LOCUS       sequence-315574-        1173 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  STELLA plasmid for amber suppression of N-terminally labelled 
            CoV2_ORF3b(CoV1h).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ
  TITLE     Universal Single-Residue Terminal Labels for Fluorescent Live Cell 
            Imaging of Microproteins.
  JOURNAL   J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 
            10.1021/jacs.0c09574. Epub 2020 Nov 11.
  PUBMED    33175524
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.0c09574"; journalName: "J Am Chem Soc"; date: 
            "2020-11-25- 25"; volume: "142"; issue: "47"; pages: "20080-20087"
COMMENT     Sequence Label: pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)
FEATURES             Location/Qualifiers
     source          1..1173
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             117..385
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             424..651
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             715..741
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(806..826)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    868..989
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(905..924)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     959..978
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aaattatgaa ccggcccttt tccgagggtg ggggagaacc gtatataagt gcagtagtcg
       61 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg
      121 ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga
      181 gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa
      241 gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac
      301 tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt
      361 tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctactcta gagctagcga
      421 attatgcaga tcttcgtgaa gactctgact ggtaagacca tcaccctcga ggttgagccc
      481 agtgacacca tcgagaatgt caaggcaaag atccaagata aggaaggcat ccctcctgac
      541 cagcagaggc tgatctttgc tggaaaacag ctggaagatg ggcgcaccct gtctgactac
      601 aacatccaga aagagtccac cctgcacctg gtgctccgct taagaggggg ttagccaact
      661 attttctttg ctggcatact aattgttacg actattgtat accttacaat agtgtaccca
      721 tacgatgttc cagattacgc ttaaccggcg acaatcaacc tctggattac aaaatttgtg
      781 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
      841 taatgccttt gtatcatgcg ttaactaaac ttgtttattg cagcttataa tggttacaaa
      901 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
      961 ggtttgtcca aactcatcaa tgtatcttat catgtctgga attgactcaa atgatgtcaa
     1021 ttagtctatc agaagctcat ctggtctccc ttccggggga caagaaatcc ctgtttaata
     1081 tttaaacagc agggttccca aactgggttc ttaattccct tggcctgggc aaccaggttg
     1141 cagggtttcc ggccccaagg aacaaatccc taa
//
