LOCUS       sequence-315573-        1246 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA plasmid for amber suppression of N-terminally labelled 
            CoV2_ORF3b_alt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt
FEATURES             Location/Qualifiers
     source          1..1246
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        175..384
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             397..665
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             704..931
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(1167..1187)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 attttaaaaa tctttttccc ccccccaatt ttttttccca aaaataaaat tggaaaaagg
       61 gttgttttcc ggggtgtttg ccctttccca aatcccttca gaaggggggg gcctgttaaa
      121 tttggcaaaa ctaggcttta aaaaggatct gcgatcgttc cggtgccctt cagtggcaga
      181 ggccccttcg cccccattcc ccgagaagtg gggggagggg tcggcaattg aacgggtgcc
      241 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
      301 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
      361 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
      421 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
      481 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
      541 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
      601 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
      661 agatccaagc tgtgaccggc gcctactcta gagctagcga attatgcaga tcttcgtgaa
      721 gactctgact ggtaagacca tcaccctcga ggttgagccc agtgacacca tcgagaatgt
      781 caaggcaaag atccaagata aggaaggcat ccctcctgac cagcagaggc tgatctttgc
      841 tggaaaacag ctggaagatg ggcgcaccct gtctgactac aacatccaga aagagtccac
      901 cctgcacctg gtgctccgct taagaggggg ttaggcatac tgctggagat gcacaagctg
      961 ttgttttagt gagcgattcc agaatcacaa cccacaaaaa gaaatggcga cctcaacctt
     1021 gcagggatgc tcattgtgct tgcaactcgc ggtcgttgta tgcaatagcc tgcttacccc
     1081 tttcgctcga tgctgctggc cgtgaccggc gacaatcaac ctctggatta caaaatttgt
     1141 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     1201 ttaatgcctt tgtatcatgc gttaactaaa cattgtttat tgcagc
//
