LOCUS       sequence-315558-        1426 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA plasmid for amber suppression of N-terminally labelled 
            NoBody.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4x7SKPylT_EF1_Ub-*NoBody
FEATURES             Location/Qualifiers
     source          1..1426
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..305
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(574..594)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    636..757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(673..692)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     727..746
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cgccctctgt tcggtttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
       61 tctagagcta gcgaattatg cagatcttcg tgaagactct gactggtaag accatcaccc
      121 tcgaggttga gcccagtgac accatcgaga atgtcaaggc aaagatccaa gataaggaag
      181 gcatccctcc tgaccagcag aggctgatct ttgctggaaa acagctggaa gatgggcgca
      241 ccctgtctga ctacaacatc cagaaagagt ccaccctgca cctggtgctc cgcttaagag
      301 ggggttaggg agaccaacct tgtgcctccg ggagatccac tctcccacct ggaaacgcac
      361 gggaagccaa gcctccaaaa aagcgctgcc tcctcgctcc gcgttgggat tatccggaag
      421 gaactcccaa cggaggtagt accactctac cctccgcacc tcctcctgca tcagccggcc
      481 tgaagtcgca ccctcctcct ccggagaagt gaccggcgac aatcaacctc tggattacaa
      541 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
      601 cgctgcttta atgcctttgt atcatgcgtt aactaaactt gtttattgca gcttataatg
      661 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
      721 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggaat tgactcaaat
      781 gatgtcaatt agtctatcag aagctcatct ggtctccctt ccgggggaca agacatccct
      841 gtttaatatt taaacagcag tgttcccaaa ctgggttctt atatcccttg ctctggtcaa
      901 ccaggttgca gggtttcctg tcctcacagg aacgaagtcc ctaaagaaac agtggcagcc
      961 aggtttagcc ccggaattga ctggattcct tttttagggc ccattggtat ggctttttcc
     1021 ccgtaatccc cccaggtgtc tgcaggctca aagagcagcg agaagcgttc agaggaaagc
     1081 gatcccgtgc caccttcccc gtgcccgggc tgtccccgca cgctgccggc tcggggatgc
     1141 ggggggagcg cccgaaccgg aacggaaccc cgggcggctc gctgctgccc cctagcgggg
     1201 gagggacgta attacatccc tggggggctt tggggggggg gctgtccctg aattctataa
     1261 caagaaaata attatataat aagttatccc gtaggtagaa cctggaaata acaatttaat
     1321 tttcgtatga gttaaacctt aaaagtcccg taaaagataa tcaggggtct ttttgacccc
     1381 ccggggcctt aaaagttaaa aaaatagtga accttacccc tttgga
//
