LOCUS       sequence-315572-        1421 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA plasmid for amber suppression of N-terminally labelled 
            CoV2_ORF9c/14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14
FEATURES             Location/Qualifiers
     source          1..1421
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             515..783
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             822..1049
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(1333..1353)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ccaaaataag tcgggggtgt tctggcgata cgggaagaca gatggtataa atagaaaaat
       61 aaaaaaaggt ttccccccgg gggaaaaaac acccccccca cccggggtgt tttttttttt
      121 ttgtgggcgg gcgcccaata atagaaattt ttttttgagt tttttttggt tcgggaattt
      181 aaattattac ccccccaatt cttttccaaa ataaatggaa aaggggtttt tcggttttgc
      241 cttcccaacc ctgaaggggg ggcttaaaat ccgaaaattg ttttaaaagt tttggtcttc
      301 cgggccgtca ggggcaaggc cccttccccc cagtcccgga agtgggggag ggtggcaatg
      361 aacggtgcct aggaagtggc cggggtaact ggaaagtgat gtcggtactg ctccgctttt
      421 tcccgagggt ggggagaacc gtatttagtg cagtagtcgc cgtgaacgtt cttttcgcaa
      481 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc
      541 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
      601 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
      661 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
      721 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag
      781 atccaagctg tgaccggcgc ctactctaga gctagcgaat tatgcagatc ttcgtgaaga
      841 ctctgactgg taagaccatc accctcgagg ttgagcccag tgacaccatc gagaatgtca
      901 aggcaaagat ccaagataag gaaggcatcc ctcctgacca gcagaggctg atctttgctg
      961 gaaaacagct ggaagatggg cgcaccctgt ctgactacaa catccagaaa gagtccaccc
     1021 tgcacctggt gctccgctta agagggggtt agcttcaatc ctgctacaac ttcctcaagg
     1081 aacaacactg tcaaaaggca tctacccaaa aaggggctga ggccgcagtt aaacctctgt
     1141 tggtaccaca tcacgtcgta gctacggtac aagaaattca gctccaagca gcagttggcg
     1201 aactgctcct gttggaatgg ctcgctatgg ctgtcatgct ccttcttctc tgttgctgct
     1261 tgacagattg accggcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     1321 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     1381 tcatgcgtta actaaacttg ttattgcagc taaataattt a
//
