LOCUS       sequence-315563-        1507 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA plasmid for amber suppression of C-terminally labelled GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4xh7SKPylT_EF1_GFP*_Intein_DD
FEATURES             Location/Qualifiers
     source          1..1507
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             801..818
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aactggatcg attctgttct gcgccgttac agatccaagc tgtgaccggc gcctactcta
       61 gagctagcga attcgccacc atgtccaagg gcgaggaact gttcaccggc gtggtgccca
      121 tcctggtgga actggatggc gacgtgaacg gccacaagtt ctctgtgcgg ggagagggcg
      181 aaggcgacgc cacaaatggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      241 ccgtgccttg gcctaccctc gtgaccacac tgacctacgg cgtgcagtgc ttcagcagat
      301 accccgacca tatgaagcgg cacgacttct tcaagagcgc catgcccgag ggctacgtgc
      361 aggaacggac catcagcttc aaggacgacg gcacctacaa gaccagagcc gaagtgaagt
      421 tcgagggcga caccctcgtg aaccggatcg agctgaaggg catcgatttc aaagaggacg
      481 gcaacatcct gggccacaag ctggagtaca acttcaacag ccacctggtg tacatcaccg
      541 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccggcacaac gtggaagatg
      601 gcagcgtgca gctggccgac cactaccagc agaacacccc catcggagat ggccccgtgc
      661 tgctgcccga caaccactac ctgagcaccc agagcgtgct gagcaaggac cccaacgaga
      721 agcgggacca catggtgctg ctggaattcg tgaccgccgc tggcatcacc cacggcatgg
      781 acgagctgta caagggcagc caccatcacc atcaccatta gtgcctcagt tttggcaccg
      841 aaattttaac cgttgagtac ggcccattgc ccattggcaa aattgtgagt gaagaaatta
      901 attgttctgt gtacagtgtt gatccagaag ggagagttta cacccaggcg atcgcccaat
      961 ggcatgaccg gggagagcag gaagtattgg atatgaattg gaagatggtt cagtaatccg
     1021 agctacctct gaccacgctt tttaacaccg attatcaact gttggcgatc gagaaatttt
     1081 tgctaggcaa ctggacttgt tgactttaaa atattaacca actgagaaac tcttgacacc
     1141 acgccttcct ttcataattg acctgggaca taaaagggta aaatttcggc ccttccctcg
     1201 aaggaaaaaa atttgaattg gtttccccaa caaatttttt gttccccaag ggggagcccc
     1261 cggtcaaaaa atattttctg gggggggggg aacacctccc gcggggcccc cccccccccc
     1321 cgctctcctc ctgcttaatg aataactacc tcacccccaa tcattcaggt ggaggcggga
     1381 gggaaagaat gtaagggaac ccaattcaag ttccactaca cttttcaaaa tttaagttag
     1441 gtcttgtaat gttcgatcgt caggcgcatt cgtgccccag gctgccctca gctcctacct
     1501 gaagcgt
//
