LOCUS       sequence-315546-        6173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0759:Dendra
FEATURES             Location/Qualifiers
     source          1..6173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2117..2140
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2397..3197
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3322..3910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3811..3830
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgccggcaat cgtgctcatc ggggcccaat ggggcgacga
      121 gggcaaaggt aaagccaccg atctactcgg tggacgcgtg cagtgggtag ttcgctacca
      181 gggcggcaac aacgcgggac acaccgtcgt gttgcccacc ggggagaact tcgcgctgca
      241 cctcatcccc tcgggcatcc tcacacccgg ggtcaccaac gtcatcggta acggtgtggt
      301 ggtcgacccg ggcgtgctgt tgaccgagct gaagggcctc gaggatcgcg gggtcgacac
      361 gtcgaacctg ctgatctcgg ccgacgccca tctgctgatg ccgtaccacg tcgcgatcga
      421 caaggtcgtg gagcgctggg cgggcagcaa gaagatcggc accacgggcc gcggtatcgg
      481 cccgtgctac caggacaaga tcgcgcgcat cggcatccgg gtggccgacg tgctcgacga
      541 gcaggtgctg gccgaaaaga tcgaggccgc acttgaattc aagaaccagg tgctggtcaa
      601 gatctacaac cgcaaggcgc tggagcccgc cgaggtgctc gagaacctgc tggaacaggc
      661 cgagggcttc aagcatcgca tcgccgacgc gcggctgctg ctcaaccagg cgctggagaa
      721 cgacgaggcc gtgctgctcg agggctcgca gggcaccctg ctggatgtcg accacggcac
      781 gtatcccttt gtcacgtcgt cgaacccgac ggcaggcggc gccgcggtcg gctcgggcat
      841 cgggcccacc cgcatcacca cggtgctggg catcctcaag gcgtacacca cgcgcgtcgg
      901 ctccggtccg ttccccaccg aactgttcga cgagcacggc gcctacctgg ccaagaccgg
      961 tggcgaggtc ggtgtcacga ccggccgcgc ccgtcgatgc ggctggttcg acgcggtgat
     1021 cgcgcgctac gcgaccaggg tcaacggcat caccgactac ttcctgacca agctcgacgt
     1081 gctgtcgagc ctggagacag tgccggtgtg cgtcggctac acggtcgacg gcaagcgtgt
     1141 cgacgagatg ccgatgacgc agtcggacat cgcccgcgcc gagcccgtct acgaggaact
     1201 gccgggctgg tgggaggaca tctcgggcgc acgcgagttc gaggatctgc ccgccaaggc
     1261 ccgcgactac gtgctgcgcc tcgaagagct tgccggagcg tatgtttcgt gcatcggtgt
     1321 ggggccgggc cgggatcaga ccatcgtgcg ccgtgacgtg ctggctgccc gtaagcttgg
     1381 tgcggccgcg ggcaacacgc ctggcatcaa tctgatcaag gaggacatgc gtgtcaaggt
     1441 gcatatggag ggcaacgtga acggtcacgc cttcgtgatc gagggggagg gtaagggtaa
     1501 gccttacgaa gggacccaga ccgccaacct caccgtcaag gagggcgccc ctctcccatt
     1561 ctcgtacgat atcctcacta ccgcggtgca ttacgggaac cgcgtgttca ccaagtaccc
     1621 cgaggatatc cctgactact tcaagcagag cttcccggag ggatactcct gggagcggac
     1681 gatgaccttc gaagacaagg gtatctgtac gatccgcagc gacatctcgc tggaagggga
     1741 ctgtttcttc cagaacgtcc gtttcaaggg gaccaacttt ccgccgaacg gcccggtcat
     1801 gcagaagaag accctcaagt gggagccgag cacggagaag ctgcacgttc gggacggcct
     1861 cctggtcggc aacatcaaca tggcgctgct gctggaaggc ggcggccact acctatgcga
     1921 cttcaagacc acgtacaaag cgaagaaggt cgtccagctg cccgacgcgc acttcgtgga
     1981 ccaccgcatc gaaatcctgg ggaacgattc ggactacaac aaggtcaagt tgtacgagca
     2041 cgctgtggca cgctatagcc cgctcccgtc gcaggtctgg ttcgaaggcg gcggcgggga
     2101 gaacctgtat ttccaggact acaaggacga cgacgacaag tgatttaaat aaccccttgg
     2161 ggcctctaaa cgggtcttga ggggtttttt gttaactagc gtacgatcga attcctgcag
     2221 ctcacggtaa ctgatgccgt atttgcagta ccagcgtacg gcccacagaa tgatgtcacg
     2281 ctgaaaatgc cggcctttga atgggttcat gtgcagctcc atcagcaaaa ggggatgata
     2341 agtttatcac caccgactat ttgcaacagt gccgttgatc gtgctatgat cgactgatgt
     2401 catcagcggt ggagtgcaat gtcgtgcaat acgaatggcg aaaagccgag ctcatcggtc
     2461 agcttctcaa ccttggggtt acccccggcg gtgtgctgct ggtccacagc tccttccgta
     2521 gcgtccggcc cctcgaagat gggccacttg gactgatcga ggccctgcgt gctacgctgg
     2581 gtccgggagg gacgctcgtc atgccctcgt ggtcaggtct ggacgacgag ccgttcgatc
     2641 ctgccacgtc gcccgttaca ccggaccttg gagttgtctc tgacacattc tggcgcctgc
     2701 caaatgtaaa gcgcagcgcc catccatttg cctttgcggc agcggggcca caggcagagc
     2761 agatcatctc tgatccattg cccctgccac cttactcgcc tgcaagcccg gtcgcccgtg
     2821 tccatgaact cgatgggcag gtacttctcc tcggcgtggg acacgatgcc aacacgacgc
