LOCUS       sequence-315567-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA plasmid for amber suppression of N-terminally labelled 
            CoV2_9b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             68..336
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             375..602
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(958..978)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gtcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa
       61 gcttcgaggg gctcgcatct ttccttcacg cgcccgccgc cctacctgag gccgccatcc
      121 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc
      181 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag
      241 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt
      301 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctactct
      361 agagctagcg aattatgcag atcttcgtga agactctgac tggtaagacc atcaccctcg
      421 aggttgagcc cagtgacacc atcgagaatg tcaaggcaaa gatccaagat aaggaaggca
      481 tccctcctga ccagcagagg ctgatctttg ctggaaaaca gctggaagat gggcgcaccc
      541 tgtctgacta caacatccag aaagagtcca ccctgcacct ggtgctccgc ttaagagggg
      601 gttaggatcc taaaatctct gagatgcacc ctgccttgcg attggtagac ccccaaatac
      661 agctcgccgt cacgagaatg gagaacgctg taggacgcga tcagaataac gtcggaccta
      721 aagtttatcc gattattttg agacttggca gtccgctctc ccttaacatg gctcgcaaaa
      781 ccctgaactc cctcgaagac aaagcatttc aacttacgcc catagctgtt cagatgacaa
      841 agttggcaac cacagaagag ctccccgacg aatttgtagt cgtgacggtt aaatgaccgg
      901 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
      961 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg cgttaactaa
     1021 acttgtttat tgcagcttaa tgaaag
//
