LOCUS       sequence-315561-        1154 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA destination plasmid containing restriction sites for golden 
            gate cloning. Suitable for amber suppression (4xMmaPylT cassette); 
            for transient expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD
FEATURES             Location/Qualifiers
     source          1..1154
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             605..928
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLGDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQGAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     ELE"
     primer_bind     complement(987..1007)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 agaacatgaa acgttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctactc
       61 tagagctagc gaattcgcca ccagagacgg ttccgtctca tgcctcagtt ttggcaccga
      121 aattttaacc gttgagtacg gcccattgcc cattggcaaa attgtgagtg aagaaattaa
      181 ttgttctgtg tacagtgttg atccagaagg gagagtttac acccaggcga tcgcccaatg
      241 gcatgaccgg ggagagcagg aagtattgga atatgaattg gaagatggtt cagtaatccg
      301 agctacctct gaccaccgct ttttaaccac cgattatcaa ctgttggcga tcgaagaaat
      361 ttttgctagg caactggact tgttgacttt agaaaatatt aagcaaactg aagaagctct
      421 tgacaaccat cgtcttccct ttccattact tgacgctggg acaattaaaa tggttaaagt
      481 tatcggtcgt cgttccctcg gagtgcaaag aatatttgat attggtcttc cccaagacca
      541 taattttctg ctagccaatg gggcgatcgc cgccgcctgc ttcaacgaat tcgaattcct
      601 cgagggagtg caggtggaaa ccatctcccc aggtgacggg cgcaccttcc ccaagcgcgg
      661 ccagacctgc gtggtgcact acaccgggat gcttggagat ggaaagaaag ttgactcctc
      721 ccgggacaga aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg
      781 ggaagaaggg gttgcccaga tgagtgtggg tcagggagcc aaactgacta tatctccaga
      841 ttatgcctat ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt
      901 cgatgtggag cttctagaac tggaatgatc gacaatcaac ctctggatta caaaatttgt
      961 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     1021 ttaaggcctt tgtatcatgc gttaactaaa cttggttatt ggcaccttaa aagggttaca
     1081 aataaagcaa tagcctccca aattttccaa ataaagcatt tttttcccgg gctttccaat
     1141 tgggggttgg ccca
//
