LOCUS       sequence-315576-        6305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0149:Dendra
FEATURES             Location/Qualifiers
     source          1..6305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2249..2272
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2529..3329
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3454..4042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3943..3962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgagcgctga ggcccactgt cccggcccgg ccccacaagg
      121 gccggaccgg gacaccggcg tcccaaccgg ccccataccg ggcagttcca agtgctaccg
      181 cgatcccgtc ggcgcacccg gcgggcgcgt gcccttccgt cgcgtacatc tgtcgggcgg
      241 aaggcatctc gacctctacg acacgtcggg gccctacacc gaaaccgaca cccccgcccg
      301 tctggccacg ggtctgccgt cgcgtcccgg tgtggtgacc gaccgtggca cccagttgca
      361 gcgcgcccgt gagggcgtcg tcaccagcga gatggcgttc gtcgccgccc gggaaagtct
      421 gcgccccaag gtggtccgcg acgcagtcgc cgcgggccac gccgtgatcc cggcgaatca
      481 ccgtcacccc gagagcgaac cgatgatcat cggcagggcg tttcgcgtca aggtcaacgc
      541 cggtatcggc tcgccgccgg acgcggtgat cggtgacatg gtgcgggcga tccggtgggg
      601 cgccgacatc gtgacggacc tgtccggcag cgactgcgac cgcatggtgc gcaacatgcc
      661 cgtgccggtg ggtacggtgc cgctgtacca ggcactcggc cgggtccacg gcgacatcgc
      721 cgcgttgacc tggaacacgt tctgcgacac cgtgatcgaa caggccgaga gcggagtcga
      781 ctgcatgacg gtgcatgccg gcctgcgccg cgaacatctg tcgctcgccg cgggccggat
      841 caccggcatc gtcagccgcg gcggggccgt catcgccgac tggtgcctgc tgcacaacgc
      901 ggaatccttt ctgtacgccc atttcgacca gctgtgcgag accctggcgc ggtacgacgt
      961 cacgctggcg ctggccgcgg gcctacagcc gggctctgtg gccgacgcca acgacgccgc
     1021 acagatcgcc gaactgcgca cgctgggtca gctggccagg gtcgcgcacc ggcacggcgt
     1081 gcaggtggtg gtcgaggggc cgggccgcgt gccgatgcac aagatcgccg agagcgtgcg
     1141 cattcatcag cagttgtgcc gcaatgcccc gtcgtacacc atcggcccac tcaccaccga
     1201 cgtcgcaccc gagcacgacc acgccaccgc ggttatcggc gccgccgtcg cggcccaggc
     1261 cggcacatcc atgctgtgct gcctgccccg gccagcccgc ctgcgcgaat cggtcatcgc
     1321 tcacaagatc gcggcgcatt cggcagatct ggccaagggg caccctcagg cccacgagca
     1381 cgatcacgcg atctcccggg cacgctgcga gacgcactgc cgcgaccggc tcgcgctctc
     1441 ccccgatccc gacgccgcgt tcaccggcca cgatcactcg aaggggagct acctgccgtt
     1501 tcctaagctt ggtgcggccg cgggcaacac gcctggcatc aatctgatca aggaggacat
     1561 gcgtgtcaag gtgcatatgg agggcaacgt gaacggtcac gccttcgtga tcgaggggga
     1621 gggtaagggt aagccttacg aagggaccca gaccgccaac ctcaccgtca aggagggcgc
     1681 ccctctccca ttctcgtacg atatcctcac taccgcggtg cattacggga accgcgtgtt
     1741 caccaagtac cccgaggata tccctgacta cttcaagcag agcttcccgg agggatactc
     1801 ctgggagcgg acgatgacct tcgaagacaa gggtatctgt acgatccgca gcgacatctc
     1861 gctggaaggg gactgtttct tccagaacgt ccgtttcaag gggaccaact ttccgccgaa
     1921 cggcccggtc atgcagaaga agaccctcaa gtgggagccg agcacggaga agctgcacgt
     1981 tcgggacggc ctcctggtcg gcaacatcaa catggcgctg ctgctggaag gcggcggcca
     2041 ctacctatgc gacttcaaga ccacgtacaa agcgaagaag gtcgtccagc tgcccgacgc
     2101 gcacttcgtg gaccaccgca tcgaaatcct ggggaacgat tcggactaca acaaggtcaa
     2161 gttgtacgag cacgctgtgg cacgctatag cccgctcccg tcgcaggtct ggttcgaagg
     2221 cggcggcggg gagaacctgt atttccagga ctacaaggac gacgacgaca agtgatttaa
     2281 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgttaacta gcgtacgatc
     2341 gaattcctgc agctcacggt aactgatgcc gtatttgcag taccagcgta cggcccacag
     2401 aatgatgtca cgctgaaaat gccggccttt gaatgggttc atgtgcagct ccatcagcaa
     2461 aaggggatga taagtttatc accaccgact atttgcaaca gtgccgttga tcgtgctatg
     2521 atcgactgat gtcatcagcg gtggagtgca atgtcgtgca atacgaatgg cgaaaagccg
     2581 agctcatcgg tcagcttctc aaccttgggg ttacccccgg cggtgtgctg ctggtccaca
     2641 gctccttccg tagcgtccgg cccctcgaag atgggccact tggactgatc gaggccctgc
     2701 gtgctacgct gggtccggga gggacgctcg tcatgccctc gtggtcaggt ctggacgacg
     2761 agccgttcga tcctgccacg tcgcccgtta caccggacct tggagttgtc tctgacacat
     2821 tctggcgcct gccaaatgta aagcgcagcg cccatccatt tgcctttgcg gcagcggggc
     2881 cacaggcaga gcagatcatc tctgatccat tgcccctgcc accttactcg cctgcaagcc
