LOCUS       sequence-315570-        1159 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA plasmid for amber suppression of N-terminally labelled 
            CoV2_ORF7b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b
FEATURES             Location/Qualifiers
     source          1..1159
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             109..377
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             416..643
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(837..857)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    899..1020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(936..955)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     990..1009
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ccccggccct tttccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
       61 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
      121 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
      181 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
      241 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
      301 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
      361 ctgcgccgtt acagatccaa gctgtgaccg gcgcctactc tagagctagc gaattatgca
      421 gatcttcgtg aagactctga ctggtaagac catcaccctc gaggttgagc ccagtgacac
      481 catcgagaat gtcaaggcaa agatccaaga taaggaaggc atccctcctg accagcagag
      541 gctgatcttt gctggaaaac agctggaaga tgggcgcacc ctgtctgact acaacatcca
      601 gaaagagtcc accctgcacc tggtgctccg cttaagaggg ggttagatag aactgtcact
      661 gatcgacttt tatttgtgtt ttctcgcctt tttgttgttt ctcgttctca ttatgctcat
      721 catattttgg ttcagtctgg agttgcaaga ccacaatgaa acgtgtcatg cctgaccggc
      781 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
      841 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc gttaactaaa
      901 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
      961 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atggatctta
     1021 tcatgtctgg aattgactca atgatgcaat tagtccatcc aaaactcatc tgggctccct
     1081 tccgggggac agaaatcccg gtttaaattt aaaagcaggg ttcccaaatg gggtttttaa
     1141 tcccttgtct gggaaccag
//
