LOCUS       sequence-315560-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA plasmid for amber suppression of N-terminally labelled 
            PIGBOS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS_4x7SKPylT_EF1_Ub-*PIGBOS
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             74..301
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(528..548)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    590..711
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(627..646)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     681..700
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctttggctcg tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctactcta
       61 gagctagcga attatgcaga tcttcgtgaa gactctgact ggtaagacca tcaccctcga
      121 ggttgagccc agtgacacca tcgagaatgt caaggcaaag atccaagata aggaaggcat
      181 ccctcctgac cagcagaggc tgatctttgc tggaaaacag ctggaagatg ggcgcaccct
      241 gtctgactac aacatccaga aagagtccac cctgcacctg gtgctccgct taagaggggg
      301 ttagtttagg agattgactt ttgcacaact gctttttgcc actgtccttg gaattgctgg
      361 aggagtatat atttttcaac cagtatttga acagtatgcc aaagatcaga aggaattaaa
      421 agaaaagatg cagttggtac aagaatcaga agagaagaaa agttaaccgg cgacaatcaa
      481 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
      541 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg cgttaactaa acttgtttat
      601 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
      661 tttttcactg cattctattt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
      721 gaattgactc caatgatgtc aattagccaa tcataagctc ctctggtcct cccctccagg
      781 gggacagatc accccagctt catcagttaa gcagggctgc tgccgaaatg cttctatatc
      841 cgatgatact gtcagaccaa gttggaagtg tctcagactc ccggaaccaa gtccgctcaa
      901 gacaactagt cggcagtact caatccgcag gatttacttg acatctgtat acgagaccat
      961 gcataggact gctatcgcta ggctacgcct aatgactaa
//
