LOCUS       sequence-315557-        1496 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA plasmid for amber suppression of N-terminally labelled GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4x7SKPylT_EF1_Ub-*GFP
FEATURES             Location/Qualifiers
     source          1..1496
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..303
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             1024..1041
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gaacttggat cgtttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctactc
       61 tagagctagc gaattatgca gatcttcgtg aagactctga ctggtaagac catcaccctc
      121 gaggttgagc ccagtgacac catcgagaat gtcaaggcaa agatccaaga taaggaaggc
      181 atccctcctg accagcagag gctgatcttt gctggaaaac agctggaaga tgggcgcacc
      241 ctgtctgact acaacatcca gaaagagtcc accctgcacc tggtgctccg cttaagaggg
      301 ggttagtcca agggcgagga actgttcacc ggcgtggtgc ccatcctggt ggaactggat
      361 ggcgacgtga acggccacaa gttctctgtg cggggagagg gcgaaggcga cgccacaaat
      421 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ttggcctacc
      481 ctcgtgacca cactgaccta cggcgtgcag tgcttcagca gataccccga ccatatgaag
      541 cggcacgact tcttcaagag cgccatgccc gagggctacg tgcaggaacg gaccatcagc
      601 ttcaaggacg acggcaccta caagaccaga gccgaagtga agttcgaggg cgacaccctc
      661 gtgaaccgga tcgagctgaa gggcatcgat ttcaaagagg acggcaacat cctgggccac
      721 aagctggagt acaacttcaa cagccacctg gtgtacatca ccgccgacaa gcagaagaac
      781 ggcatcaagg ccaacttcaa gatccggcac aacgtggaag atggcagcgt gcagctggcc
      841 gaccactacc agcagaacac ccccatcgga gatggccccg tgctgctgcc cgacaaccac
      901 tacctgagca cccagagcgt gctgagcaag gaccccaacg agaagcggga ccacatggtg
      961 ctgctggaat tcgtgaccgc cgctggcatc acccacggca tggacgagct gtacaagggc
     1021 agccaccatc accatcacca ttaaccggcg acaatcaacc tctggattac aaatttgtga
     1081 agattgactg ggattcttaa caagttgctc ctttacgcta gtggataccc ggcttaaggc
     1141 cttggatcag gcgtaacaaa cttgttattg cgccttaaag ggttcaaaaa agcaaaacct
     1201 cccaatttcc aaaaacattt ttcccggctt caattgggtt gcaccacaca ggatttatac
     1261 gcggagaaca caaggaaaaa caacaacaac aggcgcctcc gggagaagtg ttattaaacg
     1321 tctgcggctt tcctgttgct tggtggtggt cgcatgaaca acaaaggaat agttagtttg
     1381 gatgtttctt tcattgcatc ggatcccttt cgtcgcaaag cgcagcacgc aacgcacggg
     1441 caaggcggta ggaagggatt acctgagccc gcccctttaa aagggggaaa cttttt
//
