LOCUS       sequence-315559-        1206 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA plasmid for amber suppression of N-terminally labelled 
            SMIM38.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4x7SKPylT_EF1_Ub-*SMIM38
FEATURES             Location/Qualifiers
     source          1..1206
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             77..304
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(522..542)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    584..705
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(621..640)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     675..694
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ggaacaagat cgatttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctact
       61 ctagagctag cgaattatgc agatcttcgt gaagactctg actggtaaga ccatcaccct
      121 cgaggttgag cccagtgaca ccatcgagaa tgtcaaggca aagatccaag ataaggaagg
      181 catccctcct gaccagcaga ggctgatctt tgctggaaaa cagctggaag atgggcgcac
      241 cctgtctgac tacaacatcc agaaagagtc caccctgcac ctggtgctcc gcttaagagg
      301 gggttagacc tcctggccag gtggcagctt tggccctgac ccgctcctgg ccctgctggt
      361 ggtgatcctg ctagcacgcc tcatcctgtg gtcctgcctc gggacctaca tcgactacag
      421 actggcccag cggcggcccc agaaacccaa gcaggactaa ccggcgacaa tcaacctctg
      481 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
      541 tgtggatacg ctgctttaat gcctttgtat catgcgttaa ctaaacttgt ttattgcagc
      601 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
      661 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggaattg
      721 actcaaatga tgtcaattag tctatcagaa gctcatctgg tctcccttcc gggggacaag
      781 acatccctgt ttaatattta aacagcagtg ttcccaaact gggttcttat atcccttgct
      841 ctggtcaacc aggttgcagg gtttcctgtc ctcacaggaa cgaagtccct aaagaaacag
      901 tggcagccag gtttagcccc ggaattgact ggattccttt tttagggccc attggtatgg
      961 ctttttcgcg cgttatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca
     1021 gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtcccccacg ctgccggctc
     1081 ggggatgcgg ggggaacgcc ggaacggaac ggaaccccgg gcggttgctg ctgcccccaa
     1141 cgggggaggg actaattata ccccgggggg ggtttttggg gggggggtcc cgggaattaa
     1201 aaaaaa
//
