LOCUS       sequence-31557-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Pk-SOD1
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     264..283
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     367..386
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        367..385
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    386..410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             425..447
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             466..483
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             496..516
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg
       61 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt tagtaggttg
      121 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
      181 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
      241 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
      301 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg
      361 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa
      421 taattttgtt taactttaag aaggagatat accatgggca gcagccatca tcatcatcat
      481 cacagcagcg gcagagaaaa cttgtatttc cagggcatgg caattattct acccaagttg
      541 aagtatgctt tgaacgccct gtctcctcat ataagtgaag agactctaaa cttccattac
      601 aataagcacc atgcggggta tgtgaacaag ttgaatggtc taataaagga tacgcccttt
      661 gccacgaaat cgttggtgg
//
