggcaga gcagatcatc tctgatccat tgcccctgcc accttactcg cctgcaagcc
     1981 cggtcgcccg tgtccatgaa ctcgatgggc aggtacttct cctcggcgtg ggacacgatg
     2041 ccaacacgac gctgcatctt gccgagttga tggcaaaggt tccctatggg gtgccgagac
     2101 actgcaccat tcttcaggat ggcaagttgg tacgcgtcga ttatctcgag aatgaccact
     2161 gctgtgagcg ctttgccttg gcggacaggt ggctcaagga gaagagcctt cagaaggaag
     2221 gtccagtcgg tcatgccttt gctcggttga tccgctcccg cgacattgtg gcgacagccc
     2281 tgggtcaact gggccgagat ccgttgatct tcctgcatcc gccagaggcg ggatgcgaag
     2341 aatgcgatgc cgctcgccag tcgattggct gagctcatga gcggagaacg agatgacgtt
     2401 ggaggggcaa ggtcgcgctg attgctgggg caacacgggg gatccactag ttccatgagc
     2461 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2521 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2581 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2641 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2701 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2761 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2821 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2881 tgagctatga gaaagcgcca cgcttcccga ggggagaaag gcggacaggt atccggtaag
     2941 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3001 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3061 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3121 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3181 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc
     3241 gtgcggccgc ggtacccggg gatcctctag agtcgaccac caagggcacc atctctgctt
     3301 gggccacccc gttggccgca gccagctcgc tgagagccgt gaacgacagg gcgaacgcca
     3361 gcccgccgac ggcgagggtt ccgaccgctg caactcccgg tgcaaccttg tcccggtcta
     3421 ttctcttcac tgcaccagct ccaatctggt gtgaatgccc ctcgtctgtt cgcgcaggcg
     3481 gggggctcta ttcgtttgtc agcatcgaaa gtagccagat cagggatgcg ttgcaaccgc
     3541 gtatgcccag gtcagaagag tcgcacaaga gttgcagacc cctggaaaga aaaatggcca
     3601 gagggcgaaa acaccctctg accagcggag cgggcgacgg gaatcgaacc cgcgtagcta
     3661 gtttggaaga atgggtgtct gccgaccaca tatgggccgg tcaagatagg tttttacccc
     3721 ctctcggctg catcctctaa gtggaaagaa attgcaggtc gtagaagcgc gttgaagcct
     3781 gagagttgca caggagttgc aacccggtag ccttgttcac gacgagagga gacctagttg
     3841 gcacgtcgcg gatggggatc gctgaagact cagcgcagcg ggaggatcca agcctcatac
     3901 gtcaacccgc aggacggtgt gaggtactac gcgctgcaga cctacgacaa caagatggac
     3961 gccgaagcct ggctcgcggg cgagaagcgg ctcatcgaga tggagacctg gacccctcca
     4021 caggaccggg cgaagaaggc agccgccagc gccatcacgc tggaggagta cacccggaag
     4081 tggctcgtgg agcgcgacct cgcagacggc accagggatc tgtacagcgg gcacgcggag
     4141 cgccgcatct acccggtgct aggtgaagtg gcggtcacag agatgacgcc agctctggtg
     4201 cgtgcgtggt gggccgggat gggtaggaag cacccgactg cccgccggca tgcctacaac
     4261 gtcctccggg cggtgatgaa cacagcggtc gaggacaagc tgatcgcaga gaacccgtgc
     4321 cggatcgagc agaaggcagc cgatgagcgc gacgtagagg cgctgacgcc tgaggagctg
     4381 gacatcgtcg ccgctgagat cttcgagcac taccggatcg cggcatacat cctggcgtgg
     4441 acgagcctcc ggttcggaga gctgatcgag cttcgccgca aggacatcgt ggacgacggc
     4501 atgacgatga agctccgggt gcgccgtggc gcttcccgcg tggggaacaa gatcgtcgtt
     4561 ggcaacgcca agaccgtccg gtcgaagcgt cctgtgacgg ttccgcctca cgtcgcggag
     4621 atgatccgag cgcacatgaa ggaccgtacg aagatgaaca agggccccga ggcattcctg
     4681 gtgaccacga cgcagggcaa ccggctgtcg aagtccgcgt tcaccaagtc gctgaagcgt
     4741 ggctacgcca agatcggtcg gccggaactc cgcatccacg acctccgcgc tgtcggcgct
     4801 acgttcgccg ctcaggcagg tgcgacgacc aaggagctga tggcccgtct cggtcacacg
     4861 actcctagga tggcgatgaa gtaccagatg gcgtctgagg cccgcgacga ggctatcgct
     4921 gaggcgatgt ccaagctggc caagacctcc tgaaacgcaa aaagcccccc tcccaaggac
     4981 actgagtcct aaagaggggg gtttcttgtc agtacgcgaa gaaccacgcc tggccgcgag
     5041 cgccagcacc gccgctctgt gcggagacct gggcaccagc cccgccgccg ccaggagcat
     5101 tgccgttccc gccagaaatc tagacggtga ccacaacgcg cccgctttga tcggggacgt
     5161 cgaaatattg gatcgtcggc accgtcacgg ccgtgggagg cggcacgatc cgcgacgtga
     5221 tgatcggccg catccccacg gtgctgcgca gtgagctcta cgccatcccg gcgttgatct
     5281 gtgcgttcgc acgcacaggc ccggtgtgag aagggtctct gacgagcggg agaactccct
     5341 atcag
//
