LOCUS       sequence-315568-        1234 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA plasmid for amber suppression of N-terminally labelled 
            CoV2_ORF6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6
FEATURES             Location/Qualifiers
     source          1..1234
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             108..376
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             415..642
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 cccggccctt ttccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
       61 ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct
      121 ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc
      181 tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc
      241 tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc
      301 tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc
      361 tgcgccgtta cagatccaag ctgtgaccgg cgcctactct agagctagcg aattatgcag
      421 atcttcgtga agactctgac tggtaagacc atcaccctcg aggttgagcc cagtgacacc
      481 atcgagaatg tcaaggcaaa gatccaagat aaggaaggca tccctcctga ccagcagagg
      541 ctgatctttg ctggaaaaca gctggaagat gggcgcaccc tgtctgacta caacatccag
      601 aaagagtcca ccctgcacct ggtgctccgc ttaagagggg gttagttcca cttggtggat
      661 ttccaagtca caatcgctga gatcctgctt attattatgc gaaccttcca aagtaagtat
      721 ctggaatttg gattatatta tcaacctgat aataaaaaat tttgtctaaa agtctgactg
      781 agaacaagta ctctcagctt gatgaagagc aacctatgga aatagactga ccggcgacaa
      841 tcaacctctg gattacaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
      901 tttaccctat gtgaaacgct gctttaatgc ctttgtatca tgcgttacta aacttggtta
      961 ttgcagctta aaatggttac aaataagcaa tagcatccca atttcccaat aaagcatttt
     1021 ttcctgcatc caagtggggt tggccaactc tcaaggatct tatcagtcgg gaatgaccca
     1081 aggatgcaat tatccttaaa aaaccttctg gggtcccttt gggggaaaaa aaacccttgt
     1141 ttaaatttaa aaaagggtcc caaaggggtt ttatatctct tttcttggga accattgtgg
     1201 ggttcccgcc ccacgaaaaa aacctaaaaa aaag
//
