LOCUS       sequence-315571-        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA plasmid for amber suppression of N-terminally labelled 
            CoV2_ORF10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             109..377
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             416..643
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(822..842)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    884..1005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(921..940)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tcccggccct tttccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
       61 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
      121 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
      181 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
      241 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
      301 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
      361 ctgcgccgtt acagatccaa gctgtgaccg gcgcctactc tagagctagc gaattatgca
      421 gatcttcgtg aagactctga ctggtaagac catcaccctc gaggttgagc ccagtgacac
      481 catcgagaat gtcaaggcaa agatccaaga taaggaaggc atccctcctg accagcagag
      541 gctgatcttt gctggaaaac agctggaaga tgggcgcacc ctgtctgact acaacatcca
      601 gaaagagtcc accctgcacc tggtgctccg cttaagaggg ggttaggggt acataaatgt
      661 ttttgcattc cccttcacga tctatagttt gttgctgtgt cggatgaact ccagaaatta
      721 cattgctcaa gttgatgtgg tcaattttaa tctcacttga ccggcgacaa tcaacctctg
      781 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
      841 tgtggatacg ctgctttaat gcctttgtat catgcgttaa ctaaacttgt ttattgcagc
      901 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttgtc
      961 actgcattct agttgtgggt tgtccaaact catcaatgta tcttatcatg tctggaatga
     1021 ctcaatgatg tcagtattct atcagaagct catctggtct ccttccgggg gaaaagaatc
     1081 cctgtttaaa attttaacac caggt
//
