LOCUS       sequence-315544-        5969 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0248:Dendra
FEATURES             Location/Qualifiers
     source          1..5969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1913..1936
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2193..2993
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3118..3706
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3607..3626
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgtcacacga cgcgcaggcg gacgccgccc gcggccgagc
      121 gggctctgcg cgcggcaagt actggtggct gcgctgggtg ctcatcgccg tcgcggtcgc
      181 ggtgctcggt gtcgaactca cgctggtgtg ggatcagctc gccaaggcgt ggaagagcct
      241 ggtgtctgcg aacgtgtggt gggtgctggc cgcggtcgtc gcggcgatgg cctcgatcca
      301 cagcttcgcc cagatccagc ggacgctgtt gctgtcggcc ggggttcagg tgcggcagtg
      361 gcgttcggag gccgcgtact acgcgggcaa cgcgttgtcg acgacgatgc cgggcggtcc
      421 ggtgctctcg acgacgttca tctaccggca gcaacgcata tggggcgcat cgccgttggt
      481 ggcgtcgtgg cagttggtga tgtcgggtgt actgcagatc ctcggcctgg cgttgctcgg
      541 cctgggcggg gcgttcatgc tgggcgcgag caagaacccg ctgtcgctga tcttctcgct
      601 cggcgggttg ctcgcgttgg tgctgctcgg ccaggccgtc gcgtcgcggc ccgagttgat
      661 cgagggtatc ggcgtcaagg tgctgcactg ggtgaactcg gtgcgcggca agccatctga
      721 caccgggctg gccaagtggc gcgagatcct caaacagctg gaggcggtca agctgggccg
      781 ccgcgacctc acagtgtcgt tcagctggtc gatgttcaac tggatcgccg acgtcgcgtg
      841 cctggcgttc gcgtgctacg ccgcgggcgg tcatccgtcg ctggccgggg tcacggtggc
      901 atacgcggcc gcgcgtgcgg tcgggtcgat cccgctgatg cccggcggcc tgctggtcgt
      961 ggaggccgtg ctggtgcccg gtctggtgac ttcggggatg acgctggcct cggccatctc
     1021 cgcgatgctc atctaccgcc tgatcagctg gatcttcatc tccgcgatcg ggtgggtgat
     1081 cttcttcttc atgttccgca ccgagaccga tctcgacgtc gacagggtcg accagcacga
     1141 acccgcaccc gaacgccagg agcccaacaa gcttggtgcg gccgcgggca acacgcctgg
     1201 catcaatctg atcaaggagg acatgcgtgt caaggtgcat atggagggca acgtgaacgg
     1261 tcacgccttc gtgatcgagg gggagggtaa gggtaagcct tacgaaggga cccagaccgc
     1321 caacctcacc gtcaaggagg gcgcccctct cccattctcg tacgatatcc tcactaccgc
     1381 ggtgcattac gggaaccgcg tgttcaccaa gtaccccgag gatatccctg actacttcaa
     1441 gcagagcttc ccggagggat actcctggga gcggacgatg accttcgaag acaagggtat
     1501 ctgtacgatc cgcagcgaca tctcgctgga aggggactgt ttcttccaga acgtccgttt
     1561 caaggggacc aactttccgc cgaacggccc ggtcatgcag aagaagaccc tcaagtggga
     1621 gccgagcacg gagaagctgc acgttcggga cggcctcctg gtcggcaaca tcaacatggc
     1681 gctgctgctg gaaggcggcg gccactacct atgcgacttc aagaccacgt acaaagcgaa
     1741 gaaggtcgtc cagctgcccg acgcgcactt cgtggaccac cgcatcgaaa tcctggggaa
     1801 cgattcggac tacaacaagg tcaagttgta cgagcacgct gtggcacgct atagcccgct
     1861 cccgtcgcag gtctggttcg aaggcggcgg cggggagaac ctgtatttcc aggactacaa
     1921 ggacgacgac gacaagtgat ttaaataacc ccttggggcc tctaaacggg tcttgagggg
     1981 ttttttgtta actagcgtac gatcgaattc ctgcagctca cggtaactga tgccgtattt
     2041 gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc ctttgaatgg
     2101 gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcaccacc gactatttgc
     2161 aacagtgccg ttgatcgtgc tatgatcgac tgatgtcatc agcggtggag tgcaatgtcg
     2221 tgcaatacga atggcgaaaa gccgagctca tcggtcagct tctcaacctt ggggttaccc
     2281 ccggcggtgt gctgctggtc cacagctcct tccgtagcgt ccggcccctc gaagatgggc
     2341 cacttggact gatcgaggcc ctgcgtgcta cgctgggtcc gggagggacg ctcgtcatgc
     2401 cctcgtggtc aggtctggac gacgagccgt tcgatcctgc cacgtcgccc gttacaccgg
     2461 accttggagt tgtctctgac acattctggc gcctgccaaa tgtaaagcgc agcgcccatc
     2521 catttgcctt tgcggcagcg gggccacagg cagagcagat catctctgat ccattgcccc
     2581 tgccacctta ctcgcctgca agcccggtcg cccgtgtcca tgaactcgat gggcaggtac
     2641 ttctcctcgg cgtgggacac gatgccaaca cgacgctgca tcttgccgag ttgatggcaa
     2701 aggttcccta tggggtgccg agacactgca ccattcttca ggatggcaag ttggtacgcg
