LOCUS       sequence-315565-        1363 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA plasmid for amber suppression of C-terminally labelled 
            SMIM38.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD
FEATURES             Location/Qualifiers
     source          1..1363
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             751..1074
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLGDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQGAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     ELE"
     primer_bind     complement(1133..1153)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    1195..1276
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cctattctac cctttggttg tttctgttct gcgccgttac agatccaagc tgtgaccggc
       61 gcctactcta gagctagcga attcgccacc atgacctcct ggccaggtgg cagctttggc
      121 cctgacccgc tcctggccct gctggtggtg atcctgctag cacgcctcat cctgtggtcc
      181 tgcctcggga cctacatcga ctacagactg gcccagcggc ggccccagaa acccaagcag
      241 gactagtgcc tcagttttgg caccgaaatt ttaaccgttg agtacggccc attgcccatt
      301 ggcaaaattg tgagtgaaga aattaattgt tctgtgtaca gtgttgatcc agaagggaga
      361 gtttacaccc aggcgatcgc ccaatggcat gaccggggag agcaggaagt attggaatat
      421 gaattggaag atggttcagt aatccgagct acctctgacc accgcttttt aaccaccgat
      481 tatcaactgt tggcgatcga agaaattttt gctaggcaac tggacttgtt gactttagaa
      541 aatattaagc aaactgaaga agctcttgac aaccatcgtc ttccctttcc attacttgac
      601 gctgggacaa ttaaaatggt taaagttatc ggtcgtcgtt ccctcggagt gcaaagaata
      661 tttgatattg gtcttcccca agaccataat tttctgctag ccaatggggc gatcgccgcc
      721 gcctgcttca acgaattcga attcctcgag ggagtgcagg tggaaaccat ctccccaggt
      781 gacgggcgca ccttccccaa gcgcggccag acctgcgtgg tgcactacac cgggatgctt
      841 ggagatggaa agaaagttga ctcctcccgg gacagaaaca agccctttaa gtttatgcta
      901 ggcaagcagg aggtgatccg aggctgggaa gaaggggttg cccagatgag tgtgggtcag
      961 ggagccaaac tgactatatc tccagattat gcctatggtg ccactgggca cccaggcatc
     1021 atcccaccac atgccactct cgtcttcgat gtggagcttc tagaactgga atgatcgaca
     1081 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     1141 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgcgtta actaaacttg
     1201 tttattgcag cttataatgg ttacaaataa agcaatagca tcccaaattt cccaaataaa
     1261 gcattttttt ccctgcattc taatttgggg tttgtccaaa ctcatcaagg tatcttaacc
     1321 aggccgggaa ttgactcaaa ggatgtcaat tatcctatcc aga
//
