LOCUS       sequence-315562-        1188 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control STELLA destination plasmid for C-terminal labelled proteins.
            Contains restriction sites for golden gate cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD
FEATURES             Location/Qualifiers
     source          1..1188
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             604..927
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLGDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQGAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     ELE"
     primer_bind     complement(986..1006)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 atgactgatc gattctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctactct
       61 agagctagcg aattcgccac cagagacggt tccgtctcag ccctcagttt tggcaccgaa
      121 attttaaccg ttgagtacgg cccattgccc attggcaaaa ttgtgagtga agaaattaat
      181 tgttctgtgt acagtgttga tccagaaggg agagtttaca cccaggcgat cgcccaatgg
      241 catgaccggg gagagcagga agtattggaa tatgaattgg aagatggttc agtaatccga
      301 gctacctctg accaccgctt tttaaccacc gattatcaac tgttggcgat cgaagaaatt
      361 tttgctaggc aactggactt gttgacttta gaaaatatta agcaaactga agaagctctt
      421 gacaaccatc gtcttccctt tccattactt gacgctggga caattaaaat ggttaaagtt
      481 atcggtcgtc gttccctcgg agtgcaaaga atatttgata ttggtcttcc ccaagaccat
      541 aattttctgc tagccaatgg ggcgatcgcc gccgcctgct tcaacgaatt cgaattcctc
      601 gagggagtgc aggtggaaac catctcccca ggtgacgggc gcaccttccc caagcgcggc
      661 cagacctgcg tggtgcacta caccgggatg cttggagatg gaaagaaagt tgactcctcc
      721 cgggacagaa acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg
      781 gaagaagggg ttgcccagat gagtgtgggt cagggagcca aactgactat atctccagat
      841 tatgcctatg gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc
      901 gatgtggagc ttctagaact ggaatgatcg acaatcaacc tctggattac aaaatttgtg
      961 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     1021 taaggccttt ggatcaggcg ttaactaaac ttggttattg cgacctttaa aggggttcca
     1081 aaaaagcaat agcctcccaa atttcccaaa taaagccttt ttttcccggg ctcccaattg
     1141 ggggttggcc aaacccccca ggggttttta aacggggggg ggaatgcc
//