     2761 tcgattatct cgagaatgac cactgctgtg agcgctttgc cttggcggac aggtggctca
     2821 aggagaagag ccttcagaag gaaggtccag tcggtcatgc ctttgctcgg ttgatccgct
     2881 cccgcgacat tgtggcgaca gccctgggtc aactgggccg agatccgttg atcttcctgc
     2941 atccgccaga ggcgggatgc gaagaatgcg atgccgctcg ccagtcgatt ggctgagctc
     3001 atgagcggag aacgagatga cgttggaggg gcaaggtcgc gctgattgct ggggcaacac
     3061 gggggatcca ctagttccat gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3121 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3181 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3241 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     3301 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3361 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3421 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3481 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaggggag
     3541 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3601 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3661 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3721 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3781 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3841 cagccgaacg accgagcgca acgcgtgcgg ccgcggtacc cggggatcct ctagagtcga
     3901 ccaccaaggg caccatctct gcttgggcca ccccgttggc cgcagccagc tcgctgagag
     3961 ccgtgaacga cagggcgaac gccagcccgc cgacggcgag ggttccgacc gctgcaactc
     4021 ccggtgcaac cttgtcccgg tctattctct tcactgcacc agctccaatc tggtgtgaat
     4081 gcccctcgtc tgttcgcgca ggcggggggc tctattcgtt tgtcagcatc gaaagtagcc
     4141 agatcaggga tgcgttgcaa ccgcgtatgc ccaggtcaga agagtcgcac aagagttgca
     4201 gacccctgga aagaaaaatg gccagagggc gaaaacaccc tctgaccagc ggagcgggcg
     4261 acgggaatcg aacccgcgta gctagtttgg aagaatgggt gtctgccgac cacatatggg
     4321 ccggtcaaga taggttttta ccccctctcg gctgcatcct ctaagtggaa agaaattgca
     4381 ggtcgtagaa gcgcgttgaa gcctgagagt tgcacaggag ttgcaacccg gtagccttgt
     4441 tcacgacgag aggagaccta gttggcacgt cgcggatggg gatcgctgaa gactcagcgc
     4501 agcgggagga tccaagcctc atacgtcaac ccgcaggacg gtgtgaggta ctacgcgctg
     4561 cagacctacg acaacaagat ggacgccgaa gcctggctcg cgggcgagaa gcggctcatc
     4621 gagatggaga cctggacccc tccacaggac cgggcgaaga aggcagccgc cagcgccatc
     4681 acgctggagg agtacacccg gaagtggctc gtggagcgcg acctcgcaga cggcaccagg
     4741 gatctgtaca gcgggcacgc ggagcgccgc atctacccgg tgctaggtga agtggcggtc
     4801 acagagatga cgccagctct ggtgcgtgcg tggtgggccg ggatgggtag gaagcacccg
     4861 actgcccgcc ggcatgccta caacgtcctc cgggcggtga tgaacacagc ggtcgaggac
     4921 aagctgatcg cagagaaccc gtgccggatc gagcagaagg cagccgatga gcgcgacgta
     4981 gaggcgctga cgcctgagga gctggacatc gtcgccgctg agatcttcga gcactaccgg
     5041 atcgcggcat acatcctggc gtggacgagc ctccggttcg gagagctgat cgagcttcgc
     5101 cgcaaggaca tcgtggacga cggcatgacg atgaagctcc gggtgcgccg tggcgcttcc
     5161 cgcgtgggga acaagatcgt cgttggcaac gccaagaccg tccggtcgaa gcgtcctgtg
     5221 acggttccgc ctcacgtcgc ggagatgatc cgagcgcaca tgaaggaccg tacgaagatg
     5281 aacaagggcc ccgaggcatt cctggtgacc acgacgcagg gcaaccggct gtcgaagtcc
     5341 gcgttcacca agtcgctgaa gcgtggctac gccaagatcg gtcggccgga actccgcatc
     5401 cacgacctcc gcgctgtcgg cgctacgttc gccgctcagg caggtgcgac gaccaaggag
     5461 ctgatggccc gtctcggtca cacgactcct aggatggcga tgaagtacca gatggcgtct
     5521 gaggcccgcg acgaggctat cgctgaggcg atgtccaagc tggccaagac ctcctgaaac
     5581 gcaaaaagcc cccctcccaa ggacactgag tcctaaagag gggggtttct tgtcagtacg
     5641 cgaagaacca cgcctggccg cgagcgccag caccgccgct ctgtgcggag acctgggcac
     5701 cagccccgcc gccgccagga gcattgccgt tcccgccaga aatctagacg gtgaccacaa
     5761 cgcgcccgct ttgatcgggg acgtcgaaat attggatcgt cggcaccgtc acggccgtgg
     5821 gaggcggcac gatccgcgac gtgatgatcg gccgcatccc cacggtgctg cgcagtgagc
     5881 tctacgccat cccggcgttg atctgtgcgt tcgcacgcac aggcccggtg tgagaagggt
     5941 ctctgacgag cgggagaact ccctatcag
//