     2941 cggtcgcccg tgtccatgaa ctcgatgggc aggtacttct cctcggcgtg ggacacgatg
     3001 ccaacacgac gctgcatctt gccgagttga tggcaaaggt tccctatggg gtgccgagac
     3061 actgcaccat tcttcaggat ggcaagttgg tacgcgtcga ttatctcgag aatgaccact
     3121 gctgtgagcg ctttgccttg gcggacaggt ggctcaagga gaagagcctt cagaaggaag
     3181 gtccagtcgg tcatgccttt gctcggttga tccgctcccg cgacattgtg gcgacagccc
     3241 tgggtcaact gggccgagat ccgttgatct tcctgcatcc gccagaggcg ggatgcgaag
     3301 aatgcgatgc cgctcgccag tcgattggct gagctcatga gcggagaacg agatgacgtt
     3361 ggaggggcaa ggtcgcgctg attgctgggg caacacgggg gatccactag ttccatgagc
     3421 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3481 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3541 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3601 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3661 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3721 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3781 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3841 tgagctatga gaaagcgcca cgcttcccga ggggagaaag gcggacaggt atccggtaag
     3901 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3961 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4021 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4081 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4141 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc
     4201 gtgcggccgc ggtacccggg gatcctctag agtcgaccac caagggcacc atctctgctt
     4261 gggccacccc gttggccgca gccagctcgc tgagagccgt gaacgacagg gcgaacgcca
     4321 gcccgccgac ggcgagggtt ccgaccgctg caactcccgg tgcaaccttg tcccggtcta
     4381 ttctcttcac tgcaccagct ccaatctggt gtgaatgccc ctcgtctgtt cgcgcaggcg
     4441 gggggctcta ttcgtttgtc agcatcgaaa gtagccagat cagggatgcg ttgcaaccgc
     4501 gtatgcccag gtcagaagag tcgcacaaga gttgcagacc cctggaaaga aaaatggcca
     4561 gagggcgaaa acaccctctg accagcggag cgggcgacgg gaatcgaacc cgcgtagcta
     4621 gtttggaaga atgggtgtct gccgaccaca tatgggccgg tcaagatagg tttttacccc
     4681 ctctcggctg catcctctaa gtggaaagaa attgcaggtc gtagaagcgc gttgaagcct
     4741 gagagttgca caggagttgc aacccggtag ccttgttcac gacgagagga gacctagttg
     4801 gcacgtcgcg gatggggatc gctgaagact cagcgcagcg ggaggatcca agcctcatac
     4861 gtcaacccgc aggacggtgt gaggtactac gcgctgcaga cctacgacaa caagatggac
     4921 gccgaagcct ggctcgcggg cgagaagcgg ctcatcgaga tggagacctg gacccctcca
     4981 caggaccggg cgaagaaggc agccgccagc gccatcacgc tggaggagta cacccggaag
     5041 tggctcgtgg agcgcgacct cgcagacggc accagggatc tgtacagcgg gcacgcggag
     5101 cgccgcatct acccggtgct aggtgaagtg gcggtcacag agatgacgcc agctctggtg
     5161 cgtgcgtggt gggccgggat gggtaggaag cacccgactg cccgccggca tgcctacaac
     5221 gtcctccggg cggtgatgaa cacagcggtc gaggacaagc tgatcgcaga gaacccgtgc
     5281 cggatcgagc agaaggcagc cgatgagcgc gacgtagagg cgctgacgcc tgaggagctg
     5341 gacatcgtcg ccgctgagat cttcgagcac taccggatcg cggcatacat cctggcgtgg
     5401 acgagcctcc ggttcggaga gctgatcgag cttcgccgca aggacatcgt ggacgacggc
     5461 atgacgatga agctccgggt gcgccgtggc gcttcccgcg tggggaacaa gatcgtcgtt
     5521 ggcaacgcca agaccgtccg gtcgaagcgt cctgtgacgg ttccgcctca cgtcgcggag
     5581 atgatccgag cgcacatgaa ggaccgtacg aagatgaaca agggccccga ggcattcctg
     5641 gtgaccacga cgcagggcaa ccggctgtcg aagtccgcgt tcaccaagtc gctgaagcgt
     5701 ggctacgcca agatcggtcg gccggaactc cgcatccacg acctccgcgc tgtcggcgct
     5761 acgttcgccg ctcaggcagg tgcgacgacc aaggagctga tggcccgtct cggtcacacg
     5821 actcctagga tggcgatgaa gtaccagatg gcgtctgagg cccgcgacga ggctatcgct
     5881 gaggcgatgt ccaagctggc caagacctcc tgaaacgcaa aaagcccccc tcccaaggac
     5941 actgagtcct aaagaggggg gtttcttgtc agtacgcgaa gaaccacgcc tggccgcgag
     6001 cgccagcacc gccgctctgt gcggagacct gggcaccagc cccgccgccg ccaggagcat
     6061 tgccgttccc gccagaaatc tagacggtga ccacaacgcg cccgctttga tcggggacgt
     6121 cgaaatattg gatcgtcggc accgtcacgg ccgtgggagg cggcacgatc cgcgacgtga
     6181 tgatcggccg catccccacg gtgctgcgca gtgagctcta cgccatcccg gcgttgatct
     6241 gtgcgttcgc acgcacaggc ccggtgtgag aagggtctct gacgagcggg agaactccct
     6301 atcag
//
