LOCUS       sequence-315551-        8990 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of GK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Orozco JM, Krawczyk PA, Scaria SM, Cangelosi AL, Chan SH, Kunchok T,
            Lewis CA, Sabatini DM
  TITLE     Dihydroxyacetone phosphate signals glucose availability to mTORC1.
  JOURNAL   Nat Metab. 2020 Sep;2(9):893-901. doi: 10.1038/s42255-020-0250-5. 
            Epub 2020 Jul 27.
  PUBMED    32719541
REFERENCE   2  (bases 1 to 8990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-020-0250-5"; journalName: "Nat Metab"; date: 
            "2020-09"; volume: "2"; issue: "9"; pages: "893-901"
COMMENT     Sequence Label: FLAG-GK in PLJM60
FEATURES             Location/Qualifiers
     source          1..8990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..37
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        19..37
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        65..291
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             292..472
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    519..644
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1141..1374
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1559..1603
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1910..2113
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2064..2084
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2110..2134
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2344..2362
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2344..2361
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2375..2394
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             2453..2476
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4148..4265
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4314..4824
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4743..4761
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4846..5445
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4846..4865)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5342..5362
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5573..5806
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5878..5999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5915..5934)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5969..5988
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6039..6174
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6194..6213)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6195..6213)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6223..6240)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6223..6239)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6232..6254)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6381..6836
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6468..6487)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6678..6699
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6862..6966
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6967..7035,7036..7827)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7185..7204)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7998..8586
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8487..8506
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8740..8757
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8874..8895
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8910..8915,8916..8933,8934..8940)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8948..8964
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8953..8975
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8972..8988
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8972..8988
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
       61 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
      121 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      181 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      241 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      301 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      361 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      421 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      481 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      541 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      601 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      661 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      721 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      781 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      841 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      901 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      961 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     1021 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1081 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1141 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1201 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1261 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1321 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1381 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1441 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1501 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1561 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1621 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1681 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1741 atcgtttcag acccacctcc caaccccgag gggaccccat atgccaagta cgccccctat
     1801 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     1861 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     1921 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     1981 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2041 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     2101 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     2161 tgacctccat agaagacacc gggaccgatc cagcctccgc ggccgggaac ggtgcattgg
     2221 aacgcggatt ccccgtgcca agagtgacgt aagtaccgcc tatagagtct ataggcccac
     2281 ccccttggct tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat
     2341 acgatttagg tgacactata gaataacatc cactttgcct ttctctccac aggtgtccac
     2401 tcccaggtcc aactgcacct cggttctatc gattgaattc accatggaaa tggactacaa
     2461 ggatgacgat gacaagggtg gtggtggtgg tgcgtcgaca atggcagcct caaagaaggc
     2521 agttttgggg ccattggtgg gggcggtgga ccagggcacc agttcgacgc gctttttggt
     2581 tttcaattca aaaacagctg aactacttag tcatcatcaa gtagaaataa aacaagagtt
     2641 cccaagagaa ggatgggtgg aacaggaccc taaggaaatt ctacattctg tctatgagtg
     2701 tatagagaaa acatgtgaga aacttggaca gctcaaaatt gatatttcca acataaaagc
     2761 tattggtgtc agcaaccaga gggaaaccac tgtagtctgg gacaagataa ctggagagcc
     2821 tctctacaat gctgtggtgt ggcttgatct aagaacccag tctaccgttg agagtcttag
     2881 taaaagaatt acaggaaata ataactttgt caagtccaag acaggccttc cacttagcac
     2941 ttacttcagt gcagtgaaac ttcgttggct ccttgacaat gtgagaaaag ttcaaaaggc
     3001 agttgaagaa aaacgagctc tttttgggac tattgattca tggcttattt ggagtttgac
     3061 aggaggagtc aatggaggtg tccactgtac agatgtaaca aatgcaagta ggactatgct
     3121 tttcaacatt cattctttgg aatgggataa acaactctgc gaattttttg gaattccaat
     3181 ggaaattctt ccaaatgtcc ggagttcttc tgagatctat ggcctaatga aaatctctca
     3241 tagcgtgaaa gctggggcct tggaaggtgt gccaatatct gggtgtttag gggaccagtc
     3301 tgctgcattg gtgggacaaa tgtgcttcca gattggacaa gccaaaaata cgtatggaac
     3361 aggatgtttc ttactatgta atacaggcca taagtgtgta ttttctgatc atggccttct
     3421 caccacagtg gcttacaaac ttggcagaga caaaccagta tattatgctt tggaaggttc
     3481 tgtagctata gctggtgctg ttattcgctg gctaagagac aatcttggaa ttataaagac
     3541 ctcagaagaa attgaaaaac ttgctaaaga agtaggtact tcttatggct gctacttcgt
     3601 cccagcattt tcggggttat atgcacctta ttgggagccc agcgcaagag ggataatctg
     3661 tggactcact cagttcacca ataaatgcca tattgctttt gctgcattag aagctgtttg
     3721 tttccaaact cgagagattt tggatgccat gaatcgagac tgtggaattc cactcagtca
     3781 tttgcaggta gatggaggaa tgaccagcaa caaaattctt atgcagctac aagcagacat
     3841 tctgtatata ccagtagtga agccctcaat gcccgaaacc actgcactgg gtgcggctat
     3901 ggcggcaggg gctgcagaag gagtcggcgt atggagtctc gaacccgagg atttgtctgc
     3961 cgtcacgatg gagcggtttg aacctcagat taatgcggag gaaagtgaaa ttcgttattc
     4021 tacatggaag aaagctgtga tgaagtcaat gggttgggtt acaactcaat ctccagaaag
     4081 tggtattcca taagcggccg cagtgagaat tctcgacctc gagacaaatg gcagtattca
     4141 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     4201 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     4261 attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg
     4321 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     4381 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     4441 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     4501 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     4561 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     4621 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     4681 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     4741 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     4801 cgaatcaccg acctctctcc ccagggggat ccaccggagc ttaccatgac cgagtacaag
     4861 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     4921 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     4981 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5041 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     5101 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     5161 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     5221 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     5281 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     5341 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     5401 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
     5461 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cggtaccttt aagaccaatg
     5521 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     5581 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     5641 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     5701 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     5761 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     5821 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     5881 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5941 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6001 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6061 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6121 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     6181 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     6241 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     6301 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     6361 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     6421 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     6481 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     6541 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     6601 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     6661 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     6721 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     6781 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     6841 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     6901 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     6961 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7021 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7081 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7141 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7201 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7261 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     7321 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     7381 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     7441 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     7501 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     7561 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     7621 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     7681 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     7741 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     7801 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     7861 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     7921 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7981 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8041 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8101 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8161 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8221 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8281 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8341 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8401 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8461 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     8521 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8581 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     8641 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     8701 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     8761 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     8821 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     8881 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     8941 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
//
