LOCUS       sequence-315553-        1252 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA destination plasmid with HA terminal tag; Suitable for amber 
            suppression (4xMmaPylT cassette); for transient expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA
FEATURES             Location/Qualifiers
     source          1..1252
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             91..318
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             335..361
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(426..446)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    488..609
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(525..544)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     579..598
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ggtaaattgg gttttaaaag actgctcgtt ctgttctgcg ccgttacaga tccaagctgt
       61 gaccggcgcc tactctagag ctagcgaatt atgcagatct tcgtgaagac tctgactggt
      121 aagaccatca ccctcgaggt tgagcccagt gacaccatcg agaatgtcaa ggcaaagatc
      181 caagataagg aaggcatccc tcctgaccag cagaggctga tctttgctgg aaaacagctg
      241 gaagatgggc gcaccctgtc tgactacaac atccagaaag agtccaccct gcacctggtg
      301 ctccgcttaa gagggggtag agacgatcgt ctcataccca tacgatgttc cagattacgc
      361 ttaaccggcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
      421 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgcg
      481 ttaactaaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
      541 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
      601 tgtatcttat catgtctgga attgactcaa atgatgtcaa ttagtctatc agaagctcat
      661 ctggtctccc ttccggggga caagacatcc ctgtttaata tttaaacagc agtgttccca
      721 aactgggttc ttatatccct tgctctggtc aaccaggttg cagggtttcc tgtcctcaca
      781 ggaacgaagt ccctaaagaa acagtggcag ccaggtttag ccccggaatt gactggattc
      841 cttttttagg gcccattggt atggcttttt cccgataatc cccccaggtg tctgcaggct
      901 caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg
      961 gctgtccccg cacgctgccg gctcggggat gcggggggaa cgcccggacc ggaacggaac
     1021 cccgggcggg ctcgcggcgg ccccctaacg ggggagggac gtaattacat ccctgggggc
     1081 tttggggggg gggctggccc tggaaatcta aaaacagaaa aaatttatta aaaaagtttt
     1141 ccggggaggt ggaacctgga aaaaacaata aattttccgg aggggttaat cttaaaggcc
     1201 cggaaaagaa aaccgcggcc cttttggccc cccgggcctt tggtcaaaac cg
//
