LOCUS       sequence-315556-        1180 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA destination plasmid with HA terminal tag; Suitable for amber 
            suppression (4xMmaPylT cassette); for transient or stable 
            piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD
FEATURES             Location/Qualifiers
     source          1..1180
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             96..323
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             340..366
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(404..979,980..982,983..991)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(571..588)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     798..817
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gacattgatt cgttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctactct
       61 agagctagcg aattcgaatt taaatcggat ccaccatgca gatcttcgtg aagactctga
      121 ctggtaagac catcaccctc gaggttgagc ccagtgacac catcgagaat gtcaaggcaa
      181 agatccaaga taaggaaggc atccctcctg accagcagag gctgatcttt gctggaaaac
      241 agctggaaga tgggcgcacc ctgtctgact acaacatcca gaaagagtcc accctgcacc
      301 tggtgctccg cttaagaggg ggtagagacg atcgtctcat acccatacga tgttccagat
      361 tacgcttaac cggcgacaat caacctctgg tcgagcggcc gcgcccctct ccctcccccc
      421 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
      481 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
      541 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
      601 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
      661 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
      721 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
      781 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
      841 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
      901 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cgggggacgt ggttttcctt
      961 tgaaaaacac gatgataata tggccacaac catgggcgtc cggaatggcc aagcctttgt
     1021 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaaaagc atccccatct
     1081 ctgaaaacta cagcgtcccc agcgcagctc tttctaacga cggccgcatc ttcactgggg
     1141 ccaaggtatt cattttacgg ggggaccttg ggcaaaagtg
//
