LOCUS       sequence-315566-        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA plasmid for amber suppression of N-terminally labelled 
            CoV2_M.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4x7SKPylT_EF1_Ub-*CoV2_M
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             110..378
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             417..644
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 tcacgggccc ttttccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
       61 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat
      121 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
      181 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
      241 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
      301 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt
      361 tctgcgccgt tacagatcca agctgtgacc ggcgcctact ctagagctag cgaattatgc
      421 agatcttcgt gaagactctg actggtaaga ccatcaccct cgaggttgag cccagtgaca
      481 ccatcgagaa tgtcaaggca aagatccaag ataaggaagg catccctcct gaccagcaga
      541 ggctgatctt tgctggaaaa cagctggaag atgggcgcac cctgtctgac tacaacatcc
      601 agaaagagtc caccctgcac ctggtgctcc gcttaagagg gggttaagca gactctaatg
      661 gtacaatcac ggttgaacaa cttaagaagt tgctggaaca atggaacctc gtgatagggt
      721 ttctcttttt gacgtggata tgcctcctcc aattcgccta tgcaaagaga cacagatttc
      781 tctacattat atagctcata ttcctttggc ttctctggcc ggtgactctt gcatgcttcg
      841 ttctggctgc ggtttacaga attaattgga ttaccggcgg gatcgccata gcaatggctt
      901 gcctcgacgg attgatgtga ctgtcatact gtattgtcag cttcagactg tttgtccgca
      961 caaggtctat gtggactttc aatccataaa cgaacatatt gcttaacgga cccctccacg
     1021 gtacgatatt gacccgactc ctgctcgcaa gctagt
//