     2881 tgcatcttgc cgagttgatg gcaaaggttc cctatggggt gccgagacac tgcaccattc
     2941 ttcaggatgg caagttggta cgcgtcgatt atctcgagaa tgaccactgc tgtgagcgct
     3001 ttgccttggc ggacaggtgg ctcaaggaga agagccttca gaaggaaggt ccagtcggtc
     3061 atgcctttgc tcggttgatc cgctcccgcg acattgtggc gacagccctg ggtcaactgg
     3121 gccgagatcc gttgatcttc ctgcatccgc cagaggcggg atgcgaagaa tgcgatgccg
     3181 ctcgccagtc gattggctga gctcatgagc ggagaacgag atgacgttgg aggggcaagg
     3241 tcgcgctgat tgctggggca acacggggga tccactagtt ccatgagcgt cagaccccgt
     3301 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3361 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3421 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     3481 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3541 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3601 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3661 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3721 aagcgccacg cttcccgagg ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3781 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3841 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3901 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3961 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4021 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcaacgcgt gcggccgcgg
     4081 tacccgggga tcctctagag tcgaccacca agggcaccat ctctgcttgg gccaccccgt
     4141 tggccgcagc cagctcgctg agagccgtga acgacagggc gaacgccagc ccgccgacgg
     4201 cgagggttcc gaccgctgca actcccggtg caaccttgtc ccggtctatt ctcttcactg
     4261 caccagctcc aatctggtgt gaatgcccct cgtctgttcg cgcaggcggg gggctctatt
     4321 cgtttgtcag catcgaaagt agccagatca gggatgcgtt gcaaccgcgt atgcccaggt
     4381 cagaagagtc gcacaagagt tgcagacccc tggaaagaaa aatggccaga gggcgaaaac
     4441 accctctgac cagcggagcg ggcgacggga atcgaacccg cgtagctagt ttggaagaat
     4501 gggtgtctgc cgaccacata tgggccggtc aagataggtt tttaccccct ctcggctgca
     4561 tcctctaagt ggaaagaaat tgcaggtcgt agaagcgcgt tgaagcctga gagttgcaca
     4621 ggagttgcaa cccggtagcc ttgttcacga cgagaggaga cctagttggc acgtcgcgga
     4681 tggggatcgc tgaagactca gcgcagcggg aggatccaag cctcatacgt caacccgcag
     4741 gacggtgtga ggtactacgc gctgcagacc tacgacaaca agatggacgc cgaagcctgg
     4801 ctcgcgggcg agaagcggct catcgagatg gagacctgga cccctccaca ggaccgggcg
     4861 aagaaggcag ccgccagcgc catcacgctg gaggagtaca cccggaagtg gctcgtggag
     4921 cgcgacctcg cagacggcac cagggatctg tacagcgggc acgcggagcg ccgcatctac
     4981 ccggtgctag gtgaagtggc ggtcacagag atgacgccag ctctggtgcg tgcgtggtgg
     5041 gccgggatgg gtaggaagca cccgactgcc cgccggcatg cctacaacgt cctccgggcg
     5101 gtgatgaaca cagcggtcga ggacaagctg atcgcagaga acccgtgccg gatcgagcag
     5161 aaggcagccg atgagcgcga cgtagaggcg ctgacgcctg aggagctgga catcgtcgcc
     5221 gctgagatct tcgagcacta ccggatcgcg gcatacatcc tggcgtggac gagcctccgg
     5281 ttcggagagc tgatcgagct tcgccgcaag gacatcgtgg acgacggcat gacgatgaag
     5341 ctccgggtgc gccgtggcgc ttcccgcgtg gggaacaaga tcgtcgttgg caacgccaag
     5401 accgtccggt cgaagcgtcc tgtgacggtt ccgcctcacg tcgcggagat gatccgagcg
     5461 cacatgaagg accgtacgaa gatgaacaag ggccccgagg cattcctggt gaccacgacg
     5521 cagggcaacc ggctgtcgaa gtccgcgttc accaagtcgc tgaagcgtgg ctacgccaag
     5581 atcggtcggc cggaactccg catccacgac ctccgcgctg tcggcgctac gttcgccgct
     5641 caggcaggtg cgacgaccaa ggagctgatg gcccgtctcg gtcacacgac tcctaggatg
     5701 gcgatgaagt accagatggc gtctgaggcc cgcgacgagg ctatcgctga ggcgatgtcc
     5761 aagctggcca agacctcctg aaacgcaaaa agcccccctc ccaaggacac tgagtcctaa
     5821 agaggggggt ttcttgtcag tacgcgaaga accacgcctg gccgcgagcg ccagcaccgc
     5881 cgctctgtgc ggagacctgg gcaccagccc cgccgccgcc aggagcattg ccgttcccgc
     5941 cagaaatcta gacggtgacc acaacgcgcc cgctttgatc ggggacgtcg aaatattgga
     6001 tcgtcggcac cgtcacggcc gtgggaggcg gcacgatccg cgacgtgatg atcggccgca
     6061 tccccacggt gctgcgcagt gagctctacg ccatcccggc gttgatctgt gcgttcgcac
     6121 gcacaggccc ggtgtgagaa gggtctctga cgagcgggag aactccctat cag
//
