LOCUS       sequence-315564-        1516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA plasmid for amber suppression of C-terminally labelled 
            NoBody.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4xh7SKPylT_EF1_NoBody*_Intein_DD
FEATURES             Location/Qualifiers
     source          1..1516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      80..89
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             797..1120
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLGDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQGAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     ELE"
     primer_bind     complement(1179..1199)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cgtccctctg ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta
       61 ctctagagct agcgaattcg ccaccatggg agaccaacct tgtgcctccg ggagatccac
      121 tctcccacct ggaaacgcac gggaagccaa gcctccaaaa aagcgctgcc tcctcgctcc
      181 gcgttgggat tatccggaag gaactcccaa cggaggtagt accactctac cctccgcacc
      241 tcctcctgca tcagccggcc tgaagtcgca ccctcctcct ccggagaagt agtgcctcag
      301 ttttggcacc gaaattttaa ccgttgagta cggcccattg cccattggca aaattgtgag
      361 tgaagaaatt aattgttctg tgtacagtgt tgatccagaa gggagagttt acacccaggc
      421 gatcgcccaa tggcatgacc ggggagagca ggaagtattg gaatatgaat tggaagatgg
      481 ttcagtaatc cgagctacct ctgaccaccg ctttttaacc accgattatc aactgttggc
      541 gatcgaagaa atttttgcta ggcaactgga cttgttgact ttagaaaata ttaagcaaac
      601 tgaagaagct cttgacaacc atcgtcttcc ctttccatta cttgacgctg ggacaattaa
      661 aatggttaaa gttatcggtc gtcgttccct cggagtgcaa agaatatttg atattggtct
      721 tccccaagac cataattttc tgctagccaa tggggcgatc gccgccgcct gcttcaacga
      781 attcgaattc ctcgagggag tgcaggtgga aaccatctcc ccaggtgacg ggcgcacctt
      841 ccccaagcgc ggccagacct gcgtggtgca ctacaccggg atgcttggag atggaaagaa
      901 agttgactcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt
      961 gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagggag ccaaactgac
     1021 tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc
     1081 cactctcgtc ttcgatgtgg agcttctaga actggaatga tcgacaatca acctctggat
     1141 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     1201 ggatacgctg ctttaatgcc tttgaatcat gcgttaacta aacttgttta ttgcagctta
     1261 aaagggttac aaataaagca atagcatccc aaattttcca aaataaagca ttttttttcc
     1321 cggcattcca aattgggggt ttggcccaaa cccctcaagg gtttttaacc gggttgggaa
     1381 attgcccccc aagggaggtg aaatttatac ttttaaaaaa aaacccttcc gggggctccc
     1441 tctctccgcg ggaaaaaaaa aatccctgtt tttaaatttt ttaaaaacac agggttgttc
     1501 cccaaagggg gggttt
//
