LOCUS       sequence-315552-        1216 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA destination plasmid containing restriction sites for golden 
            gate cloning. Suitable for amber suppression (4xMmaPylT cassette); 
            for transient expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4x7SKPylT_EF1_Ub-BsmBI
FEATURES             Location/Qualifiers
     source          1..1216
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..305
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(383..403)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    445..566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(482..501)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     536..555
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctttgggttt ccgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
       61 tctagagcta gcgaattatg cagatcttcg tgaagactct gactggtaag accatcaccc
      121 tcgaggttga gcccagtgac accatcgaga atgtcaaggc aaagatccaa gataaggaag
      181 gcatccctcc tgaccagcag aggctgatct ttgctggaaa acagctggaa gatgggcgca
      241 ccctgtctga ctacaacatc cagaaagagt ccaccctgca cctggtgctc cgcttaagag
      301 ggggtagaga cgatcgtctc accggcgaca atcaacctct ggattacaaa atttgtgaaa
      361 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
      421 tgcctttgta tcatgcgtta actaaacttg tttattgcag cttataatgg ttacaaataa
      481 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      541 ttgtccaaac tcatcaatgt atcttatcat gtctggaatt gactcaaatg atgtcaatta
      601 gtctatcaga agctcatctg gtctcccttc cgggggacaa gacatccctg tttaatattt
      661 aaacagcagt gttcccaaac tgggttctta tatcccttgc tctggtcaac caggttgcag
      721 ggtttcctgt cctcacagga acgaagtccc taaagaaaca gtggcagcca ggtttagccc
      781 cggaattgac tggattcctt ttttagggcc cattggtatg gctttttccc cgtatccccc
      841 caggtgtctg caggctcaaa gagcagcgag aagcggtcag aggaaagcga tcccgtgcca
      901 cctttcccgt gcccgggctg gccccgcacg ctgccggctc ggggatgcgg ggggagcgcc
      961 ggaccgaacc gaaccccggg cggctcgctg ctgcccctag cgggggaggg acgtattaca
     1021 tccctggggg ctttgggggg ggctgccccg gattcttaac aggaaaattt ttaaaaggtt
     1081 tcccggagtg gacctgaaag aacaaataat ttccctgggg taaacttaaa ggccctaaaa
     1141 aaaccggggc ttttgacccc gggccttaag gttcaaacgg ggccttccgc tggaaggccc
     1201 ctcgggggcc tcgggg
//
