LOCUS       sequence-315555-        1206 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA destination plasmid containing restriction sites for golden 
            gate cloning. Suitable for amber suppression (4xMmaPylT cassette); 
            for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD
FEATURES             Location/Qualifiers
     source          1..1206
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..324
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     misc_feature    join(375..949,950..952,953..961)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(542..559)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     769..788
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gacattgaat cgtttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctactc
       61 tagagctagc gaattcgaat ttaaatcgga tccaccatgc agatcttcgt gaagactctg
      121 actggtaaga ccatcaccct cgaggttgag cccagtgaca ccatcgagaa tgtcaaggca
      181 aagatccaag ataaggaagg catccctcct gaccagcaga ggctgatctt tgctggaaaa
      241 cagctggaag atgggcgcac cctgtctgac tacaacatcc agaaagagtc caccctgcac
      301 ctggtgctcc gcttaagagg gggtagagac gatcgtctca ccggcgacaa tcaacctctg
      361 gtcgagcggc cgcgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
      421 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
      481 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
      541 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
      601 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
      661 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
      721 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
      781 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
      841 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
      901 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac
      961 catggcgtcc ggaatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     1021 aacggctaca atcaacagca tccccatctc tgaagaatac agcgtcgcca gcgcagctct
     1081 ttctagggac ggccgcatct tcacgggggg caagggatat cattttactg gggggacctt
     1141 gtgcaaaaat ctgggtgctg gggactggtt gtgcttgggg aactgggaac ccgaattgtt
     1201 accccc
//
